Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream

Raj, P. Shalem; Ramaprasad, E. V. V.; Vaseef, S.; Sasikala, Ch.; Ramana, Ch. V.

doi:10.1099/ijs.0.038471-0

Cited by 52 publications

(29 citation statements)

References 32 publications

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“…Separation and identification of lipids was carried out by two-dimensional chromatography on a silica gel TLC plate (Kieselgel 60 F254; Merck) as described earlier (Raj et al, 2013). Phosphatidylethanolamine, phosphatidylglycerol and diphosphatydilglycerol were the major polar lipids of strains JC167…”

Section: Bacillus Subtilis Atcc 6051 T (Aj276351)mentioning

confidence: 99%

Bacillus luteus sp. nov., isolated from soil

Yadav

Sasikala

Ramana

2014

International Journal of Systematic and Evolutionary Microbiology

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Two bacterial strains (JC167 T and JC168) were isolated from a soil sample collected fromMandpam, Tamilnadu, India. Colonies of both strains were orange and cells Gram-stain-positive. Cells were small rods, and formed terminal endospores of ellipsoidal to oval shape. Both strains were positive for catalase, oxidase and hydrolysis of starch/gelatin, and negative for chitin hydrolysis, H 2 S production, indole production and nitrate reduction activity. Major fatty acids of both strains (.5 %) were anteiso-C 15 : 0 , iso-C 16 : 0 , iso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 14 : 0 and C 16 : 0 with minor (,5 but .1 %) amounts of iso-C 17 : 0 , anteiso-C 17 : 0 B/iso-C 17 : 0 I and C 16 : 1 v11c. Diphosphatydilglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell wall amino acids were L-alanine, D-alanine, D-glutamic acid and meso-diaminopimelic acid. b-Carotene and five unidentified carotenoids were present in both strains. Mean genomic DNA G+C content was 53.4±1 mol% and the two strains were closely related (mean DNA-DNA hybridization .90 %). 16S rRNA gene sequence comparisons of both strains indicated that they represent species of the genus Bacillus within the family Bacillaceae of the phylum Firmicutes. Both strains had a sequence similarity of 97.6 % with Bacillus saliphilus 6AG T and ,96.8 % with other members of the genus Bacillus. Sequence similarity between strain JC167 T and 168 was 100 %. Strain JC167 T showed 25.8±1 % reassociation (based on DNA-DNA hybridization) with B. saliphilus DSM 15402 T (56AG T ).Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC167 T as a representative of a novel species of the genus

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Section: Bacillus Subtilis Atcc 6051 T (Aj276351)mentioning

confidence: 99%

Bacillus luteus sp. nov., isolated from soil

Yadav

Sasikala

Ramana

2014

International Journal of Systematic and Evolutionary Microbiology

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“…Members of this genus are Gram-stain-negative and contain bacteriochlorophyll-a. Phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) are the major polar lipids of species of the genus Rhodobacter (Raj et al, 2013). Species of this genus have been isolated from different environmental samples, like a brown-coloured microbial mat (Venkata Ramana et al, 2009), semi-arid tropical soils (Girija et al, 2010), marine habitats (Venkata Ramana et al, 2008;Srinivas et al, 2007), polluted pond sediment (Srinivas et al, 2008) and mud of a stream (Raj et al, 2013).…”

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confidence: 99%

“…Basal medium with sodium pyruvate (0.3 %, w/v), yeast extract (0.3 %, w/v), Casamino acids (0.001 %, w/v) and ammonium chloride (0.01 %, w/v) was used for the growth of both the strains, which were purified on agar slants as described by Raj et al (2013).…”

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Rhodobacter sediminis sp. nov., isolated from lagoon sediments

Yadav

Lee

2016

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-negative, rod-shaped phototrophic bacteria (designated strains N1 T and C7) were isolated from lagoon sediments. Both strains were positive for catalase and oxidase activity. Casein, starch, urea and Tween 20 were hydrolysed by both strains while chitin, gelatin and Tween 80 were not. In both strains, C 16 : 0 , C 18 : 0 ,C 16 : 1 !6c/C 16 : 1 !7c and C 18 : 1 !6c/ C 18 : 1 !7c were the predominant fatty acids, with minor amounts of C 8 : 0 3-OH, anteiso-C 14 : 0 , C 17 : 0 , C 14 : 1 !5c, C 17 : 1 10-methyl and C 18 : 1 !5c. Strains N1 T and C7 contained phosphatidylglycerol and phosphatidylethanolamine as major polar lipids with minor amounts of phosphatidylcholine, unidentified lipids and an unidentified phospholipid. The mean genomic DNA G+C content was 70.6±1 mol% and the two strains were closely related (mean DNA-DNA hybridization >90 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the two strains clustered with species of the genus Rhodobacter belonging to the family Rhodobacteraceae of the class Alphaproteobacteria. Strain N1 T has a 16S rRNA gene sequence similarity of 99.2 % with Rhodobacter capsulatus ATCC 11166 T , 99.1 % with Rhodobacter viridis JA737 T and <96.6 % with other members of the genus Rhodobacter. Strain N1 T and C7 shared 100 % 16S rRNA gene sequence similarity. DNA-DNA hybridization values between strain N1 T and the type strains of the nearest species were clearly below the 70 % threshold. On the basis of phenotypic and genotypic data, it is proposed that strain N1 T represents a novel species of the genus Rhodobacter, for which the name Rhodobacter sediminis sp. nov. is proposed. The type strain is N1 T (=KEMB 563-471 T =JCM 31175 T ), and strain C7 is an additional strain of the species.

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“…We analysed the polar lipids profile by extracting polar lipids with methanol/chloroform/saline (2:1:0.8 by vol) from 1 g freeze-dried cells, as described by Kates et al (1972). Separation and identification of lipids was done by two-dimensional chromatography on a silica gel TLC plate (10 9 10 cm), as described previously (Raj et al 2013). The major quinone of strains PT-11 T and PT-20 T was determined by HPLC after extraction with a chloroform/methanol (2:1 v/v) mixture and purified by TLC (Imhoff 1984;.…”

Section: Morphological Physiological and Chemotaxonomic Testsmentioning

confidence: 99%

Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

Long

Zhang

et al. 2015

Antonie van Leeuwenhoek

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Two moderately halophilic strains, PT-11(T) and PT-20(T), were isolated from saline alkali soil samples collected in Shache County, Xinjiang Province, China. Both strains are aerobic, Gram-positive, motile rods. Strain PT-11(T) grows at 15-40 °C and at pH 6.5-10.0, while PT-20(T) grows at 15-40 °C and at pH 6.5-11.0. The major cellular fatty acids in both strains include anteiso-C15:0, anteiso-C17:0 and iso-C15:0. For both strains, the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and several unidentified lipids. In addition, strain PT-20(T) also contains phosphatidylcholine. The major isoprenoid quinone for both strains is MK-7. The genomic G+C content is 36.7 % for PT-11(T) and 39.2 % for PT-20(T). Phylogenetic analyses of 16S rRNA gene sequences indicated that these two isolates are members of the genus Oceanobacillus. DNA-DNA hybridization indicated that strains PT-11(T) and PT-20(T) should be considered two distinct species. On the basis of both phylogenetic and chemotaxonomic data analyses, therefore, we conclude that PT-11(T) and PT-20(T) represent two novel species within the genus Oceanobacillus, for which we propose the names Oceanobacillus rekensis sp. nov. and Oceanobacillus damuensis sp. nov., respectively. The type strains are PT-11(T) (=KCTC 33144(T) = DSM 26900(T)) and PT-20(T) (=KCTC 33146(T) = DSM 26901(T)).

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Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream

Cited by 52 publications

References 32 publications

Bacillus luteus sp. nov., isolated from soil

Bacillus luteus sp. nov., isolated from soil

Rhodobacter sediminis sp. nov., isolated from lagoon sediments

Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

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