Rho signals to cell growth and apoptosis

Aznar, Salvador; Lacal, Juan Carlos

doi:10.1016/s0304-3835(01)00412-8

Cited by 286 publications

(209 citation statements)

References 74 publications

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“…Rac1 and Cdc42 can syner gize with Raf to activate ERK [25][26][27]. Furthermore, Rac1 cooperates with PI-3 kinase in controlling cell migration and polarity [28]. Interestingly, these small GTPases are found to mediate apoptosis in a wide-range of cell types.…”

Section: Introductionmentioning

confidence: 99%

Statins induce apoptosis in ovarian cancer cells through activation of JNK and enhancement of Bim expression

Liu

Liang

Kumar

et al. 2008

Cancer Chemother Pharmacol

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Purpose Ovarian cancer is the leading cause of death among all gynecological malignancies in Western coun tries. Although therapy for ovarian cancer has been greatly improved in the past 20 years, the overall survival for patients with advanced ovarian cancer has not changed sig niWcantly. The poor survival rates in patients with advanced ovarian cancer are due both to late diagnosis and to lack of eVective drugs for the majority of patients who have a relapse and develop resistance to current chemotherapy agents used for ovarian cancer. Thus, developing and dis covering eVective novel drugs with diVerent molecular structures from conventional chemotherapy agents have become an urgent clinical need.

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Section: Introductionmentioning

confidence: 99%

Statins induce apoptosis in ovarian cancer cells through activation of JNK and enhancement of Bim expression

Liu

Liang

Kumar

et al. 2008

Cancer Chemother Pharmacol

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“…Also, RhoA upregulates anti-apoptotic Bcl-2 expression in T cells (Gomez et al, 1997), vascular smooth muscle cells (Blanco-Colio et al, 2002) and osteosarcoma cells (Fromigue et al, 2006). On the contrary, it has been reported that activation of RhoA-ROCK pathway also induces apoptosis (Aznar and Lacal, 2001;Coleman et al, 2001). However, all these studies were restricted to in vitro culture system.…”

Section: Introductionmentioning

confidence: 99%

RhoA prevents apoptosis during zebrafish embryogenesis through activation of Mek/Erk pathway

et al. 2007

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RhoA small GTPase, as a key regulator for actin cytoskeletal rearrangement, plays pivotal roles during morphogenesis, cytokinesis, phagocytosis and cell migration, but little is known about its signaling mechanism that controls cell survival in vivo. Using zebrafish as a model, we show that non-overlapping antisense morpholinos that block either translation or splicing of rhoA lead to extensive apoptosis during embryogenesis, resulting in overall reduction of body size and body length. These defects are associated with reduced activation of growth-promoting Erk and decreased expression of anti-apoptotic bcl-2. Moreover, ectopic expression of rhoA, Mek or BCL-2 mRNA rescues such phenotypes. Consistently, combined suppression of RhoA and Mek/Erk or Bcl-2 pathways by sub-optimal dose of rhoA morpholino and pharmacological inhibitors for either Mek (U0126) or Bcl-2 (HA 14-1) can induce developmental abnormalities and enhanced apoptosis, similar to those caused by effective RhoA knockdown. Furthermore, U0126 abrogates the rescue by RhoA and MEK but not BCL-2. In contrast, HA 14-1 effectively abolishes all functional rescues by RhoA, MEK or BCL-2, supporting that RhoA prevents apoptosis by activation of Mek/Erk pathway and requiring Bcl-2. These findings reveal an important genetic and functional relationship between RhoA with Mek/Erk and Bcl-2 for cell survival control during embryogenesis.

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“…ROCK kinases also influence activity of transcription factors (Kuwahara et al, 1999;Yanazume et al, 2002). ROCK kinases are involved in a wide spectrum of cell activities, including cytoskeleton organization, focal adhesion, cell division, cell migration, apoptosis, smooth muscle contraction, and cardiomyocyte hypertrophy (Kuwahara et al, 1999;Amano et al, 2000;Bishop and Hall, 2000;Sah et al, 2000;Aznar and Lacal, 2001;Pruitt and Der, 2001;Yanazume et al, 2002). Recent evidence also suggests that these kinases are involved in cardiac development (Wei et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Rho‐associated kinases play an essential role in cardiac morphogenesis and cardiomyocyte proliferation

Zhao

Rivkees

2002

Developmental Dynamics

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Rho-associated coiled-coil kinases (ROCKs), initially identified as effectors for Rho GTPases, play a role in cardiac cell physiology and are also expressed in the developing heart. However, their role in cardiac development is not known. To investigate the role of these kinases in cardiac development, we examined cardiac development in cultured murine embryos treated with the ROCK inhibitor Y27632. After inhibition of ROCK activity, we found disturbed cardiac chamber formation and trabeculation. To further examine the mechanisms by which ROCK blockade causes cardiac hypoplasia, we assessed programmed cell death and cell proliferation in the hearts. We found decreased cell proliferation in the Y27632-treated hearts, but no changes in programmed cell death. We further observed that ROCK inhibition decreased cardiac myocyte proliferation, suggesting that ROCK kinases regulate cardiomyocyte division. To identify factors involved in ROCK action in regulation of cardiac cell division, we examined expression of cell cycle proteins by using Western blot analysis. We found that ROCK blockade decreased expression of cell cycle proteins, cyclin D3, CDK6, and p27 KIP1 in the hearts and cardiomyocytes, which are required for initiation of cell cycle and G1/S phase transition. These observations show that ROCK kinases play a role in cardiac development and that ROCK kinases regulate cardiac cell proliferation and cell cycle protein expression. Developmental Dynamics 226:24 -32, 2003.

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Rho signals to cell growth and apoptosis

Cited by 286 publications

References 74 publications

Statins induce apoptosis in ovarian cancer cells through activation of JNK and enhancement of Bim expression

Statins induce apoptosis in ovarian cancer cells through activation of JNK and enhancement of Bim expression

RhoA prevents apoptosis during zebrafish embryogenesis through activation of Mek/Erk pathway

Rho‐associated kinases play an essential role in cardiac morphogenesis and cardiomyocyte proliferation

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