Rhodotorula glutinis cultivation on cassava wastewater for carotenoids and fatty acids generation

Ribeiro, José Evangelista Santos; Sant’Ana, Amanda Marília da Silva; Martini, Mina; Sorce, Carlo; Andreucci, Andrea; Melo, Débora Jamila Nóbrega de; Silva, Flávio Luiz Honorato da

doi:10.1016/j.bcab.2019.101419

Cited by 41 publications

(26 citation statements)

References 38 publications

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“…Regarding species of the genus Rhodotorula , lipid concentration has been reported between 1.5 and 3 g/L. For R. glutinis , lipid production was 3 g/L [ 77 ], while using cassava wastewater, maximum production of 1.71 g/L was reached [ 78 ]. In the case of strains from R. mucilaginosa , lipid content reached a concentration of 1.76 g/L when dried durian peel was used as a substrate.…”

Section: Resultsmentioning

confidence: 99%

Conversion of banana peel into diverse valuable metabolites using an autochthonous Rhodotorula mucilaginosa strain

et al. 2022

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Low-cost substrates are an exciting alternative for bioprocesses; however, their complexity can affect microorganism metabolism with non-desirable outcomes. This work evaluated banana peel extract (BPE) as a growth medium compared to commercial Yeast-Malt (YM) broth in the native and non-conventional yeast Rhodotorula mucilaginosa UANL-001L. The production of carotenoids, fatty acids, and exopolysaccharides (EPS) was also analyzed. Biomass concentration (3.9 g/L) and growth rate (0.069 g/h) of Rhodotorula mucilaginosa UANL-001L were obtained at 200 g/L of BPE. Yields per gram of dry biomass for carotenoids (317 µg/g) and fatty acids (0.55 g/g) showed the best results in 150 g/L of BPE, while 298 µg/g and 0.46 mg/g, respectively, were obtained in the YM broth. The highest yield of EPS was observed in 50 g/L of BPE, a two-fold increase (160.1 mg/g) compared to the YM broth (76.3 mg/g). The fatty acid characterization showed that 100 g/L of BPE produced 400% more unsaturated compounds (e.g., oleic and ricinoleic acid) than the YM broth. Altogether, these results indicate that BPE is a suitable medium for producing high-value products with potential industrial applications.

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Section: Resultsmentioning

confidence: 99%

Conversion of banana peel into diverse valuable metabolites using an autochthonous Rhodotorula mucilaginosa strain

et al. 2022

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“…Similarly, spent coffee grains were also employed in a bioprocess involving the yeast Sporobolomyces roseus, and it was observed that the maximum carotenoid concentration was 12.59 mg/L, supporting the feasibility of the production of enriched yeast biomass by the utilization of this byproduct (Petrik et al, 2014). Santos-Ribeiro et al (2019) proposed the use of cassava wastewater as a sole nutrient source for the cultivation of R. glutinis. The results recorded showed elevated values of cell concentration (10.28 g/L), carotenoids (0.98 mg/L), and lipids (1.34 g/L).…”

Section: Biopigmentsmentioning

confidence: 87%

“…Some carotenigenic yeasts are also remarkable oil producers; therefore, agroindustrial byproducts can be employed as substrates for the generation of both biopigments and microbial oil. For instance, microbial oil and carotenoids were obtained simultaneously by oleaginous yeast (R. glutinis and Rhodotorula gracilis) grown on cassava wastewater (Santos-Ribeiro et al, 2019) and potato wastewater combined with raw glycerol (Kot et al, 2020). Single-cell oil is an expensive biomolecule, especially in a biorefinery context, because fatty acids and glycerol can be used as precursors of a wide range of value-added products, such as biodiesel, emulsifiers, and polymers (Jin et al, 2015).…”

Section: Biopigmentsmentioning

confidence: 99%

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

et al. 2020

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The integrated approach in biorefinery mainly involves the utilization of various agroindustrial byproducts such as raw materials for the production of several biobased products like biofuels, bioenergy, and other high-value chemicals. Biofuels are the backbone of biorefineries, however, production of value-added biomolecules such as biopigments, biopolymers, biosurfactants, and nutritional yeast has been attracting great attention. The production of these biomolecules using traditional approaches has been extensively studied in the last few years owing to their promising application in different industries such as chemical, food/feed, and pharmaceuticals for the development of novel products for mankind. Moreover, the production of such biomolecules using lignocellulosic, starchy, and some other agroindustrial byproducts is still not fully explored. Hence, there is a huge scope in the development of sustainable biorefining approaches to make the technology cost-effective. The lignocellulosic biomasses usually used in biorefineries are mainly composed of cellulose, hemicellulose, and lignin, whereas starchy materials, besides starch, usually contain, protein, lipids, and some micronutrients. The processing of these biomasses through successive steps like pretreatments, enzymatic hydrolysis, and fermentation is essentially required to obtained final biobased products. Considering certain bottlenecks of above-mentioned conventional biorefineries approaches, new technologies have been proposed for the improved pretreatment of biomass and efficient enzymatic hydrolysis in order to minimize the concentration of toxic inhibitors in resulting hydrolysate. In this review, we highlighted the different agroindustrial byproducts and their applications for the production of valuable biorefinery products.

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“…can accumulate lipids from lignocellulose-derived substrates such as xylose, glucose [35], and acetate [30]. Furthermore, the yeast strains from this genus have been found to remove low-molecular weight pollutants from lignocellulosic wastewater, and to accumulate lipids when grown on sugar supplemented lignocellulosic wastewater [10][11][12]14,36]. Furthermore, Rhodotorula kratochvilovae has been found to remove lignin and suspended solids from pulp and paper mill effluent supplemented with glucose.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts R. kratochvilovae EXF7516 and C. oleaginosum ATCC 20509

et al. 2022

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During the conversion of lignocellulose, phenolic wastewaters are generated. Therefore, researchers have investigated wastewater valorization processes in which these pollutants are converted to chemicals, i.e., lipids. However, wastewaters are lean feedstocks, so these valorization processes in research typically require the addition of large quantities of sugars and sterilization, which increase costs. This paper investigates a repeated batch fermentation strategy with Rhodotorula kratochvilovae EXF7516 and Cutaneotrichosporon oleaginosum ATCC 20509, without these requirements. The pollutant removal and its conversion to microbial oil were evaluated. Because of the presence of non-monomeric substrates, the ligninolytic enzyme activity was also investigated. The repeated batch fermentation strategy was successful, as more lipids accumulated every cycle, up to a total of 5.4 g/L (23% cell dry weight). In addition, the yeasts consumed up to 87% of monomeric substrates, i.e., sugars, aromatics, and organics acids, and up to 23% of non-monomeric substrates, i.e., partially degraded xylan, lignin, cellulose. Interestingly, lipid production was only observed during the harvest phase of each cycle, as the cells experienced stress, possibly due to oxygen limitation. This work presents the first results on the feasibility of valorizing non-sterilized lignocellulosic wastewater with R. kratochvilovae and C. oleaginosum using a cost-effective repeated batch strategy.

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Rhodotorula glutinis cultivation on cassava wastewater for carotenoids and fatty acids generation

Cited by 41 publications

References 38 publications

Conversion of banana peel into diverse valuable metabolites using an autochthonous Rhodotorula mucilaginosa strain

Conversion of banana peel into diverse valuable metabolites using an autochthonous Rhodotorula mucilaginosa strain

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts R. kratochvilovae EXF7516 and C. oleaginosum ATCC 20509

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