Rhizoma Paridis total saponins alleviate H2O2‑induced oxidative stress injury by upregulating the Nrf2 pathway

Abstract

Rhizoma Paridis total saponins (RPTS) is an active substance isolated from the traditional Chinese medicine Rhizoma Paridis, which possesses multiple biological activities. The aim of the present study was to explore the roles and mechanisms of RPTS in oxidative stress injury of ARPE-19 human retinal pigment epithelial cells. Cell viability, reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP) and apoptosis were determined by Cell Counting kit-8 assay and flow cytometry, respectively. Enzyme-linked immunosorbent assay was performed to detect the expression of oxidative stress markers. Western blotting and reverse transcription-quantitative polymerase chain reaction were used to determine the expression levels of related genes and proteins. The results revealed that RPTS enhanced cell viability and reduced H2O2-induced oxidative stress of ARPE-19 human retinal pigment epithelial cells. RPTS increased the MMP of ARPE-19 cells compared with in H2O2-treated ARPE-19 cells. In addition, RPTS suppressed ROS production and apoptosis of H2O2-treated ARPE-19 cells. Additionally, RPTS modulated the expression levels of apoptosis-associated proteins and the nuclear factor 2-related factor 2 (Nrf2) pathway. In conclusion, RPTS alleviated H2O2-induced oxidative stress injury by upregulating the Nrf2 pathway. The potential effects of RPTS on protection against H2O2-induced apoptosis of ARPE-19 cells suggested that RPTS may be a potential therapeutic target for preventing age-related macular degeneration.

Modified rougan decoction alleviates lipopolysaccharide-enrofloxacin-induced hepatotoxicity via activating the Nrf2/ARE pathway in chicken

“…In our study, the results showed that Nrf2 was blocked into the nucleus in the LPS-ENR group ( Figure 4 ), while the transfer of Nrf2, the mRNA expression level of Nrf2, and the protein expression level of Nrf2 were improved by MRGD and MRGDE ( Figure 3 A and B). Once in the nucleus, Nrf2 bonded to the ARE, thereby activating the mRNA expressions of several downstream genes such as HO-1 and NQO1 in the nucleus ( Zhang et al, 2019 ; Zhao et al, 2020 ). A report showed that trehalose adjusts the REDOX in cells via activating the Nrf2 pathway and increasing the mRNA expression levels of genes GPX1, CAT, SOD1, NQO1, and HO-1 ( Xie et al, 2017 ).…”

Punicalagin Protects Human Retinal Pigment Epithelium Cells from Ultraviolet Radiation-Induced Oxidative Damage by Activating Nrf2/HO-1 Signaling Pathway and Reducing Apoptosis

“…In addition, our evidence fully introduces PUN among antioxidant natural substances able to protect RPE from oxidative stress. In this regard, there is a wide research line that describes compounds of natural origin capable of protecting RPE, via Nrf2 activation, against various oxidative stimuli (mainly H 2 O 2 ) [ 29 , 30 , 31 ]. However, the fact that, in our experimental paradigm, PUN protects epithelium against UV radiation (the main natural exogenous stressor for RPE) makes it particularly noteworthy, compared to other compounds.…”

Tribulus terrestris Ameliorates Oxidative Stress-Induced ARPE-19 Cell Injury through the PI3K/Akt-Nrf2 Signaling Pathway

“…Numerous reports have proved that Nrf2 is an emerging regulator of cellular resistance to oxidative stress. Some natural products (such as apigenin, quercetin, gypenosides, genipin, rhizoma paridis, escin, and astaxanthin) have also been shown to have protective effects against the oxidative stressed ARPE-19 cells associated with Nrf2 signaling [28,[57][58][59][60][61][62][63]. In our study, we found that treatment with 1 mM H 2 O 2 for 24 h significantly induced a serious oxidative cell damage and apoptosis in ARPE-19 cells (Figures 1 and 2).…”