Rhodomicrobium Vannielii, a New Photoheterotrophic Bacterium

Duchow, Esther; Douglas, H. C.

doi:10.1128/jb.58.4.409-416.1949

Cited by 98 publications

(34 citation statements)

References 7 publications

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“…AND METtIODS a) Cultures: The culture of R. vannidii used was strain 450 from the departmental collection, and the culture of Rhodospirillum rubrum was supplied by Dr. R. S. Wolfe. A single medium (5) was adequate for the growth of both organisms, but the yield of R. rubrum was greatly improved by a fourfold increase in the concentration of yeast extract. The bulk medium was inoculated in a ratio of 1 ml to 150 ml of medium and transferred to screw-capped tubes 6…”

Section: Methodsmentioning

confidence: 99%

FINE STRUCTURE OF THE PHOTOSYNTHETIC BACTERIUM RHODOMICROBIUM VANNIELII

Boatman

Douglas

1961

The Journal of Cell Biology

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The fine structure of the photosynthetic bacterium Rhodomicrobium vannielii was studied by the ultra thin scctioning technique. Cclls were fixed in buffered osmium tctroxide and embedded in Epoxy resin. The feature most common to nearly all cells was an array of intracellular membranes situated in a concentric manner at the periphery of the cell. The membranes were mostly paired and quite often five pairs were seen aligned together. Calculations from densitomctric tracings showed the average width of a "unit" membrane to be 65 A. Sections of material from disrupted cells after passage through a sucrose gradient revcaled vesicular forms composed of membranes similar in width to those in the intact cell. Absorption spectra of both intact cells and isolated membrancs were very similar in the bacteriochlorophyll regions. Septa and membranes were demonstrated in the filaments that join mature cells. No evidence for chromatophores was obtained although the methods used were adequate for their demonstration in Rhodospirillum rubrum.

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Section: Methodsmentioning

confidence: 99%

FINE STRUCTURE OF THE PHOTOSYNTHETIC BACTERIUM RHODOMICROBIUM VANNIELII

Boatman

Douglas

1961

The Journal of Cell Biology

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“…We anticipate that future insights into bacterial reproduction mechanisms will help clarify any 265 connections between ICMs and cell growth and division. Separately, because ICMs are 266 conditionally made in PNSB [14][15][16] , ICM localization patterns could differentially impact population 267 fitness during shifts between phototrophic and non-phototrophic (e.g., aerobic) conditions. For Bacteriochlorophyll (BChl) a was extracted and quantified as previously described 14,52 .…”

Section: Discussion 230mentioning

confidence: 99%

“…Second, BChl is an obligate 79 component of PNSB photosystems and is thus essential for ICM synthesis 3 . Finally, BChl exists 80 almost entirely within photosystems, with very little free in the cytoplasm 22 , and these 81 photosystems predominantly reside within ICMs 7,15,23,24 . 82…”

Section: Introduction 43mentioning

confidence: 99%

“…ICM architectures are 53 currently understood to result from inter-molecular interactions between components of the 54 photosynthetic machinery [10][11][12][13] . 55Most PNSB synthesize ICMs only under low-oxygen conditions that allow anoxygenic 56 photosynthesis [14][15][16] ; cells grown anaerobically in light (phototrophically) contain ICMs, whereas 57 those grown aerobically in darkness (chemotrophically) do not. ICMs have been used as model 58 systems for studying membrane biogenesis and photochemistry 2,3,17 , but remain poorly 59 characterized with regard to cellular organization, presumably because studies relied on 60 biochemical analysis of bulk ICM and electron microscopy of a small number of cells.…”

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Diverse spatial organization of photosynthetic membranes among purple nonsulfur bacteria

LaSarre

Kysela

Stein

et al. 2018

Preprint

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24In diverse bacteria, proper cellular physiology requires the utilization of protein-or membrane-25 bound compartments that afford specific metabolic capabilities. One such compartment is the 26 light-harvesting intracytoplasmic membrane (ICM) of purple nonsulfur bacteria (PNSB). Here we 27 reveal that ICMs are subject to differential spatial organization among PNSB. We visualized 28ICMs in live cells of fourteen PNSB species by exploiting the natural autofluorescence of the 29 photosynthetic machinery. We then quantitatively characterized ICM localization using 30 automated computational analysis of autofluorescence patterns within single cells across the 31 population. Our studies revealed that ICMs are localized in distinct subcellular patterns that 32 differ between species; some PNSB elaborate ICMs throughout the cell, while others spatially 33 restrict ICM to varying degrees. The most highly-restricted ICMs were localized in a specific 34 pattern corresponding to progression of cell growth and division. An identical pattern of ICM 35 restriction was conserved across at least two genera. Phylogenetic and phenotypic comparisons 36 established that ICM localization and ICM architecture are not strictly interdependent and that 37 neither trait fully correlates with the evolutionary relatedness of the species. This discovery of 38 new diversity in bacterial cell organization has implications for understanding both the 39 mechanisms underpinning spatial arrangement of bacterial compartments and the potential 40

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“…Since the first isolation of a prosthecate, budding bacterium, Hyphomicrobium vulgare [1], an abundance of morphologically similar microorganisms has been found. These have been placed into the genera Hyphomicrobium [2][3][4][5][6], Rhodornicrobium [7], Hyphomonas [8], and Pedomicrobiurn [9,10].…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

DNA-DNA reassociation studies on the genus Pedomicrobium

Gebers

1981

FEMS Microbiology Letters

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Rhodomicrobium Vannielii, a New Photoheterotrophic Bacterium

Cited by 98 publications

References 7 publications

FINE STRUCTURE OF THE PHOTOSYNTHETIC BACTERIUM RHODOMICROBIUM VANNIELII

FINE STRUCTURE OF THE PHOTOSYNTHETIC BACTERIUM RHODOMICROBIUM VANNIELII

Diverse spatial organization of photosynthetic membranes among purple nonsulfur bacteria

DNA-DNA reassociation studies on the genus Pedomicrobium

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