Bacillus luteus sp. nov., isolated from soil

Abstract

Two bacterial strains (JC167 T and JC168) were isolated from a soil sample collected fromMandpam, Tamilnadu, India. Colonies of both strains were orange and cells Gram-stain-positive. Cells were small rods, and formed terminal endospores of ellipsoidal to oval shape. Both strains were positive for catalase, oxidase and hydrolysis of starch/gelatin, and negative for chitin hydrolysis, H 2 S production, indole production and nitrate reduction activity. Major fatty acids of both strains (.5 %) were anteiso-C 15 : 0 , iso-C 16 : 0 , iso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 14 : 0 and C 16 : 0 with minor (,5 but .1 %) amounts of iso-C 17 : 0 , anteiso-C 17 : 0 B/iso-C 17 : 0 I and C 16 : 1 v11c. Diphosphatydilglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell wall amino acids were L-alanine, D-alanine, D-glutamic acid and meso-diaminopimelic acid. b-Carotene and five unidentified carotenoids were present in both strains. Mean genomic DNA G+C content was 53.4±1 mol% and the two strains were closely related (mean DNA-DNA hybridization .90 %). 16S rRNA gene sequence comparisons of both strains indicated that they represent species of the genus Bacillus within the family Bacillaceae of the phylum Firmicutes. Both strains had a sequence similarity of 97.6 % with Bacillus saliphilus 6AG T and ,96.8 % with other members of the genus Bacillus. Sequence similarity between strain JC167 T and 168 was 100 %. Strain JC167 T showed 25.8±1 % reassociation (based on DNA-DNA hybridization) with B. saliphilus DSM 15402 T (56AG T ).Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC167 T as a representative of a novel species of the genus

Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov.

“…These values are slightly higher than that of B. agaradhaerens T (39.3 mol%) (Nielsen et al, 1995), B. neizhouensis JSM 071004 T (40.8 %) (Chen et al, 2009) and B. luteus JC167 T (40.9 %) (Subhash et al, 2014).…”

Bacillus urumqiensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a salt lake

“…T were typical of those observed in members of the genus Bacillus (Carrasco et al, 2007;Subhash et al, 2014;Zhai et al, 2012).…”

“…S2) were diphosphatidylglycerol and phosphatidylglycerol, in accordance with the lipid composition of B. saliphilus 6AG T , 'B. daqingensis' X10-1, B. luteus JC167 T , B. daliensis DLS13 T , B. chagannorensis CG-15 T and the type species, B. subtilis (Kosowski et al, 2014;Subhash et al, 2014;Wang et al, 2014;Zhai et al, 2012). Phosphatidylethanolamine was detected as minor amounts of polar lipid in strain BZ-SZ-XJ18 T ; however, it was abundant in the closely related taxa.…”

“…daqingensis' X10-1 (96.6 % similarity) (Wang et al, 2014), alkaliphile Bacillus luteus JC167 T (96.5 % similarity) (Subhash et al, 2014) T , of the genus Bacillus. The low sequence similarity between strain BZ-SZ-XJ18…”

Physiology and Molecular Phylogeny of Bacteria Isolated from Alkaline Distillery Lime

“…The closest relative of three isolates: H5, H7 and H11 was Bacillus luteus, first isolated from soil sample collected from Mandpam, Tamilnadu, India (Subhash et al, 2014).…”