Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov.

Sultanpuram, Vishnuvardhan Reddy; Mothe, Thirumala

doi:10.1099/ijsem.0.001117

Cited by 34 publications

(12 citation statements)

References 47 publications

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“…The three genera that were not previously detected in human microbiotas ( Alkalibacterium , Chromohalobacter , Salipaludibacillus ) sum up to only <0.01% average relative abundance in the V1V2 dataset. They have been described mainly as environmental high salt tolerant bacteria ( Ventosa et al., 1989 ; Yumoto et al., 2014 ; Sultanpuram and Mothe, 2016 ), indicating they may represent undetected contamination or taxonomic misclassifications.…”

Section: Resultsmentioning

confidence: 99%

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling

Heidrich

Inoue

Asprino

et al. 2022

Front. Cell. Infect. Microbiol.

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Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies. Since most used NGS platforms generate short reads, sequencing the full-length 16S rRNA gene is impractical. Therefore, choosing which 16S rRNA hypervariable region to sequence is critical in microbiota profiling studies. All nine 16S rRNA hypervariable regions are taxonomically informative, but due to variability in profiling performance for specific clades, choosing the ideal 16S rRNA hypervariable region will depend on the bacterial composition of the habitat under study. Recently, NGS allowed the identification of microbes in the urinary tract, and urinary microbiota has become an active research area. However, there is no current study evaluating the performance of different 16S rRNA hypervariable regions for male urinary microbiota profiling. We collected urine samples from male volunteers and profiled their urinary microbiota by sequencing a panel of six amplicons encompassing all nine 16S rRNA hypervariable regions. Systematic comparisons of their performance indicate V1V2 hypervariable regions better assess the taxa commonly present in male urine samples, suggesting V1V2 amplicon sequencing is more suitable for male urinary microbiota profiling. We believe our results will be helpful to guide this crucial methodological choice in future male urinary microbiota studies.

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Section: Resultsmentioning

confidence: 99%

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling

Heidrich

Inoue

Asprino

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The three genera that were not previously detected in human microbiotas (Alkalibacterium, Chromohalobacter, Salipaludibacillus) sum up to only <0.01% average relative abundance in the V1V2 dataset. They have been described mainly as environmental high salt tolerant bacteria (44)(45)(46), indicating they may indeed represent undetected contamination or taxonomic misclassifications.…”

Section: V1v2 Taxonomic Compositionmentioning

confidence: 99%

Choice of 16S ribosomal RNA primers impacts urinary microbiota profiling

Heidrich

Inoue

Asprino

et al. 2022

Preprint

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Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies. Since most used NGS platforms generate short reads, sequencing the full-length 16S rRNA gene is impractical. Therefore, choosing which 16S rRNA hypervariable region to sequence is critical in microbiota profiling studies. All nine 16S rRNA hypervariable regions are taxonomically informative, but due to variability in profiling performance for specific clades, choosing the ideal 16S rRNA hypervariable region will depend on the bacterial composition of the habitat under study. Recently, NGS allowed the identification of microbes in the urinary tract, and urinary microbiota has become an active research area. However, there is no current study evaluating the performance of different 16S rRNA hypervariable regions for urinary microbiota profiling. We collected urine samples from male volunteers and profiled their urinary microbiota by sequencing a panel of six amplicons encompassing all nine 16S rRNA hypervariable regions. After systematically comparing their performance, we show that V1V2 hypervariable regions better assess the taxa commonly present in urine samples and V1V2 amplicon sequencing is more suitable for urinary microbiota profiling. We believe our results will be helpful to guide this crucial methodological choice in future urinary microbiota studies.

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“…S. agaradhaerens was formerly classified as Bacillus agaradhaerens (Nielsen et al 1995). However, it has been recently reclassified as S. agaradhaerens based on chemotaxonomic and biochemical tests (Sultanpuram and Mothe 2016). The genus Salipaludibacillus is a moderate haloalkaliphilic bacterium and included three different species, S. aurantiacus, S. neizhouensis and S. agaradhaerens (Sultanpuram and Mothe 2016).…”

Section: Isolation and Identification Of Cr(vi) Reducing Haloalkaliphilic Bacteriamentioning

confidence: 99%

“…However, it has been recently reclassified as S. agaradhaerens based on chemotaxonomic and biochemical tests (Sultanpuram and Mothe 2016). The genus Salipaludibacillus is a moderate haloalkaliphilic bacterium and included three different species, S. aurantiacus, S. neizhouensis and S. agaradhaerens (Sultanpuram and Mothe 2016). To the best of our knowledge, this is the first report about Cr(VI) bioreduction by S. agaradhaerens.…”

Section: Isolation and Identification Of Cr(vi) Reducing Haloalkaliphilic Bacteriamentioning

confidence: 99%

Bioreduction of hexavalent chromium by a novel haloalkaliphilic Salipaludibacillus agaradhaerens strain NRC-R isolated from hypersaline soda lakes

et al. 2021

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A novel Cr(VI)-resistant haloalkaliphilic bacterial strain NRC-R, identified as Salipaludibacillus agaradhaerens, was isolated from hypersaline soda lakes and characterized for its Cr(VI) bioreduction efficiency. Strain NRC-R grew well and effectively reduced Cr(VI) under a wide range of sodium chloride, pH, shaking velocity and temperature, showing maximum Cr(VI) reduction at 8% NaCl, pH 10, 150 rpm and 35 °C, respectively. Strain NRC-R was able to grow and reduce Cr(VI) effectively in the presence of different heavy metals and oxyanions (Pb 2+ , Zn 2+ , Co 2+ , Mn 2+ , Ni 2+ , Mo 2+ , HPO 4 − , NO 3 − , SO 4 2− and HCO 3 − ). Furthermore, Fe 3+ and Cu 2+ significantly enhanced the Cr(VI) removal by about 1.5 fold. Strain NRC-R could reduce Cr(VI) using a variety of electron donors, exhibiting a maximum reduction in the presence of NADH and fructose. The bioremoval of Cr(VI) using strain NRC-R was due to direct enzymatic reduction and the chromate reductase activity was mainly detected in the bacterial cell membrane. Under the optimized conditions, strain NRC-R showed a remarkable Cr(VI) bioreduction with highest reduction rate of 240 uM/h. Cr(VI) concentrations of up to 3 mM (888.5 mg/L) and 4 mM (1177 mg/L) were completely reduced within 16 h and 32 h, respectively. TEM and SEM-EDX analyses confirmed the biosorption of chromium species into the cells. To the best of our knowledge, this is the first report about Cr(VI) reduction by S. agaradhaerens. In conclusion, S. agaradhaerens NRC-R was a highly efficient Cr(VI) reducing haloalkaliphilic bacterium that has a significant potential in the bioremediation of Cr(VI)-contaminated environments.

show abstract

Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov.

Cited by 34 publications

References 47 publications

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling

Choice of 16S ribosomal RNA primers impacts urinary microbiota profiling

Bioreduction of hexavalent chromium by a novel haloalkaliphilic Salipaludibacillus agaradhaerens strain NRC-R isolated from hypersaline soda lakes

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