Rhodostomin, a Snake Venom Disintegrin, Served as a Molecular Tool to Dissect the Integrin Function

Chang, Hsin-Hou; Lo, Szecheng J.

doi:10.1080/15569540701209823

Cited by 2 publications

(2 citation statements)

References 66 publications

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“…These snakes eat small animals and hunt by striking and envenoming to immobilize and kill prey4. Their venom comprises various coagulation-disrupting proteins15, which can cause functional alterations in coagulation factors and platelets5678910111213141516171819; thus, snakebites by vipers usually lead to inflammation and hemorrhage12520212223. Antivenom injection is a major treatment for venomous snakebites, but little high-quality data is available to support its effectiveness, particularly in cases with venom-elicited coagulopathy152425.…”

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confidence: 99%

Soluble P-selectin rescues viper venom–induced mortality through anti-inflammatory properties and PSGL-1 pathway-mediated correction of hemostasis

Sun

Chang

2016

Sci Rep

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Venomous snakebites are lethal and occur frequently worldwide each year, and receiving the antivenom antibody is currently the most effective treatment. However, the specific antivenom might be unavailable in remote areas. Snakebites by Viperidae usually lead to hemorrhage and mortality if untreated. In the present study, challenges of rattlesnake (Crotalus atrox) venom markedly increased the circulating soluble P-selectin (sP-sel) level, but not P-selectin (P-sel, Selp−/−) mutants, in wild-type mice. Because sP-sel enhances coagulation through the P-selectin ligand 1 (PSGL-1, Selplg) pathway to produce tissue factor–positive microparticles, we hypothesized that increasing the plasma sP-sel level can be a self-rescue response in hosts against snake venom–mediated suppression of the coagulation system. Confirming our hypothesis, our results indicated that compared with wild-type mice, Selp−/− and Selplg−/− mice were more sensitive to rattlesnake venom. Additionally, administration of recombinant sP-sel could effectively reduce the mortality rate of mice challenged with venoms from three other Viperidae snakes. The antivenom property of sP-sel is associated with improved coagulation activity in vivo. Our data suggest that the elevation of endogenous sP-sel level is a self-protective response against venom-suppressed coagulation. The administration of recombinant sP-sel may be developed as a new strategy to treat Viperidae snakebites.

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confidence: 99%

Soluble P-selectin rescues viper venom–induced mortality through anti-inflammatory properties and PSGL-1 pathway-mediated correction of hemostasis

Sun

Chang

2016

Sci Rep

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“…CRL-1658) were maintained in Dulbecco's modified Eagle's medium (DMEM) (BioWest) containing 10% heatinactivated fetal bovine serum (Biological Industries, Beit Haemek, Israel), 2 mM l-glutamine (BioWest), 100 U/L of penicillin (BioWest), 100 mg/mL of streptomycin (BioWest), and non-essential amino acid (BioWest). Cell adhesion and inhibition assays were performed according to our previously described methods (Chang et al, 1993(Chang et al, , 1997aChang and Lo, 1998;Chang et al, 1999Chang et al, , 2001Chang et al, , 2002Chang et al, , 2003Chang et al, 2005;Sun et al, 2005;Chang and Lo, 2007). Recombinant proteins (1.2 g/30 L/cover slip) were coated on cover slips at 37 • C for 1 h in 6-well microtiter plates, and then blocked with 5% bovine serum albumin (BSA) at 37 • C for a further hour.…”

Section: Cell Culture and Cell-adhesion/inhibition Assaymentioning

confidence: 99%

Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein

Chang

Chen

Lin

et al. 2014

Journal of Virological Methods

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Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10-25 residues. Following analyses, 2 peptides spanning the 436-445 and 437-461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV.

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Rhodostomin, a Snake Venom Disintegrin, Served as a Molecular Tool to Dissect the Integrin Function

Cited by 2 publications

References 66 publications

Soluble P-selectin rescues viper venom–induced mortality through anti-inflammatory properties and PSGL-1 pathway-mediated correction of hemostasis

Soluble P-selectin rescues viper venom–induced mortality through anti-inflammatory properties and PSGL-1 pathway-mediated correction of hemostasis

Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein

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