Rhizospheric bacterial isolates of grass pea (<i>Lathyrus sativus</i>L.) endowed with multiple plant growth promoting traits

Mussa, A. E. A.; Million, T.; Assefa, Fassil

doi:10.1111/jam.13942

Cited by 21 publications

(14 citation statements)

References 49 publications

(77 reference statements)

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“…In this study, the production of plant growth-promoting trait by Enterococcus spp. agreed with the study of (Anzuay et al 2017;Mussa et al 2018) that reported the isolation of Enterococcus spp growth-promoting potential and antifungal activity against pathogenic fungi from the rhizosphere of grass pea. Massilia spp.…”

Section: Discussionsupporting

confidence: 91%

Characterization of plant growth-promoting rhizobacterial isolates associated with food plants in South Africa

Fasusi

Amoo

Babalola

2021

Antonie van Leeuwenhoek

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The region around the plant root referred to as the rhizosphere, is the zone where various microbial activity occurs. It performs crucial functions such as increasing the uptake of nutrients for plant development and preventing plant against plant pathogens. Keeping in mind the beneficial role performed by rhizospheric microorganisms, rhizobacterial species were isolated from the maize and soybean plant's rhizosphere. The isolated microorganisms were evaluated for their biochemical characteristics, plant growth-promoting potentials, tolerance to different environmental conditions, and their antifungal activity against Fusarium graminearum, a fungal pathogen that infects maize. The rhizobacterial isolates with multiple plant growth-promoting potentials were identified as Bacillus spp (80.77%), Rhodocyclaceae bacterium (3.85%), Enterococcus spp (3.85%). Massilia spp (3.85%. and Pseudomonas (7.69%) species based on their 16S rRNA molecular characterization. The bacterial isolates possessed antifungal activities against Fusarium graminearum, promote maize and soybeans seed under laboratory conditions, and exhibited different levels of tolerance to pH, temperature, salt, and heavy metal. Based on this, the whole genome sequencing of Bacillus sp. OA1, Pseudomonas rhizosphaerea OA2, and Pseudomonas sp. OA3 was performed using Miseq Illumina system to determine the functional genes and secondary metabolites responsible for their plant growth-promoting potential Thus, the result of this research revealed that the selected bacterial isolates possess plant growth-promoting potentials that can make them a potential candidate to be employed as microbial inoculants for protecting plants against phytopathogens, environmental stress and increasing plant growth and productivity.

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Section: Discussionsupporting

confidence: 91%

Characterization of plant growth-promoting rhizobacterial isolates associated with food plants in South Africa

Fasusi

Amoo

Babalola

2021

Antonie van Leeuwenhoek

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“…No isolate produced IAA in physiologically relevant concentrations, in agreement with previous studies which also included also LAB [ 69 , 70 ]. On the other hand, Mussa et al [ 71 ] reported that one strain of Enterococcus casseliflavus , isolated from grass pea rhizospheric soil, synthesized IAA at a concentration of ca. 56 µg mL −1 , corresponding to about one order of magnitude higher than the best IAA producer among the LAB subjected to this research.…”

Section: Discussionmentioning

confidence: 99%

“…Under our experimental conditions, all the 16 LAB strains solubilized high amount of phosphate, comparable to strains of Enterococcus sp. isolated from grass pea rhizospheric soil [ 71 ]. The release of organic acids from LAB and the consequent acidification of broth (4.5 < pH < 4.9) could explain the observed solubilization of P. Indeed, previous researches showed inverse correlation between phosphate solubilization and pH of culture broth, varying from weak–moderate [ 71 ] to strong [ 80 ].…”

Section: Discussionmentioning

confidence: 99%

“…isolated from grass pea rhizospheric soil [ 71 ]. The release of organic acids from LAB and the consequent acidification of broth (4.5 < pH < 4.9) could explain the observed solubilization of P. Indeed, previous researches showed inverse correlation between phosphate solubilization and pH of culture broth, varying from weak–moderate [ 71 ] to strong [ 80 ]. On the other hand, de Lacerda et al [ 81 ] reported no significant correlation between pH and calcium phosphate solubilization.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere

et al. 2020

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This study aimed to isolate lactic acid bacteria (LAB) from wheat rhizosphere, to characterize their in vitro plant growth promoting activities and to differentiate plant-associated LAB from those associated with foods or human disease through comparative genomic analysis. Lactococcus lactis subsp. lactis and Enterococcus faecium were isolated using de Man-Rogosa-Sharpe (MRS) and Glucose Yeast Peptone (GYP) as enrichment culture media. Comparative genomic analyses showed that plant-associated LAB strains were enriched in genes coding for bacteriocin production when compared to strains from other ecosystems. Isolates of L. lactis and E. faecium did not produce physiologically relevant concentrations of the phyto-hormone indolacetic acid. All isolates solubilized high amount of phosphate and 12 of 16 strains solubilized potassium. E. faecium LB5, L. lactis LB6, LB7, and LB9 inhibited the plant pathogenic Fusarium graminearum to the same extent as two strains of Bacillus sp. However, the antifungal activity of the abovementioned LAB strains depended on the medium of cultivation and a low pH while antifungal activity of Bacillus spp. was independent of the growth medium and likely relates to antifungal lipopeptides. This study showed the potential of rhizospheric LAB for future application as biofertilizers in agriculture.

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“…The different proportions of grasspea protein isolates and glycerol were used to combine Lepidium perfoliatum L. seed gum to form composite biopolymer films that are biodegradable in nature ( 91 ). An effective biocontrol strain of plant growth promoting rhizobacteria obtained from the rhizosphere of grasspea was used as an alternative to conventional fertilizer that could contribute to crop disease reduction and significantly increase crop growth and yield ( 92 ). The ability of this crop to withstand nutrient shortage and retain vast amounts of lead in the root tissues has made it a tough species.…”

Section: Abandoned Neglected and Orphan Legume With Multiple Usesmentioning

confidence: 99%

Rediscovering the Potential of Multifaceted Orphan Legume Grasspea- a Sustainable Resource With High Nutritional Values

et al. 2022

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The genus Lathyrus consists of more than 184 herbaceous annual and perennial species suitable for multifaceted sustainable food and feed production system in the arid and semi-arid regions of the world. The grasspea is a promising source of protein nutrition. However, its potential is not being utilized fully due to the presence of neurotoxin content (β-N-oxalyl-l-α, β diaminopropionic acid, β-ODAP), a causal agent of non-reversible lower limbs paralysis. The high protein contents in seeds and leaves with ~90% digestibility make it sustainable super food to beat protein malnutrition in future. Therefore, it is desired to breed new grasspea cultivars with low β-ODAP contents. Limited research has been carried out to date about this feature. A draft genome sequence of grasspea has been recently published that is expected to play a vital role in breeding and identifying the genes responsible for biosynthesis pathway of β-ODAP contents in grasspea. Efforts to increase awareness about the importance of genus Lathyrus and detoxify β-ODAP in grasspea are desired and are in progress. Presently, in South Asia, systematic and dedicated efforts to support the farmers in the grasspea growing regions by disseminating low β-ODAP varieties has resulted in a considerable improvement in reducing the incidence of neurolathyrism. It is expected that the situation will improve further by mainstreaming grasspea cultivation by implementing different approaches such as the development and use of low β-ODAP varieties, strengthening government policies and improved detox methods. The present review provides insight into the multifaceted characteristics of sustainable nutritious grasspea in the global and Indian perspective.

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Rhizospheric bacterial isolates of grass pea (Lathyrus sativusL.) endowed with multiple plant growth promoting traits

Abstract: This study illustrates an effective alternative to conventional fertilizers that may contribute to crop disease reduction. Our results provide a foundation for future research that will lead to identifying potentially useful biocontrol strains found in the rhizosphere of grass pea.

Cited by 21 publications

References 49 publications

Characterization of plant growth-promoting rhizobacterial isolates associated with food plants in South Africa

Characterization of plant growth-promoting rhizobacterial isolates associated with food plants in South Africa

Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere

Rediscovering the Potential of Multifaceted Orphan Legume Grasspea- a Sustainable Resource With High Nutritional Values

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