Rhodobacter thermarum sp. nov., a novel phototrophic bacterium isolated from sediment of a hot spring

Khan, Inam Ullah; Habib, Neeli; Xiao, Min; Li, Mengmeng; Xian, Wen-Dong; Hejazi, Mohammad Saeid; Tarhriz, Vahideh; Zhi, Xiao‐Yang; Li, Wenjun

doi:10.1007/s10482-018-01219-7

Cited by 16 publications

(15 citation statements)

References 34 publications

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“…The cellular fatty acids of strain CYK-10 T were composed of C 18 : 1 ω7c (64.8 %), C 16 : 0 (8.0 %), C 18 : 1 ω7c 11-methyl (5.9 %), C 10 : 0 3-OH (5.8 %), summed feature 3 (C 16 : 1 ω7c and/or C 16 : 1 ω6c; 4.6 %), C 18 : 0 3-OH (4.4 %) and C 18 : 0 (3.4 %) ( Table S2). The major cellular fatty acid of this novel strain was C 18 : 1 ω7c, which is consistent with those reported for the genus Rhodobacter [4,12,[22][23][24].…”

Section: Chemotaxonomysupporting

confidence: 89%

“…Differential characteristics of Rhodobacter tardus CYK-10 T and the type strains of related Rhodobacter species Strains: 1, CYK-10 T ; 2, Rhodobacter thermarum YIM 73036 T ; 3, Rhodobacter flagellatus SYSU G03088 T ; 4, Rhodobacter blasticus ATCC 33485 T ; 5, Rhodobacter capsulatus ATCC 11166 T . Data for R. thermarum YIM 73036 T from Khan et al [22], R. flagellatus SYSU G03088 T and R. blasticus ATCC 33485 T from Xian et al [23] and R. capsulatus ATCC 11166 T from Wang et al [12] and from Subhash and Lee [19]. +, Positive reaction; -, negative reaction.…”

Section: Genome Featuresmentioning

confidence: 99%

“…de/ genus/ rhodobacter). Species of the genus Rhodobacter have been isolated from various environmental sources, including eutrophic freshwater, stagnant water, polluted water, alkaline water, hot spring, lagoon sediment, estuarine water and marine water [15][16][17][18][19][20][21][22][23][24]. Cells are Gram-stain-negative, motile or non-motile and ovoid to rod-shaped.…”

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confidence: 99%

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Tabrizicola oligotrophica sp. nov. and Rhodobacter tardus sp. nov., two new species of bacteria belonging to the family Rhodobacteraceae

Sheu

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-negative, aerobic, non-motile bacteria, designated KMS-5T and CYK-10T, were isolated from freshwater environments. 16S rRNA gene sequence similarity results indicated that these two novel strains belong to the family Rhodobacteraceae . Strain KMS-5T is closely related to species within the genus Tabrizicola (96.1–96.8 % sequence similarity) and Cypionkella (96.5–97.0 %). Strain CYK-10T is closest to Rhodobacter thermarum YIM 73036T with 96.6 % sequence similarity. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set showed that strain KMS-5T is affiliated with species in the genus Tabrizicola and strain CYK-10T is placed in a distinct clade with Rhodobacter blasticus ATCC 33485T, Rhodobacter thermarum YIM 73036T and Rhodobacter flagellatus SYSU G03088T. These two strains shared common chemotaxonomic features comprising Q-10 as the major quinone, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as the principal polar lipids, and C18 : 1 ω7c as the main fatty acid. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between these two novel isolates and their closest relatives were below the cut-off values of 95–96, 90 and 70 %, respectively, used for species demarcation. The obtained polyphasic taxonomic data suggested that strain KMS-5T represents a novel species within the genus Tabrizicola , for which the name Tabrizicola oligotrophica sp. nov. is proposed with KMS-5T (=BCRC 81196T=LMG 31337T) as the type strain, and strain CYK-10T should represent a novel species of the genus Rhodobacter , for which the name Rhodobacter tardus sp. nov. is proposed with CYK-10T (=BCRC 81191T=LMG 31336T) as the type strain.

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Section: Chemotaxonomysupporting

confidence: 89%

Section: Genome Featuresmentioning

confidence: 99%

mentioning

confidence: 99%

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Tabrizicola oligotrophica sp. nov. and Rhodobacter tardus sp. nov., two new species of bacteria belonging to the family Rhodobacteraceae

Sheu

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…The fatty acid compositions of the six strains were similar, with only limited differences in the proportions of some fatty acids. The major cellular fatty acid of these strains was C 18:1 w7c, which is consistent with those reported for the genus Rhodobacter (Khan et al 2019;Pujalte et al 2014;Suresh et al 2020;Wang et al 2014;Xian et al 2020). Strain CCP-1 T exhibited a complex polar lipid pro le consisting of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), two uncharacterized aminophospholipids (APL1 and APL2) and two uncharacterized phospholipids (PL1 and PL2) ( Supplementary Fig.…”

Section: Resultssupporting

confidence: 89%

Rhodobacter ruber sp. nov., isolated from a freshwater pond

Chen

Chang

Sheu

et al. 2021

Preprint

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Strain CCP-1T, isolated from a freshwater pond in Taiwan, is characterized using a polyphasic taxonomy approach. Cells of strain CCP-1T are Gram-stain-negative, aerobic, non-motile, rod-shaped and form dark red colored colonies. Growth occurs at 20–40 oC, at pH 6.5-9 and with 0-0.5% NaCl. Strain CCP-1T contains bacteriochlorophyll a, and shows optimum growth under anaerobic condition by photoheterotrophy, but not by photoautotrophy. 16S rRNA gene sequence similarity indicates that strain CCP-1T is closely related to species within the genus Rhodobacter (93.9–96.2% sequence similarity), Haematobacter (96.3%) and Xinfangfangia (95.5–96.2%). Phylogenetic analyses based on 16S rRNA gene sequences and based on up-to-date bacterial core gene set (92 protein clusters) reveal that strain CCP-1T is affiliated with species in the genus Rhodobacter. The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization identity between strain CCP-1T and Rhodobacter species are 71.3–76.3%, 70.4–77.9% and 21.4–23.2%, respectively, supporting that strain CCP-1T is a novel species of the genus Rhodobacter. The DNA G + C content is 66.2%. The predominant fatty acid is C18:1ω7c and the major isoprenoid quinone is Q-10. The polar lipids have phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two uncharacterized aminophospholipids and two uncharacterized phospholipids. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain CCP-1T should represent a novel species of the genus Rhodobacter, for which the name Rhodobacter ruber sp. nov. is proposed. The type strain is CCP-1T (= BCRC 81189T = LMG 31335T).

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“…Members of this genus are Gram-staining-negative and have an ovoid to rod-shaped morphology [2,13,17]. The chemotaxonomic features include ubiquinone 10 as predominant quinone, C 18 : 0 and C 18 : 1 ω7c branched fatty acids as major fatty acids, and phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) as major polar lipids [2,13,18]. The DNA G+C contents of member of this genus are 62.8-73.2 mol%.…”

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confidence: 99%

Rhodobacter flagellatus sp. nov., a thermophilic bacterium isolated from a hot spring

Xian

Liu

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A thermophilic bacterium, designated SYSU G03088T, was isolated from Moincer hot spring, Tibet, PR China. Polyphasic taxonomic analyses and whole-genome sequencing were used to determine the taxonomic position and genomic profiles of the strain. Phylogenetic analysis using 16S rRNA gene sequence indicated that SYSU G03088T showed highest sequence similarity to Rhodobacter blasticus CGMCC 1.3365T (96.0 % sequence identity). The strain could be differentiated from most recognized species of the genus Rhodobacter by its slightly purple colony colour, distinct phenotypic characters and low ANI values. Cells were Gram-staining negative, and oval-to-rod shaped. Poly-β-hydroxybutyrate and vesicular intracytoplasmic membrane structures were formed inside cells. Growth occurred optimally at 45 °C and pH 7.0. Ubiquinone 10 was the only respiratory quinone. The major fatty acids (>10 %) were C18 : 0, C18 : 1ω7c 11-methyl and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The detected polar lipids of SYSU G03088T included diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The DNA G+C content of SYSU G03088T was 67.7 % (genome). On the basis of the differences in the phenotypic characteristics and phylogenetic analyses, SYSU G03088T is considered to represent a novel species of the genus Rhodobacter , for which the name Rhodobacter flagellatus sp. nov. is proposed. The type strain is SYSU G03088T (=CGMCC 1.16876T=KCTC 72354T).

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Rhodobacter thermarum sp. nov., a novel phototrophic bacterium isolated from sediment of a hot spring

Cited by 16 publications

References 34 publications

Tabrizicola oligotrophica sp. nov. and Rhodobacter tardus sp. nov., two new species of bacteria belonging to the family Rhodobacteraceae

Tabrizicola oligotrophica sp. nov. and Rhodobacter tardus sp. nov., two new species of bacteria belonging to the family Rhodobacteraceae

Rhodobacter ruber sp. nov., isolated from a freshwater pond

Rhodobacter flagellatus sp. nov., a thermophilic bacterium isolated from a hot spring

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