Rho-dependent transcription termination: a revisionist view

Hao, Zhimin; Svetlov, Vladimir; Nudler, Evgeny

doi:10.1080/21541264.2021.1991773

Cited by 31 publications

(25 citation statements)

References 80 publications

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Section: Main Textmentioning

confidence: 99%

“…Recently, two research teams reported cryo-EM structures of Rho-TEC complexes assembled on synthetic nucleic-acid scaffolds ( 31-33 ). However, the reported structures differed from expectation based on genetic and biochemical experiments in crucial respects ( 4, 31-33 ). The structures did not show the expected, catalytically competent, closed-ring state of Rho, did not show the expected protein-RNA interactions with the Rho PBS and Rho SBS, did not show the expected threading of RNA through Rho, did not show the expected orientation of Rho relative to the TEC, and did not show the expected interaction between Rho and NusG ( 4, 31-33 ).…”

Section: Main Textmentioning

confidence: 99%

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Structural basis of Rho-dependent transcription termination

Molodtsov

Wang

Firlar

et al. 2022

Preprint

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Rho and NusG mediate factor-dependent transcription termination in Escherichia coli. Here, we report preparation of complexes functional in factor-dependent termination from RNA polymerase (RNAP), Rho, NusG, and synthetic nucleic-acid scaffolds, and we report cryo-EM structures of complexes. The structures show that functional factor-dependent pre-termination complexes contain a closed-ring Rho hexamer, have RNA threaded through the central channel of Rho, have 60 nt of RNA interacting sequence-specifically with the exterior of Rho and 6 nt of RNA interacting sequence-specifically with the central channel of Rho, have Rho oriented relative to RNAP such that ATP-hydrolysis-dependent translocation by Rho exerts mechanical force on RNAP, and have NusG bridging Rho and RNAP. The results explain five decades of research on Rho and provide a foundation for understanding Rho function.

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Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Structural basis of Rho-dependent transcription termination

Molodtsov

Wang

Firlar

et al. 2022

Preprint

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“…The bacterial motor protein Rho is widely used to control expression of metabolic or stress response genes (31). Rho-utilization (rut) sites are unstructured mRNA regions to which Rho can bind, composed of repeated C-rich patterns (32,33). Analyses of the nucleotide sequence of the locus revealed a so-called C .…”

Section: Figmentioning

confidence: 99%

Posttranscriptional Regulation by Copper with a New Upstream Open Reading Frame

Roy

Antoine

Schwartz

et al. 2022

mBio

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“…The bacterial motor protein Rho is widely used to control expression of metabolic or stress response genes (Turnbough, 2019). Rho-utilization (rut) sites are unstructured mRNA regions to which Rho can bind, composed of repeated, C-rich patterns (Hao et al, 2021, Nadiras et al, 2018. Analyses of the nucleotide sequence of the locus revealed a so-called C>G bubble, i.e., a region where the percentage of C is higher than that of G on the coding strand, starting in the unstructured region of bp2923 and ending in IGR (Fig.…”

Section: Rho-dependent Transcription Termination Of the Operonmentioning

confidence: 99%

Post-transcriptional regulation by copper with a new upstream Open Reading Frame

Roy

Antoine

Schwartz

et al. 2022

Preprint

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Copper is essential to most living beings but also toxic. Bacteria have thus developed homeostatic mechanisms to tightly control its intracellular concentration. The 3-gene operon bp2923-bfrG-bp2921 is down-regulated by copper and notably encodes a TonB-dependent transporter in Bordetella pertussis. We show that the protein encoded by bp2923, which is a member of the DUF2946 family, represents a new type of upstream Open Reading Frame (uORF) involved in post-transcriptional regulation of the downstream genes. In the absence of copper, the entire operon is transcribed and translated. Perception of copper by the nascent bp2923-coded protein via its conserved CXXC motif triggers Rho-dependent transcription termination between the first and second genes by relieving translation arrest on a conserved C-terminal RAPP motif. Homologues of bp2923 are widespread in bacterial genomes, where they head operons predicted to participate in copper homeostasis. This work has unveiled an original mode of genetic regulation by a transition metal and identified a regulatory function for a member of an uncharacterized family of bacterial proteins that we have named CruR, for copper-responsive upstream regulator.

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Rho-dependent transcription termination: a revisionist view

Cited by 31 publications

References 80 publications

Structural basis of Rho-dependent transcription termination

Structural basis of Rho-dependent transcription termination

Posttranscriptional Regulation by Copper with a New Upstream Open Reading Frame

Post-transcriptional regulation by copper with a new upstream Open Reading Frame

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