Two novel rod-shaped and Gram-negative bacterial strains, designated A6E486T and 5E331T, were isolated from a coastal sediment sample taken from Xiaoshi Island, Weihai, China, and a fresh kelp sample collected from a kelp culture area, Rongcheng, China, respectively. Growth of strain A6E486T occurred at 20°C–43°C (optimum, 33°C–35°C) at pH 5.5–7.5 (optimum, 6.5) and in the presence of 1.0%–5.5% (w/v) NaCl (optimum, 2.5%–3.0%). Strain 5E331T grew with 1.5%–5.0% (w/v) NaCl (optimum, 3.0%) at 15°C–40°C (optimum, 33°) and pH 6.0–8.5 (optimum, 7.0). The similarity of 16S rRNA gene sequence between the two strains was 95.2%. The phylogenetic analysis based on 16S rRNA gene sequence showed that strains A6E486T and 5E331T belong to the genus Kordiimonas, sharing the highest similarity to the genus Kordiimonas (94.6%–96.8%, 94.9%–96.1%, respectively). Strains A6E486T and 5E331T had percentage of conserved protein (POCP) values of 56.0%–67.3% and average nucleotide identity (ANI) values of 68.8%–73.1% to members of the genus Kordiimonas. The major polar lipids detected in the two strains were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and unidentified glycolipids, aminolipids, and lipids. The predominant respiratory quinone of the two strains was Q-10. Based upon the results presented in this study, strains A6E486T and 5E331T represent two novel species of the genus Kordiimonas, for which the names Kordiimonas marina and Kordiimonas laminariae are proposed with the type strains A6E486T (= KCTC 82758T = MCCC 1H00470T) and 5E331T (= KCTC 92199T = MCCC 1H00515T), respectively. Comparative genomic analysis showed that seven species of the genus Kordiimonas shared 1,258 core genes and had differences in carbohydrate metabolism, energy metabolism, and cofactor and vitamin metabolism. The pan-genome of the genus Kordiimonas was open. The prediction of secondary metabolites showed that most strains of the genus Kordiimonas had the ability to produce homoserine lactones, one of the most important signal molecules in the quorum-sensing system of Gram-negative bacteria. Additionally, numerous genes involved in bacterial defense, motility and chemotaxis, cold adaptation, and environment stress response were found in the genus Kordiimonas, indicating the marine-adapted lifestyle of members of the genus Kordiimonas.

A Gram-stain-negative, rod-shaped, glide, non-flagellated, and facultatively aerobic bacterial strain, designated as Z654T, was isolated from the gut of abalone Haliotis discus hannai from Rongcheng, Shandong province, China. Cells are 0.2–0.8 µm in width and 0.7–3.4 µm in length. Cells grew best at 30°C (range, 15–37°C), pH 7 (range, 6.0–8.5) and NaCl concentration of 2.0% (w/v) (range, 1–10%). According to the phylogenetic analysis of 16S rRNA gene sequence, the strain belongs to the genus Halocynthiibacter and the most close strain is Halocynthiibacter arcticus KCTC 42129T (97.12%). The genome size of strain Z654T was 3296250 bp and the DNA G + C content was 54.2 mol%. The average nucleotide identity (ANI) scores and digital DNA–DNA hybridization (dDDH) scores with H. arcticus KCTC 42129T was 70% and 14.6–18.2% respectively. The predominant quinone was Q-10 and the major fatty acids were C18:0, C18:1 ω7c 11-methyl and summed future 8. The polar lipids were consisted of phosphatidylcholine, phosphatidylglycerol, unidentified aminolipid and unidentifed lipids. Based on the phenotypic, phylogenetic and chemotaxonomic data, strain Z654T was considered to represent a novel species of the genus Halocynthiibacter, for which the name Halocynthiibacte halioticoli sp. nov., is proposed. The type strain is Z654T (= MCCC 1H00503T = KCTC 92003T).

A novel Gram-stain-negative, oxidase-positive, catalase-positive, non-motile, facultatively anaerobic, and rod-shaped bacterium, designated WB101 T , was isolated from a marine solar saltern located in Wendeng, PR China. Growth occurs at 25-42°C (optimum, 35-37°C), 1.0-11.0% (w/v) NaCl (optimum, 5.0-6.0%), and pH 7. 0-8.5 (optimum, 7.5-8.0). Analysis based on the 16S rRNA gene sequences revealed that WB101 T shared high level of sequence similarity with Rhodohalobacter barkolensis 15182 T (93.5%), Rhodohalobacter halophilus JZ3C29 T (93.2%), and 'Rhodohalobacter mucosus' 8A47 T (92.1%). Strain WB101 T formed an evident species-level clade within the genus Rhodohalobacter in both phylogenetic and phylogenomic topologies. The draft genome of strain WB101 T is 5,104,032 bp in size, and the G + C content is 42.0 mol%. The genomic sequencing yields 138 contigs with an N50 value of 123,641 and a coverage depth of 275.0 ×. The strain encodes a complete assimilatory sulfate reduction pathway. The sole respiratory quinone was menaquinone 7. The dominant cellular fatty acid (≥ 10%) was iso-C 15:0 . The polar lipid pro le of the novel isolate included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylethanolamine. Based on its phylogenetic, physiological, and biochemical properties, strain WB101 T should be classi ed as a novel species of the genus Rhodohalobacter, for which the name Rhodohalobacter sulfatireducens sp. nov. is proposed. The type strain is WB101 T (= KCTC 92204 T = MCCC 1H00518 T ).

A Gram-stain-negative, rod-shaped, glide, non-flagellated, and facultatively aerobic bacterial strain, designated as Z654T, was isolated from the gut of abalone Haliotis discus hannai from Rongcheng, Shandong province, China. Cells are 0.2–0.8 µm in width and 0.7–3.4 µm in length. Cells grew best at 30°C (range, 15–37°C), pH 7 (range, 6.0–8.5) and NaCl concentration of 2.0% (w/v) (range, 1–10%). According to the phylogenetic analysis of 16S rRNA gene sequence, the strain belongs to the genus Halocynthiibacter and the most close strain is Halocynthiibacter arcticus KCTC 42129T (97.12%). The genome size of strain Z654T was 3296250 bp and the DNA G + C content was 54.2 mol%. The average nucleotide identity (ANI) scores and digital DNA–DNA hybridization (dDDH) scores with H. arcticus KCTC 42129T was 70% and 14.6–18.2% respectively. The predominant quinone was Q-10 and the major fatty acids were C18:0, C18:1 ω7c 11-methyl and summed future 8. The polar lipids were consisted of phosphatidylcholine, phosphatidylglycerol, unidentified aminolipid and unidentifed lipids. Based on the phenotypic, phylogenetic and chemotaxonomic data, strain Z654T was considered to represent a novel species of the genus Halocynthiibacter, for which the name Halocynthiibacte halioticoli sp. nov., is proposed. The type strain is Z654T (= MCCC 1H00503T = KCTC 92003T).

A novel Gram-stain-negative, oxidase-positive, catalase-positive, non-motile, facultatively anaerobic, and rod-shaped bacterium, designated WB101T, was isolated from a marine solar saltern located in Wendeng, PR China. Growth occurs at 25–42°C (optimum, 35–37°C), 1.0–11.0% (w/v) NaCl (optimum, 5.0–6.0%), and pH 7.0–8.5 (optimum, 7.5–8.0). Analysis based on the 16S rRNA gene sequences revealed that WB101T shared high level of sequence similarity with Rhodohalobacter barkolensis 15182T (93.5%), Rhodohalobacter halophilus JZ3C29T (93.2%), and ‘Rhodohalobacter mucosus’ 8A47T (92.1%). Strain WB101T formed an evident species-level clade within the genus Rhodohalobacter in both phylogenetic and phylogenomic topologies. The draft genome of strain WB101T is 5,104,032 bp in size, and the G + C content is 42.0 mol%. The genomic sequencing yields 138 contigs with an N50 value of 123,641 and a coverage depth of 275.0 ×. The strain encodes a complete assimilatory sulfate reduction pathway. The sole respiratory quinone was menaquinone 7. The dominant cellular fatty acid (≥ 10%) was iso-C15:0. The polar lipid profile of the novel isolate included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylethanolamine. Based on its phylogenetic, physiological, and biochemical properties, strain WB101T should be classified as a novel species of the genus Rhodohalobacter, for which the name Rhodohalobacter sulfatireducens sp. nov. is proposed. The type strain is WB101T (= KCTC 92204T = MCCC 1H00518T).