Rhodohalobacter sulfatireducens sp. nov., isolated from a marine solar saltern

Abstract

A novel Gram-stain-negative, oxidase-positive, catalase-positive, non-motile, facultatively anaerobic, and rod-shaped bacterium, designated WB101 T , was isolated from a marine solar saltern located in Wendeng, PR China. Growth occurs at 25-42°C (optimum, 35-37°C), 1.0-11.0% (w/v) NaCl (optimum, 5.0-6.0%), and pH 7. 0-8.5 (optimum, 7.5-8.0). Analysis based on the 16S rRNA gene sequences revealed that WB101 T shared high level of sequence similarity with Rhodohalobacter barkolensis 15182 T (93.5%), Rhodohalobacter halophilus JZ3C29 T (93.2%), and 'Rhodohalobacter mucosus' 8A47 T (92.1%). Strain WB101 T formed an evident species-level clade within the genus Rhodohalobacter in both phylogenetic and phylogenomic topologies. The draft genome of strain WB101 T is 5,104,032 bp in size, and the G + C content is 42.0 mol%. The genomic sequencing yields 138 contigs with an N50 value of 123,641 and a coverage depth of 275.0 ×. The strain encodes a complete assimilatory sulfate reduction pathway. The sole respiratory quinone was menaquinone 7. The dominant cellular fatty acid (≥ 10%) was iso-C 15:0 . The polar lipid pro le of the novel isolate included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylethanolamine. Based on its phylogenetic, physiological, and biochemical properties, strain WB101 T should be classi ed as a novel species of the genus Rhodohalobacter, for which the name Rhodohalobacter sulfatireducens sp. nov. is proposed. The type strain is WB101 T (= KCTC 92204 T = MCCC 1H00518 T ).