Rhodopsin, light-sensor of vision

Hofmann, Klaus Peter; Lamb, Trevor D

doi:10.1016/j.preteyeres.2022.101116

Cited by 45 publications

(40 citation statements)

References 557 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This spectral identification of NAD(P)H is in agreement with the CV measurements (Figure b). Rod photoreceptors, native to the retina and present in RO, respond to distinct wavelengths of light (∼500 nm) and initiate a cascade of events that result in information transfer to the brain . We wished to assess whether an RO can produce photo-responsive electrical currents in the dark and in the light.…”

Section: Resultsmentioning

confidence: 99%

Mammalian Fuel Cells Produce Electric Current

Shlosberg

Faynus

Huang

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The increasing concern about climate change has led scientists around the world to develop clean energy technologies that may replace the traditional use of fossil fuels. A promising approach is the utilization of unicellular organisms as electron donors in bio-fuel cells. To date, this method has been limited to microorganisms such as bacteria, yeast, and microalgae. In this work, we show for the first time the concept of using mammalian cell cultures and organoids as electron donors in biofuel cells. We apply cyclic voltammetry to show that upon association of ARPE19 cells with the anode, they release reducing molecules to produce electricity. Furthermore, we apply 2D-fluorescence measurements and show that upon illumination, photosensitive stem cell-derived retinal organoids, which consist of rod photoreceptors and interneurons, secrete NADH and NADPH molecules that can donate electrons at the anode to produce photocurrent.

show abstract

Section: Resultsmentioning

confidence: 99%

Mammalian Fuel Cells Produce Electric Current

Shlosberg

Faynus

Huang

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Nonadiabatic processes, in which more than one Born–Oppenheimer potential energy surface (PES) affect the nuclear motion, are involved in many photophysical and photochemical phenomena, such as vision, chemi- and bioluminescence, DNA photostability, photosynthesis, etc. The theoretical study of such processes has been greatly developed during the past couple of decades and involves mostly the use of quantum or mixed quantum-classical molecular dynamics simulations. , …”

Section: Introductionmentioning

confidence: 99%

Smooth Things Come in Threes: A Diabatic Surrogate Model for Conical Intersection Optimization

Galván

Lindh

2023

J. Chem. Theory Comput.

View full text Add to dashboard Cite

The optimization of conical intersection structures is complicated by the nondifferentiability of the adiabatic potential energy surfaces. In this work, we build a pseudodiabatic surrogate model, based on Gaussian process regression, formed by three smooth and differentiable surfaces that can adequately reproduce the adiabatic surfaces. Using this model with the restricted variance optimization method results in a notable decrease of the overall computational effort required to obtain minimum energy crossing points.

show abstract

“…Vertebrate phototransduction is a sensory signaling pathway providing the link between a physical stimulus (photon) and a change in membrane potential. The molecular reactions of the biochemical cascade involving the prototypical G protein-coupled receptors (GPCR) rhodopsin or cone opsin, a heterotrimeric G protein (transducin, G t ), a cGMP-specific phosphodiesterase (PDE), and a cyclic nucleotide-gated cation channel (CNG-channel) that is directly controlled by the intracellular cGMP concentration, are understood in quantitative terms ( Pugh and Lamb, 2000 ; Chen et al, 2022 ; Hofmann and Lamb, 2022 ). Similar holds true for the deactivation steps of each of the biochemical reactions in the photoexcitation process.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of cone specific G-protein signaling in avian photoreceptor cells

Yee

Görtemaker

Wellpott

et al. 2023

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Cone photoreceptor cells of night-migratory songbirds seem to process the primary steps of two different senses, vision and magnetoreception. The molecular basis of phototransduction is a prototypical G protein-coupled receptor pathway starting with the photoexcitation of rhodopsin or cone opsin thereby activating a heterotrimeric G protein named transducin. This interaction is well understood in vertebrate rod cells, but parameter describing protein–protein interactions of cone specific proteins are rare and not available for migratory birds. European robin is a model organism for studying the orientation of birds in the earth magnetic field. Recent findings showed a link between the putative magnetoreceptor cryptochrome 4a and the cone specific G-protein of European robin. In the present work, we investigated the interaction of European robin cone specific G protein and cytoplasmic regions of long wavelength opsin. We identified the second loop in opsin connecting transmembrane regions three and four as a critical binding interface. Surface plasmon resonance studies using a synthetic peptide representing the second cytoplasmic loop and purified G protein α-subunit showed a high affinity interaction with a KD value of 21 nM. Truncation of the G protein α-subunit at the C-terminus by six amino acids slightly decreased the affinity. Our results suggest that binding of the G protein to cryptochrome can compete with the interaction of G protein and non-photoexcited long wavelength opsin. Thus, the parallel presence of two different sensory pathways in bird cone photoreceptors is reasonable under dark-adapted conditions or during illumination with short wavelengths.

show abstract

Rhodopsin, light-sensor of vision

Cited by 45 publications

References 557 publications

Mammalian Fuel Cells Produce Electric Current

Mammalian Fuel Cells Produce Electric Current

Smooth Things Come in Threes: A Diabatic Surrogate Model for Conical Intersection Optimization

Kinetics of cone specific G-protein signaling in avian photoreceptor cells

Contact Info

Product

Resources

About