Rhizospheric soil fungal community patterns of Duchesnea indica in response to altitude gradient in Yunnan, southwest China

Abstract

The magnitude of the impact of altitude gradient on microbial community and diversity has been studied in recent decades. Whereas bacteria have been the focus of most studies, fungi have been given relatively less attention. As a vital part of the macro- and microscopic ecosystem, rhizosphere fungi play a key role in organic matter decomposition and relative abundance of plant species and have an impact on plant growth and development. Using Duchesnea indica as the host plant, we examined the rhizosphere soil fungal community patterns across the altitude gradient in 15 sites of Yunnan province by sequencing the fungal ITS2 region with the Illumina MiSeq platform. We determined the fungal community composition and structure. We found that, surprisingly, rhizosphere soil fungal diversity of D. indica increased with altitudinal gradient. There was a slight difference in diversity between samples from high- and medium-altitude sites, with medium-altitude sites having the greater diversity. Furthermore, the rhizosphere soil fungal community composition and structure kept changing along the altitudinal gradient. Taxonomic results showed that the extent of phylum diversity was greatest at high-altitude sites, with Ascomycota, Basidiomycota, Zygomycota, and Glomeromycota as the most dominant fungal phyla.

Altitudinal Distribution Patterns of Phyllosphere Microbial Communities and Their Contribution to Silage Fermentation of Kobresia pygmaea Along the Elevation Gradient on the Tibetan Plateau

“…For the fungi, Pyrenocchaetopsis are present in fresh K. pygmaea from elevations below 4,000 m a.s.l., and its RA decreased with the increasing elevation. Pyrenochaetopsis was one of the rhizospheric soil fungal genera in low-altitude sites rather than high- and medium-altitude sites (Jamil et al, 2020 ). Protomyces and Leucosporidium are present in fresh K. pygmaea from elevations below 4,000 m a.s.l.…”

Differences in Microbial Communities Stimulated by Malic Acid Have the Potential to Improve Nutrient Absorption and Fruit Quality of Grapes

“…The V3-V4 hypervariable region of the bacterial 16S rRNA gene ( Wan et al, 2018 ) was amplified with the primer pairs 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′). The fungal-specific primers ( Jamil et al, 2020 ) ITS3F (5′-GATGAAGAACGYAGYRAA-3′) and ITS4R (5′-TCCTCCG CYYATTGATATGC-3′) were employed to amplify the fungal ITS region. Polymerase chain reaction (PCR) amplification of the 16S rRNA gene was performed as follows: initial denaturation at 95°C for 3 min, followed by 27 cycles of denaturing at 95°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 45 s, a single extension at 72°C for 10 min and ending at 4°C.…”

Ecology and potential functions of plant-associated microbial communities in cold environments

“… 2019 ; Jamil et al . 2020 ), Arctic (Walker et al . 2011 ; Nissinen, Männistö and van Elsas 2012 ; Blaalid et al .…”

“… 2018 ; Praeg, Pauli and Illmer 2019 ; Jamil et al . 2020 ). The dominant fungal genera were Psathyrella , Armillaria , Sordariales , Helotiales and Cylindrocarpon in Stipa purpurea (Lu et al .…”

“…Mortierella , Gibberella , Cilliophora and Zopifiella ) were consistently found across three different altitude gradients (Jamil et al . 2020 ). Furthermore, ectomycorrhizal communities in alpine regions were dominated by Cenococcum , Thelephoraceae and Cortinarius genera (Ryberg, Larsson and Molau 2009 ).…”