Rhizobium etli CFN42 contains at least three plasmids of the repABC family: a structural and evolutionary analysis

Cevallos, Miguel A.; Porta, Helena; Izquierdo, Javier A.; Tun-Garrido, Cristina; Garcı́a-de-los-Santos, Alejandro; Dávila, Guillermo; Brom, Susana

doi:10.1016/s0147-619x(02)00119-1

Cited by 40 publications

(22 citation statements)

References 42 publications

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“…The best characterized repABC cassettes are found on the symbiosis plasmids p42d of Rhizobium etli and pSymA from Sinorhizobium meliloti, on two different Ti plasmids from A. tumefaciens and on plasmid pTAV1 from Paracoccus versutus 12,[18][19][20][21][22][23][24] . All of these model organisms, except for P. versutus, are members of the order Rhizobiales.…”

Section: Distribution Of Repabc-type Systemsmentioning

confidence: 99%

The ABCs of plasmid replication and segregation

2012

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To ensure faithful transmission of low-copy plasmids to daughter cells, these plasmids must replicate once per cell cycle and distribute the replicated DNA to the nascent daughter cells. RepABC family plasmids are found exclusively in alphaproteobacteria and carry a combined replication and partitioning locus, the repABC cassette, which is also found on secondary chromosomes in this group. RepC and a replication origin are essential for plasmid replication, and RepA, RepB and the partitioning sites distribute the replicons to predivisional cells. Here, we review our current understanding of the transcriptional and post-transcriptional regulation of the Rep proteins and of their functions in plasmid replication and partitioning.

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Section: Distribution Of Repabc-type Systemsmentioning

confidence: 99%

The ABCs of plasmid replication and segregation

2012

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“…strains (Li and Farrand, 2000) and the second chromosome in the animal pathogen Brucella (Paulsen et al, 2002). The repABC genes also appear to be widely distributed on accessory plasmids in several rhizobial species (Cevallos et al, 2002;Stiens et al, 2006;Watson and Heys, 2006). Amino-acid sequence analysis revealed that two ORFs, BBta_p0014 and BBta_p0015, located downstream of the repABC replication module, were 42% and 35% identical to ParA-like ATPase and ParBlike partition protein from S. meliloti plasmid pMBA19a (accession number AY914874) and the Rhodobacter sphaeroides plasmid pRSPA04 (accession number NC_009432), respectively.…”

Section: The Repabc Replicon Of Plasmid Pbtai1mentioning

confidence: 99%

Insights learned from pBTAi1, a 229-kb accessory plasmid from Bradyrhizobium sp. strain BTAi1 and prevalence of accessory plasmids in other Bradyrhizobium sp. strains

et al. 2008

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In silico, physiological and in planta analyses were used to characterize pBTAi1, a 229-kb accessory plasmid from Bradyrhizobium sp. strain BTAi1, and assess its potential ecological function under free-living and symbiotic growth conditions. Sequence analysis revealed the presence of an uptake hydrogenase system, a repABC family plasmid replication module and open reading frames encoding type IV secretion system, TraI and TraR autoinducer proteins and several copper resistance-related proteins. Bradyrhizobium sp. BTAi1 was capable of growing in 200 mg l À1 CuCl 2 . In contrast, the closely related, plasmid-free Bradyrhizobium sp. strain ORS278 could not grow at copper concentrations exceeding 100 mg l À1 . The plasmid-localized hydrogenase genes were phylogenetically distinct from those typically found in other rhizobial species, and were most related to hup genes from Thiobacillus denitrificans. The induction of the plasmid-borne hydrogenase genes during symbiosis was significantly lower than the two chromosomal-borne hydrogenase clusters. CHEF-pulsed-field gel electrophoresis was used for a comprehensive analysis of the diversity, abundance and genetic composition of accessory plasmids in other Bradyrhizobium strains. Plasmids were detected in 11 of 46 (23.9%) geographically diverse Bradyrhizobium japonicum and Bradyrhizobium elkanii strains, isolated from the United States, China and Thailand. Plasmid size was heterogeneous, ranging from 75 to 330 kb, with only two strains (DASA01244 and DASA01265) harboring plasmids with identical (240 kb) size. None of the plasmids harbored nodulation or hydrogenase genes. Taken together, our results indicate that while plasmids having ecologically significant functions may be detected in Bradyrhizobium sp. strains, they lack genes necessary for symbioses with legumes.

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“…Firstly, the maintenance of multiple plasmids in the cell indicates the presence of several incompatibility groups within the repABC plasmid family [19,30,48]. Secondly, phylogenetic analyses of rep regions showed that repABC plasmids coexisting in the same strain most probably arose through separate events of lateral gene transfer, further forcing the appearance of different new incompatibility groups and allowing co-residence of plasmids equipped with the same type of replication/ partition machinery in one bacterial cell [30,48,49]. In regards to the evolution of the repABC operon itself, it was hypothesized that it presumably originated through integration of the ctRNA-repC cassette between the repAB genes and the par-site located immediately downstream of the repB, separating the repAB genes from the parS.…”

Section: Rhizobial Plasmid Maintenance Systemsmentioning

confidence: 99%

Rhizobial plasmids — replication, structure and biological role

Mazur

Koper

2012

Open Life Sciences

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AbstractSoil bacteria, collectively named rhizobia, can establish mutualistic relationships with legume plants. Rhizobia often have multipartite genome architecture with a chromosome and several extrachromosomal replicons making these bacteria a perfect candidate for plasmid biology studies. Rhizobial plasmids are maintained in the cells using a tightly controlled and uniquely organized replication system. Completion of several rhizobial genome-sequencing projects has changed the view that their genomes are simply composed of the chromosome and cryptic plasmids. The genetic content of plasmids and the presence of some important (or even essential) genes contribute to the capability of environmental adaptation and competitiveness with other bacteria. On the other hand, their mosaic structure results in the plasticity of the genome and demonstrates a complex evolutionary history of plasmids. In this review, a genomic perspective was employed for discussion of several aspects regarding rhizobial plasmids comprising structure, replication, genetic content, and biological role. A special emphasis was placed on current post-genomic knowledge concerning plasmids, which has enriched the view of the entire bacterial genome organization by the discovery of plasmids with a potential chromosome-like role.

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Rhizobium etli CFN42 contains at least three plasmids of the repABC family: a structural and evolutionary analysis

Cited by 40 publications

References 42 publications

The ABCs of plasmid replication and segregation

The ABCs of plasmid replication and segregation

Insights learned from pBTAi1, a 229-kb accessory plasmid from Bradyrhizobium sp. strain BTAi1 and prevalence of accessory plasmids in other Bradyrhizobium sp. strains

Rhizobial plasmids — replication, structure and biological role

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