Rhodococcus aerolatus sp. nov., isolated from subarctic rainwater

Lee

International Journal of Systematic and Evolutionary Microbiology

et al. 2016

T grew optimally at 25 C and pH 7.5-8.5 in the presence of 1.0-3.0 % (w/v) sea salts. The major cellular fatty acids (>10 %) were C 16 : 1 !6c and/or C 16 : 1 !7c, C 16 : 0 , and C 18 : 1 !6c and/or C 18 : 1 !7c. The genomic DNA G+C content was 39.7 mol%. On the basis of the phylogenetic, genomic, chemotaxonomic and phenotypic data presented, we propose the name Pseudoalteromonas neustonica sp. nov. with the type strain PAMC 28425 T (=KCCM 43187 T =JCM 31286 T ).The genus Pseudoalteromonas was established by recombining 11 species of the genus Alteromonas and 1 species of the genus Pseudomonas into 12 species of the genus Pseudoalteromonas based on a wealth of phylogenetic analyses of nearly complete 16S rRNA gene sequences (Gauthier et al., 1995). The genus Pseudoalteromonas is classified in the family Alteromonadaceae, and members of this genus are Gramstain negative, aerobic, motile and have C 16 : 0 , C 16 : 1 !7c, C 17 : 1 !8c and C 18 : 1 !7c as major fatty acids and genomic G +C contents of 38-48 mol% (Bowman & McMeekin, 2005). At the time of writing, the genus Pseudoalteromonas comprises 41 species and 2 subspecies with validly published names (List of Prokaryotic Names with Standing in Nomenclature, http://www.bacterio.net/pseudoalteromonas.html; Park et al., 2016;Ying et al., 2016). Most species of the genus Pseudoalteromonas have been isolated from various marine habitats including seawater, sediment, marine invertebrates, algae and sea ice (Bowman & McMeekin, 2005). With cultivation-independent molecular techniques, pseudoalteromonads are also known as a ubiquitous marine group extending from the sea surface microlayer (SSM) to deep-sea sediment, and from equatorial to polar regions (Cui et al., 2008;Cunliffe & Murrell, 2009;Wietz et al., 2010).Here, we isolated a bacterial strain, which is the first representative of the genus Pseudoalteromonas isolated from Antarctic SSM to our knowledge, and performed a polyphasic taxonomic analysis to determine the taxonomic position of the strain.A SSM sample was taken using a customized SSM sampler employing a type of rotating drum (Harvey 1966) covered with polycarbonate from the Terra Nova Bay (75.29 S Abbreviations: ANI, average nucleotide identity; GGDC, genome-togenome distance calculation; SSM, sea surface microlayer.The GenBank/EMBL/DBBJ accession number for the 16S rRNA gene sequence and the draft genome sequence of strain PAMC 28425 T are KU716039 and BDDS01000000, respectively.Two supplementary tables and one supplementary figure are available with the online Supplementary Material.

Section: Phenotypic Characteristics Of Strain Pamc 28425mentioning

confidence: 99%

“…Direct sequencing of purified PCR products for the 16S rRNA gene was performed as described by Hwang et al (2015). The nearly complete 16S rRNA gene sequence (1418 bp) of strain PAMC 28425 T was obtained and analysed using BLAST search against the GenBank and EzTaxone databases (Altschul et al, 1990;Kim et al, 2012).…”

mentioning

confidence: 99%

Pseudoalteromonas neustonica sp. nov., isolated from the sea surface microlayer of the Ross Sea (Antarctica), and emended description of the genus Pseudoalteromonas

Lee

International Journal of Systematic and Evolutionary Microbiology

et al. 2016

“…Corynebacterium tuberculostearicum (Brown et al 1984), Rhodococcus ruber (Hwang et al 2015), and Nocardia donostiensis (Ercibengoa et al 2016). This information may suggest that a system was adopted in nature to avoid the toxicity of 19:1∆Me10 by reducing it to 19:0Me10.…”

Section: Effect Of Synthesizing 10-methylene Stearic Acid (19:1∆me10)mentioning

confidence: 99%

Characterization of cyanobacterial cells synthesizing 10-methyl stearic acid

Machida

Suzuki

2018

Photosynth Res

Recently, microalgae have attracted attention as sources of biomass energy. However, fatty acids from the microalgae are mainly unsaturated and show low stability in oxygenated environments, due to oxidation of the double bonds. The branched-chain fatty acid, 10-methyl stearic acid, is synthesized from oleic acid in certain bacteria; the fatty acid is saturated, but melting point is low. Thus, it is stable in the presence of oxygen and is highly fluid. We previously demonstrated that BfaA and BfaB in Mycobacterium chlorophenolicum are involved in the synthesis of 10-methyl stearic acid from oleic acid. In this study, as a consequence of the introduction of bfaA and bfaB into the cyanobacterium, Synechocystis sp. PCC 6803, we succeeded in producing 10-methyl stearic acid, with yields up to 4.1% of the total fatty acid content. The synthesis of 10-methyl stearic acid in Synechocystis cells did not show a significant effect on photosynthetic activity, but the growth of the cells was retarded at 34 °C. We observed that the synthesis of 10-methylene stearic acid, a precursor of 10-methyl stearic acid, had an inhibitory effect on the growth of the transformants, which was mitigated under microoxic conditions. Eventually, the amount of 10-methyl stearic acid present in the sulfoquinovosyl diacylglycerol and phosphatidylglycerol of the transformants was remarkably higher than that in the monogalactosyldiacylglycerol and digalactosyldiacylglycerol. Overall, we successfully synthesized 10-methyl stearic acid in the phototroph, Synechocystis, demonstrating that it is possible to synthesize unique modified fatty acids via photosynthesis that are not naturally produced in photosynthetic organisms.

“…PCR amplification of the 16S rRNA gene and direct sequencing of purified PCR products were performed as described by Hwang et al (2015). The almost-complete 16S rRNA gene sequence (1474 bp) of strain PAMC 27536…”

mentioning

confidence: 99%

Marinobacterium profundum sp. nov., a marine bacterium from deep-sea sediment

International Journal of Systematic and Evolutionary Microbiology

Yoon

Lee

et al. 2016

A Gram-stain-negative, rod-shaped and motile strain, designated PAMC 27536 T , was isolated from deep-sea sediment in the East Sea, Korea. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Marinobacterium. Phylogenetic analyses revealed that strain PAMC 27536T was related most closely to Marinobacterium rhizophilum CL-YJ9T with a 16S rRNA gene sequence similarity of 98.5 % and to other members of the genus Marinobacterium (94.0-91.7 %). Genomic relatedness analyses between strain PAMC 27536 T and M. rhizophilum KCCM 42386 T gave an average nucleotide identity of 85.6 % and an estimated DNA-DNA hybridization of 24.6 % using the genome-to-genome distance calculator, indicating that they represent genomically distinct species. Cells of strain PAMC 27536 T grew optimally at 25-30 8C and pH 7.0-7.5 in the presence of 3 % (w/v) sea salts. The major cellular fatty acids were C 16 : 1 v6c and/or C 16 : 1 v7c, C 18 : 1 v6c and/or C 18 : 1 v7c, and C 16 : 0 . The major isoprenoid quinone was Q-8. The genomic DNA G+C content was 56.1-57.2 mol%. Based on the phylogenetic, chemotaxonomic, genomic and phenotypic data presented, a novel species with the name Marinobacterium profundum sp. nov. is proposed, with PAMC 27536 T (5KCCM 43095 T 5JCM 30410 T ) as the type strain.