Rhodiola sacra aqueous extract (RSAE) improves biochemical and sperm characteristics in cryopreserved boar semen

Zhao, Hongwei; Li, Q.-w.; Ning, G.-z.; Han, Zengsheng; Jiang, Zhongliang; Duan, Yong-gang

doi:10.1016/j.theriogenology.2008.10.006

Cited by 44 publications

(29 citation statements)

References 42 publications

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“…Zhao et al (2009) reported a direct correlation between the concentration of RSAE and the GSH in sperm cells. They showed that decrease of LPO levels was associated with the increase of the GSH and GPx activity when the freezing medium of boar semen was supplemented with RSAE (Zhao et al, 2009). The main cause of the LPO decrease and GPx increase is unknown, and the active ingredients of ROM and their functional mechanisms on sperm cells organelles are not clear.…”

Section: Discussionmentioning

confidence: 97%

“…However some studies have reported positive effects of ROM and other natural herbs (Rhodiola sacra) using aqueous supplementation prior to cryopreservation for ovine, boar, and canine semen (Zhao et al, 2009;Gil et al, 2010;González et al, 2010;Malo et al, 2010Malo et al, , 2011). In the current study, different combinations of ROM and GSH were applied for bull semen based on the data obtained from previous studies (Zhao et al, 2009;Gil et al, 2010;González et al, 2010;Malo et al, 2010Malo et al, , 2011Gadea et al, 2011;Perumal et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…ved boar spermatozoa (Zhao et al, 2009;Malo et al, 2011). Zhao et al (2009) reported a direct correlation between the concentration of RSAE and the GSH in sperm cells.…”

Section: Discussionmentioning

confidence: 99%

“…ROM has been shown to have beneficial effects on boar (Malo et al, , 2011, canine , and ovine semen freezing. Rhodiola sacra aqueous extract (RSAE) obtained from the roots of the Chinese herb Crassulaceae provides strong scavenging activity against superoxide anion radicals ( Mook-Jung et al, 2002) and can improve lipooxidative and dynamic parameters of cryopreserved boar spermatozoa (Zhao et al, 2009). At present, no studies have evaluated the effect of ROM on freeze-thawed bull semen.…”

mentioning

confidence: 99%

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Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Kia

Olfati-Karaji

Hoseinkhani

et al. 2014

Span. j. agric. res.

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The present study determined the effects of the addition of rosemary extract (ROM), glutathione (GSH), and their combination (ROM + GSH) to freezing extender on the quality of bull semen after cryopreservation. Before cryoperservation, the samples were diluted in a tris-egg yolk (TEY) extender containing 5 mM GSH (treatment I), 5 or 10 g L–1 ROM (treatments II and III), and ROM with GSH (5 mM GSH with 5 or 10 g L–1 of ROM) (treatments IV and V). An extender containing no antioxidants (non-ROM/GSH-treated) served as control group. Kinematic parameters were evaluated by means of a computer-assisted semen analysis (CASA). The viability and membrane integrity of the sperm were assessed using eosin-nigrosin stain and the hypo-osmotic swelling test (HOST) at 0 and 2 h after freezethawing. Lipooxidative parameters, superoxide dismutase, and glutathione peroxidase (GPx) activity were assessed after thawing. Treatment III showed positive effects for total motility (TM) (p < 0.01), average path velocity (VAP) (p < 0.001), viability (p < 0.01) and HOST (p < 0.01); however, lipid peroxidation (LPO) decreased (p < 0.05) and GPx activity increased (p < 0.05) immediately after thawing compared to the control. The TM (p < 0.01), VAP (p < 0.01), viability (p < 0.01), HOST (p < 0.01) decreased in LPO (p < 0.01) and GPx activity (p < 0.05) for treatment V and the viability and GPx activity (p < 0.05) for treatment I were significantly higher than for the control group at 2 h after thawing. It was concluded that the inclusion of ROM and its combination with GSH improves the post-thaw quality of bull semen.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

“…ved boar spermatozoa (Zhao et al, 2009;Malo et al, 2011). Zhao et al (2009) reported a direct correlation between the concentration of RSAE and the GSH in sperm cells.…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Kia

Olfati-Karaji

Hoseinkhani

et al. 2014

Span. j. agric. res.

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“…Zhao et al (2009) observed that Rhodiola sacra extract improved the characteristics of cryopreserved boar spermatozoa by strong sequestering activity against free radicals. In a similar experiment, Malo et al (2010) showed that Rosemary (Rosmarinus officinalis) improved the viability of swine semen and produced better rates of cleavage and blastocysts after the use of semen for in vitro fertilization.…”

Section: Tesmentioning

confidence: 96%

Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm

Reis

Miranda

Silva

et al. 2017

SCA

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Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. Key words: Powdered Coconut Water. Lippia. Cryopreservation. Spermatozoa. Buffalo. ResumoPara a implantação da inseminação artificial é indispensável à utilização de sêmen congelado, que pode provocar mudanças deletérias na estrutura e na integridade das membranas espermáticas, comprometendo sua função. Para evitar estes danos celulares, há a necessidade de se utilizar meios diluidores e substratos adequados que recuperem o maior número possível de células viáveis pós-descongelação. Para isso, foram avaliados, no experimento I, três diferentes diluidores, o diluidor TES-TRIS, bastante utilizado para bubalinos, e um diluidor a base de água de coco em pó (ACP-112  ), associado ou não ao leite (ACP-112  -Leite), na congelação do sêmen de bubalinos; e no experimento II, foi avaliado o efeito do óleo extraído da Lippia origanoides na proteção dos espermatozóides contra as crioinjúrias decorrentes da congelação do sêmen bubalino. Foram utilizados 10 touros bubalinos para as colheitas de sêmen (10 ejaculados/touro), sendo os ejaculados diluídos em TES-TRIS (controle), ACP-112  e ACP-112  -Leite no experimento I; e no experimento II, os ejaculados foram diluídos no melhor diluidor obtido no experimento I, acrescido de 2.5 µg mL -1 , 5 µg mL -1 e 10 µg mL -1 da planta e o grupo controle, constituído somente do diluidor. O sêmen recém colhido foi analisado quanto as características convencionais, tais como, motilidade, concentração, morfologia e viabilidade. Após a descongelação das amostras foram avaliados novamente, motilidade e viabilidade espermática, e posteriormente, foram realizados os testes de termo-resistência, hiposmótico e de avaliação das membranas dos espermatozóides, através das sondas fluorescentes PI, FITC-PSA e JC-1, utilizando a citometria de fluxo. Os dados obtidos foram submetidos à ANOVA e ao Teste de Tukey a 5%. No experimento I, o diluente TES-TRIS apresentou melhores resultados para as várias características avaliadas quando comparado com o ACP-112  e ACP-112  -Leite (P < 0.05), demonstrando maior proteção deste diluidor às estruturas espermáticas durante a criopreservação do sêmen de bubalinos. No experimento II, as diferentes concentrações do óleo extraído da Lippia origanoides não demonstraram nenhuma diferença (P > 0.05) entre as variáveis avaliadas, quanto à proteção das estruturas espermáticas durante a criopreservação. Com base nestes dados, pôde-se demonstrar os efeitos benéficos do diluidor TES-TRIS na qualidade pós-descongelação dos espermatozóides bubalinos; entretanto, nenhum efeito protetor foi observado sobre os espermatozóides bubalinos criopreservados em associação com o óleo extraído da Lippia origanoides, nas diferentes concentrações utilizadas. Palavras-chave: Água de coco em pó. Lippia. Criopreservação. Espermatozóid...

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The role of antioxidants in sperm freezing: a review

et al. 2016

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Cryopreservation of spermatozoa is becoming more important because of new clinical requirements and current clinical practice. Despite the success of sperm cryopreservation this routinely used procedure induces serious detrimental changes in sperm function. Some researchers believe that cryopreservation is associated with DNA fragmentation and DNA single strand breaks in sperm. Mechanisms of cryodamage to human spermatozoa are thought to be multifactorial including: cold shock, osmotic stress, intracellular ice crystal formation, oxidative stress, and combinations of these conditions. Additives showing antioxidative properties reported to reduce the impact of ROS-induced and cold shock damages. Many studies exist as regards the effects of antioxidants on the cryopreservation aimed at improving the quality of post-thaw semen. Hence, this review will clarify results of recent applications of various antioxidants used in numerous research efforts to improve cryopreservation of spermatozoa. This review is to increase the understanding of the roles of these antioxidants concerning mechanisms which enhance resistance to cryodamage of spermatozoa.

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Rhodiola sacra aqueous extract (RSAE) improves biochemical and sperm characteristics in cryopreserved boar semen

Cited by 44 publications

References 42 publications

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm

The role of antioxidants in sperm freezing: a review

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