Rhodamine 110-Linked Amino Acids and Peptides as Substrates To Measure Caspase Activity upon Apoptosis Induction in Intact Cells

Hug, Hubert; Łos, Marek; Hirt, Werner; Debatin, Klaus‐Michael

doi:10.1021/bi9913395

Cited by 124 publications

(81 citation statements)

References 22 publications

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“…The most recent findings of caspase activity in the marine cyanobacterium Trichodesmium spp. (Berman-Frank et al, 2004) prompted our investigation to utilize caspase activity as a proxy for PCD in M. aeruginosa (Hug et al, 1999, Liu et al, 1999. We demonstrated that caspase activity in stressed M. aeruginosa cells might be responsible for the onset of PCD when induced with H 2 O 2 as a result of oxidative stress (Fig.…”

Section: Discussionmentioning

confidence: 99%

Toxin release in response to oxidative stress and programmed cell death in the cyanobacterium Microcystis aeruginosa

2006

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Section: Discussionmentioning

confidence: 99%

Toxin release in response to oxidative stress and programmed cell death in the cyanobacterium Microcystis aeruginosa

2006

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“…Cells, after 48 h of incubation were rinsed with PBS and incubated with 5 μg/ml D 2 R for 10 min at 37˚C. D 2 R is a non-fluorescent substrate for caspases and is intracellularly cleaved to rhodamine 110 (17). The fluorescence was measured with a confocal microscope Olympus FV500.…”

Section: Methodsmentioning

confidence: 99%

The activity of Selol in multidrug-resistant and sensitive human leukemia cells

Suchocki¹,

Misiewicz²,

Skupińska³

et al. 2007

Oncol Rep

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Abstract. Selol is a mixture of selenitetriglycerides synthesized from sunflower oil. As it contains the element selenium in its structure, it is suspected to exhibit chemopreventive and anticancer activity. In this study, the ability of Selol to inhibit cell proliferation and to induce apoptosis was investigated. Three cell lines were used: leukemia HL-60 cell line and multidrug-resistant HL-60/Dox (resistant to doxorubicin) and HL-60/Vinc (resistant to vincristine). Selol was shown to reduce the cell number as a result of treatment with increasing concentrations. For selected concentrations the evidence of apoptosis (changes in mitochondrial potential and caspase activity) was investigated, as well as changes in lysosome distribution. The study has shown that Selol overcame the cell resistance, as doxorubicin-resistant cells were more sensitive towards Selol than sensitive cells.

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“…To determine caspase-1 activity in intact monocytes, a method described by Hug et al (8) was adapted. PBMC were stimulated with 10 g/ml LPS at 37 or 40°C.…”

Section: Measurement Of Caspase-1 Activity In Monocytesmentioning

confidence: 99%

Febrile Temperatures Attenuate IL-1β Release by Inhibiting Proteolytic Processing of the Proform and Influence Th1/Th2 Balance by Favoring Th2 Cytokines

Boneberg

Hartung

2003

The Journal of Immunology

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We investigated possible feedback mechanisms of febrile temperatures on LPS- and staphylococcal enterotoxin B (SEB)-induced cytokine release in human whole blood. LPS-induced IL-1β release was inhibited at temperatures >38°C, whereas intracellular proIL-1β formation as well as the release of other cytokines except IL-18 were only attenuated above 42°C, indicating that febrile temperatures impair the proteolytic processing of proIL-1β. This attenuated processing is not due to either heat inactivation of caspase-1 or structural changes in proIL-1β produced at higher temperatures. Instead, we propose that febrile conditions change cytosolic compartmentation or trafficking, so that synthesized proIL-1β cannot encounter caspase-1. Febrile temperatures also influenced Th1/Th2 cytokine balance. We observed a 3-fold increase in the Th2-cytokines IL-5 and IL-13 and a reduction to 15% of the Th1-cytokine IL-2 when SEB-stimulated whole blood was incubated at 40°C compared with 37°C. These results indicate that fever limits the production of the fever-inducing IL-1β and also influences the adaptive immune response, favoring Th2 cytokine production.

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Rhodamine 110-Linked Amino Acids and Peptides as Substrates To Measure Caspase Activity upon Apoptosis Induction in Intact Cells

Cited by 124 publications

References 22 publications

Toxin release in response to oxidative stress and programmed cell death in the cyanobacterium Microcystis aeruginosa

Toxin release in response to oxidative stress and programmed cell death in the cyanobacterium Microcystis aeruginosa

The activity of Selol in multidrug-resistant and sensitive human leukemia cells

Febrile Temperatures Attenuate IL-1β Release by Inhibiting Proteolytic Processing of the Proform and Influence Th1/Th2 Balance by Favoring Th2 Cytokines

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