RhoA-Binding Kinase α Translocation Is Facilitated by the Collapse of the Vimentin Intermediate Filament Network

Sin, Wun-Chey; Chen, Xiangqun; Leung, Thomas; Lim, Louis

doi:10.1128/mcb.18.11.6325

Cited by 139 publications

(113 citation statements)

References 73 publications

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“…34, No. 6, 2001 Taken together, our results and other accumulating data 14,15,36,37,41,42 raise the question about association of IF with signaling proteins as an important regulator of cell signaling. In this respect, the connection between 14-3-3 and IFs could act as an integrator between structure and signaling.…”

Section: Discussionsupporting

confidence: 57%

Disturbances in hepatic cell-cycle regulation in mice with assembly-deficient keratins 8/18

et al. 2001

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Simple epithelial tissues such as liver and pancreas express keratins 8 (K8) and 18 (K18) as their major intermediate filament proteins. K8 and K18 null mice and transgenic mice that express mutant K18 (K18C) manifest several hepatocyte abnormalities and demonstrate that K8/18 are important in maintaining liver tissue and cell integrity, although other potential functions remain uncharacterized. Here, we report an additional abnormal liver phenotype, which is similar in K8 null, K18 null, and K18C mouse models. Liver histologic examination showed large polynuclear areas that lacked cell membranes, desmosomal structures, and filamentous actin. Similar, but less prominent, areas were observed in the pancreas. The parenchyma outside the polynuclear areas displayed irregular sinusoidal structures and markedly enlarged nuclei. Most K8 null hepatocytes were positive for the proliferating cell nuclear antigen (PCNA) with a doubled DNA content in comparison with the predominantly PCNA-negative wild-type hepatocytes. The distribution of the 14-3-3 protein was also altered in K8 null mice. Taken together, our results indicate that absence of keratin filaments causes disturbances in cellcycle regulation, driving cells into the S-G2 phase and causing aberrant cytokinesis. These effects could stem from disturbed functions of K8/18-dependent cell-cycle regulators, such as the signaling integrator, 14-3-3. (HEPATOLOGY 2001; 34:1174-1183

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Section: Discussionsupporting

confidence: 57%

Disturbances in hepatic cell-cycle regulation in mice with assembly-deficient keratins 8/18

et al. 2001

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“…Previous studies have shown that Rho-kinase is distributed in the cytoplasm (Matsui et al, 1996) but is localized specifi cally at the cleavage furrow during cytokinesis (Kosako et al, 1999) and along actin stress fi bers (Kawabata et al, 2004) and vimentin intermediate fi laments (Sin et al, 1998). EGFP-Rho-kinase, coexpressed with MLC-RFP, distributed diffusely in the cytoplasm and was not found with MLC-RFP at the base of cells within the intact monolayer (Fig.…”

Section: Rho-kinase Accumulates At the Apical-lateral Border Of Cellsmentioning

confidence: 87%

Two distinct modes of myosin assembly and dynamics during epithelial wound closure

Tamada¹,

Perez²,

Nelson³

et al. 2007

The Journal of Cell Biology

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“…The two ROCK isoforms are highly homologous, apart from their PH domains, and act on the same cellular substrates (Yoneda et al 2005(Yoneda et al , 2007. ROCKII recruitment to the membrane is GTP-RhoA dependent (Sin et al 1998) and the PH domain of ROCKII bound PtdIns(3,4,5)P 3 (Yoneda et al 2005). Therefore, membrane recruitment of ROCKII downstream from Rho could depend on localized PtdIns(3,4,5)P 3 enrichment.…”

Section: Contractile Actomyosin Networkmentioning

confidence: 99%

Phosphoinositides in Cell Architecture

Shewan

Eastburn²,

Mostov

2011

Cold Spring Harbor Perspectives in Biology

173

159

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Inositol phospholipids have been implicated in almost all aspects of cellular physiology including spatiotemporal regulation of cellular signaling, acquisition of cellular polarity, specification of membrane identity, cytoskeletal dynamics, and regulation of cellular adhesion, motility, and cytokinesis. In this review, we examine the critical role phosphoinositides play in these processes to execute the establishment and maintenance of cellular architecture. Epithelial tissues perform essential barrier and transport functions in almost all major organs. Key to their development and function is the establishment of epithelial cell polarity. We place a special emphasis on highlighting recent studies demonstrating phosphoinositide regulation of epithelial cell polarity and how individual cells use phosphoinositides to further organize into epithelial tissues.P hosphoinositides (PIs) are essential components of cellular membranes in eukaryotes. Though these specialized lipids comprise less than 1% of the cellular lipid cohort, they play key roles in many fundamental biological processes (Di Paolo and De Camilli 2006;Saarikangas et al. 2010). PIs possess such far ranging roles by serving as specialized membrane docking sites for effectors of numerous cellular signal transduction cascades. PIs also serve as precursors of lipid second messengers. They are concentrated on the cytosolic face of cellular membranes (Fig. 1A) and rapidly diffuse within the plane of the membrane. Reversible phosphorylation of the myo-inositol head group of phosphatidylinositol (PtdIns) at positions 3, 4, and 5 (Fig. 1B) gives rise to the seven PI isoforms identified in eukaryotic cells. PtdIns(4)P and PtdIns(4,5)P 2 are constitutively present in membranes and comprise the largest pool of cellular PIs, whereas PtdIns(3,4,5)P 3 is essentially undetectable in most types of unstimulated cells (Lemmon and Ferguson 2000;Saarikangas et al. 2010).The spatiotemporally regulated production and turnover of phosphoinositides is crucial for localized PI signaling and function. Numerous phosphatidylinositol kinases and phosphatases are involved in regulating the metabolism of the various PI isoforms (Fig. 1)

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RhoA-Binding Kinase α Translocation Is Facilitated by the Collapse of the Vimentin Intermediate Filament Network

Abstract: The regulation of morphological changes in eukaryotic cells is a complex process involving major components of the cytoskeleton including actin microfilaments, microtubules, and intermediate filaments (

Cited by 139 publications

References 73 publications

Disturbances in hepatic cell-cycle regulation in mice with assembly-deficient keratins 8/18

Disturbances in hepatic cell-cycle regulation in mice with assembly-deficient keratins 8/18

Two distinct modes of myosin assembly and dynamics during epithelial wound closure

Phosphoinositides in Cell Architecture

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