Rhododendrin ameliorates skin inflammation through inhibition of NF-κB, MAPK, and PI3K/Akt signaling

Jeon, Yoon-Jae; Kim, Byung-Hak; Kim, Sunghwan; Oh, Ikhoon; Lee, Sooryun; Shin, Jongheon; Kim, Taeyoon

doi:10.1016/j.ejphar.2013.05.041

Cited by 42 publications

(36 citation statements)

References 55 publications

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“…20 Briefly, a crude extract was prepared from powdered leaves of R. brachycarpum by dichloromethane and methanol treatment. The extract was then partitioned between water (H 2 O) and n -butanol, followed by partitioning in n -hexane and 15% aqueous methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Rhododendrin-treated keratinocytes showed no toxic effects at rhododendrin concentrations of up to 300 μ M and exhibited more than 90% cell viability, indicating that the compound is not cytotoxic. 20 …”

Section: Methodsmentioning

confidence: 99%

“…16, 17, 18 Among these natural products, rhododendrin extracted from the genus Rhododendron has been used in traditional Oriental medicine to treat inflammatory diseases of the skin and has been shown to have a strong anti-inflammatory effect. 19, 20 However, its precise mechanism of action is not clearly understood. Here, we demonstrate that rhododendrin isolated from Rhododendron brachycarpum leaves exerts significant anti-inflammatory effects in IMQ-induced psoriasis-like skin inflammation in mice and investigate the basic regulatory mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Rhododendrin inhibits toll-like receptor-7-mediated psoriasis-like skin inflammation in mice

et al. 2017

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Many active compounds present in Rhododendron brachycarpum have been used in traditional Oriental medicine for the treatment of various skin diseases. However, the precise mechanism of action of the compounds isolated from R. brachycarpum and their relevance as therapeutics for the treatment of psoriasis remain elusive. In this study, we report that rhododendrin isolated from R. brachycarpum strongly inhibits imiquimod (IMQ)-induced psoriasis-like skin inflammation in mice. We showed that topical treatment with rhododendrin reduces IMQ-induced skin hyperplasia, inflammatory mononuclear cell infiltration and the expression of pro-inflammatory mediators in mouse skin. In addition, we found that rhododendrin inhibits the activation of the TLR-7/NF-κB and mitogen-activated protein kinase pathways in both IMQ-induced psoriasis-like skin inflammation in mice and in normal human epidermal keratinocytes treated with IMQ. These results suggest that rhododendrin has an anti-inflammatory effect and can be used as a therapeutic to fight against psoriasis and other inflammatory skin diseases.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rhododendrin inhibits toll-like receptor-7-mediated psoriasis-like skin inflammation in mice

et al. 2017

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“…The GSH redox cycle represents the most important antioxidant system in endothelial cells, and GSH depletion has been shown to occur in many cardiovascular diseases, including atherosclerosis 10) . The current study showed that luteolin is able to partly reverse the effects of TNF-α on the level of GSH and activity of SOD in HUVECs.…”

Section: Discussionmentioning

confidence: 99%

“…[7][8][9] . TNF-α is a multifunctional cytokine that is believed to mediate the inflammatory response by activating associated signaling pathways, including the mitogen-activated protein kinase (MAPK) pathway and the transcription of NF-κB 10) . Moreover, TNF-α induces the activation of other cytokines, including TNF-α itself, as well as increased ROS production, which may result in a vicious circle, thus aggravating inflammatory processes 11) .…”

Section: Ldh Gsh and Sod Assaymentioning

confidence: 99%

Luteolin Protects HUVECs from TNF-α-induced Oxidative Stress and Inflammation via its Effects on the Nox4/ROS-NF-κB and MAPK Pathways

Xia

Wang

Jin

et al. 2014

JAT

131

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Aim: Inflammation and oxidative stress are now recognized to be two important contributing factors to the development of atherosclerosis (AS). NADPH oxidase-4 (Nox4)-derived reactive oxygen species (ROS), NF-κB and MAPK play crucial roles in these processes. Luteolin, a flavone rich in many plants, can interrupt the molecular expression and inhibit the progression of inflammation and oxidative stress. The present study was designed to test whether luteolin inhibits TNF-α-induced inflammation and oxidative stress in human umbilical vein endothelial cells (HUVECs) and identify some of the mechanisms underlying these effects.

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Chemical composition of tetraploid Gynostemma pentaphyllum gypenosides and their suppression on inflammatory response by NF‐κB/MAPKs/AP‐1 signaling pathways

Wang

Gao

et al. 2020

Food Science & Nutrition

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The chemical composition and anti‐inflammatory activity of gypenosides isolated from tetraploid Gynostemma pentaphyllum (GP) leaves were investigated. The gypenosides accounted for 7.43 mg/g of the tested GP sample, which were composed of four major saponins including isomers of gypenoside 1 and 2 (C47H76O18), 3 (C47H76O17), and 4 (C46H74O17). Pretreatment of gypenosides reduced mRNA expressions of the proinflammatory mediators in LPS‐stimulated RAW264.7 macrophage cells, such as IL‐6, IL‐1β, COX‐2, and TNF‐α in a dose‐dependent manner. The secreted protein levels of IL‐6 and TNF‐α, and NO production were also decreased by gypenosides within the concentration range of 50–200 μg/ml. Moreover, the mechanism studies demonstrated that gypenosides (200 μg/ml) treatment significantly inhibited the nuclear translocation of nuclear factor‐κB and activator protein 1 (c‐Fos and c‐Jun) through down‐regulating the phosphorylation of their upstream IκB kinase and mitogen‐activated protein kinases (MAPKs), especially that of c‐Jun N‐terminal kinase and extracellular regulated protein kinase(JNK and ERK), but not that of the p38 MAPK. These results suggested that the gypenosides might have potential anti‐inflammatory effect and use for improving human health.

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Rhododendrin ameliorates skin inflammation through inhibition of NF-κB, MAPK, and PI3K/Akt signaling

Cited by 42 publications

References 55 publications

Rhododendrin inhibits toll-like receptor-7-mediated psoriasis-like skin inflammation in mice

Rhododendrin inhibits toll-like receptor-7-mediated psoriasis-like skin inflammation in mice

Luteolin Protects HUVECs from TNF-α-induced Oxidative Stress and Inflammation via its Effects on the Nox4/ROS-NF-κB and MAPK Pathways

Chemical composition of tetraploid Gynostemma pentaphyllum gypenosides and their suppression on inflammatory response by NF‐κB/MAPKs/AP‐1 signaling pathways

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