No abstract

A Gram-staining-negative, aerobic, curved rod-shaped and thermophilic bacterial strain, designated YIM 72297T, was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China. Growth was observed at pH 5.0–9.0 with an optimum of pH 7.0–7.5, and at 45–60 °C with an optimum of 55 °C. Positive for catalase and oxidase. The 16S rRNA gene sequence comparison indicated that strain YIM 72297T was most closely related to Elioraea tepidiphila DSM 17972T (96.9 %) and showed <91 % sequence similarities to members of the order Rhodospirillales . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 72297T formed a distinct lineage within the genus Elioraea , and revealed that the genus Elioraea formed a novel family-level clade in the order Rhodospirillales . The ANI and the dDNA-DNA hybridization estimate values between strains YIM 72297T and Elioraea tepidiphila DSM 17972T were 70.8 and 20.1 %, respectively. Strain YIM 72297T contained Q-10 as the predominant ubiquinone. The major fatty acids (>5 %) were summed C18 : 0 (35.8 %), summed feature 8 (30.1 %), C16 : 0 (12.6 %), C18 : 1 2OH (5.6 %) and C16 : 0 2OH (5.4 %). The polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, diphosphosphatidylglycerol and phosphatidylglycerol in addition to two unidentified aminolipids. The DNA G+C content of YIM 72297T was 70.8 mol% (draft genome). On the basis of the polyphasic taxonomic evidence presented in this study, strain YIM 72297T should be classified as representing a novel species of the genus Elioraea , for which the name Elioraea thermophila sp. nov. is proposed, with the type strain YIM 72297T (=CCTCC AB 2017169T=KCTC 62323T). In addition, a novel family, Elioraeaceae fam. nov., is proposed to accommodate the genus Elioraea .

A Gram-negative, non-spore-forming, non-pigmented, strictly aerobic and non-motile short rod bacterium, designated NCCP-1258, was isolated from Cholistan desert soil, Bahawalpur, Pakistan. Growth of strain NCCP-1258 was observed at pH range 6.5-9.5 (optimum 7.5-8.5) and temperature range 20-45 °C (optimum 40 °C), and it tolerated 0-2 % NaCl (optimum 0.5 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain NCCP-1258 belongs to genus Microvirga and is most closely related to Microvirga lotononidis (98.0 %), Microvirga vignae (97.4 %), Microvirga lupini (97.2 %), Microvirga zambiensis (97.2 %) and Microvirga flocculans (97.1 %). Analysis of the concatenated sequences of four housekeeping gene loci (dnaK, gyrB, recA and rpoB) also confirmed the placement of strain NCCP-1258 within the genus Microvirga. DNA-DNA relatedness values of NCCP-1258 with above-mentioned type strains were less than 42 %. The DNA G+C content of strain NCCP-1258 was 64.3 mol%. Chemotaxonomic data (predominant menaquinone system was Q-10; major fatty acids were C, C ω7c and C cyclo ω8c; the polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidyl dimethyl ethanolamine and phosphatidyl ethanolamine) also supported the affiliation of strain NCCP-1258 to the genus Microvirga. On the basis of physiological and biochemical characteristics, phylogenetic analyses and DNA-DNA relatedness, strain NCCP-1258 can be distinguished from the closely related taxa and thus represents a novel species of the genus Microvirga, for which the name Microvirga pakistanensis sp. nov. is proposed with the type strain NCCP-1258 (=CGMCC 1.15074 = KCTC 42496).