Rhythmic contraction and intracellular Ca<sup>2 + </sup>oscillatory rhythm in spontaneously beating cultured cardiac myocytes

Nakayama, Yukako; Kikuchi, Kôichi; Yoneyama, Mitsuru; Hachiro, Takeru

doi:10.1080/09291010500124597

Cited by 7 publications

(4 citation statements)

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“…This concentration of BDM almost completely terminates the spontaneous contractile activity of cultured cardiac myocytes (Nakayama et al, 2005). The treatment did not significantly change the chemiluminescence signals (see Figure 2d and 2e), suggesting that the measured changes in the chemiluminescence reflected the concentration of extracellular ATP, not artifacts caused by the cyclic movements of the extracellular fluid.…”

Section: Resultsmentioning

confidence: 84%

“…The intracellular concentration of free Ca 2þ also changes rhythmically in association with the rhythmic contraction of myocytes (Ca 2þ oscillation). Such a Ca 2þ oscillation was also synchronized among cultured cardiac myocytes (Nakayama et al, 2005). It is generally believed that gap junctional intercellular communication plays an important role in the intercellular synchronization of intracellular Ca 2þ oscillation (Kimura et al, 1995).…”

Section: Introductionmentioning

confidence: 88%

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Fluctuations in the Concentration of Extracellular ATP Synchronized with Intracellular Ca²⁺Oscillatory Rhythm in Cultured Cardiac Myocytes

Kikuchi

Nakayama

2007

Chronobiology International

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Isolated and cultured neonatal cardiac myocytes contract spontaneously and cyclically. The intracellular concentration of free Ca(2+) also changes rhythmically in association with the rhythmic contraction of myocytes (Ca(2+) oscillation). Both the contraction and Ca(2+) oscillatory rhythms are synchronized among myocytes, and intercellular communication via gap junctions has been considered primarily responsible for the synchronization. However, a recent study has demonstrated that intercellular communication via extracellular ATP-purinoceptor signaling is also involved in the intercellular synchronization of intracellular Ca(2+) oscillation. In this study, we aim to elucidate whether the concentration of extracellular ATP changes cyclically and contributes to the intercellular synchronization of Ca(2+) oscillation among myocytes. In almost all the cultured cardiac myocytes at four days in vitro (4 DIV), intracellular Ca(2+) oscillations were synchronized with each other. The simultaneous measurement of the concentration of extracellular ATP and intracellular Ca(2+) revealed the extracellular concentration of ATP actually oscillated concurrently with the intracellular Ca(2+) oscillation. In addition, power spectrum and cross-correlation analyses suggested that the treatment of cultured cardiac myocytes with suramin, a blocker of P2 purinoceptors, resulted in the asynchronization of Ca(2+) oscillatory rhythms among cardiac myocytes. Treatment with suramin also resulted in a significant decrease in the amplitudes of the cyclic changes in both intracellular Ca(2+) and extracellular ATP. Taken together, the present study demonstrated the possibility that the concentration of extracellular ATP changes cyclically in association with intracellular Ca(2+), contributing to the intercellular synchronization of Ca(2+) oscillation among cultured cardiac myocytes.

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Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 88%

Fluctuations in the Concentration of Extracellular ATP Synchronized with Intracellular Ca²⁺Oscillatory Rhythm in Cultured Cardiac Myocytes

Kikuchi

Nakayama

2007

Chronobiology International

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“…The intracellular concentration of free Ca 2+ also changed cyclically in association with the spontaneous rhythmic contraction of cardiac myocytes (Nakayama et al, 2005b). We first investigated whether the spontaneous cyclic changes in the concentration of free Ca…”

Section: Resultsmentioning

confidence: 99%

“…The intracellular concentration of free Ca 2+ also changes rhythmically in association with the rhythmic contraction of myocytes (Ca 2+ oscillation). Such a Ca 2+ oscillation was also synchronized among cultured cardiac myocytes (Nakayama et al, 2005b). It is generally believed that gap junctional intercellular communication plays an important role in the intercellular synchronization of intracellular Ca 2+ oscillation (Kimura et al, 1995).…”

Section: Introductionmentioning

confidence: 85%

Possible involvement of ATP-purinoceptor signalling in the intercellular synchronization of intracellular Ca2+ oscillation in cultured cardiac myocytes

et al. 2007

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SummaryIsolated and cultured neonatal cardiac myocytes contract spontaneously and cyclically.The contraction rhythms of two isolated cardiac myocytes, each of which beats at different frequencies at first, become synchronized after the establishment of mutual contacts, suggesting that mutual entrainment occurs due to electrical and/or mechanical interactions between two myocytes. The intracellular concentration of free Ca 2+ also changes rhythmically in association with the rhythmic contraction of myocytes (Ca

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Rhythmic Fluctuations in Intracellular Mg2+ in Spontaneously Beating Cultured Cardiac Myocytes

et al. 2008

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Rhythmic contraction and intracellular Ca^2 +oscillatory rhythm in spontaneously beating cultured cardiac myocytes

Cited by 7 publications

References 17 publications

Fluctuations in the Concentration of Extracellular ATP Synchronized with Intracellular Ca²⁺Oscillatory Rhythm in Cultured Cardiac Myocytes

Fluctuations in the Concentration of Extracellular ATP Synchronized with Intracellular Ca²⁺Oscillatory Rhythm in Cultured Cardiac Myocytes

Possible involvement of ATP-purinoceptor signalling in the intercellular synchronization of intracellular Ca2+ oscillation in cultured cardiac myocytes

Rhythmic Fluctuations in Intracellular Mg2+ in Spontaneously Beating Cultured Cardiac Myocytes

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Rhythmic contraction and intracellular Ca2 + oscillatory rhythm in spontaneously beating cultured cardiac myocytes

Cited by 7 publications

References 17 publications

Fluctuations in the Concentration of Extracellular ATP Synchronized with Intracellular Ca2+Oscillatory Rhythm in Cultured Cardiac Myocytes

Fluctuations in the Concentration of Extracellular ATP Synchronized with Intracellular Ca2+Oscillatory Rhythm in Cultured Cardiac Myocytes

Possible involvement of ATP-purinoceptor signalling in the intercellular synchronization of intracellular Ca2+ oscillation in cultured cardiac myocytes

Rhythmic Fluctuations in Intracellular Mg2+ in Spontaneously Beating Cultured Cardiac Myocytes

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Rhythmic contraction and intracellular Ca^2 +oscillatory rhythm in spontaneously beating cultured cardiac myocytes

Fluctuations in the Concentration of Extracellular ATP Synchronized with Intracellular Ca²⁺Oscillatory Rhythm in Cultured Cardiac Myocytes

Fluctuations in the Concentration of Extracellular ATP Synchronized with Intracellular Ca²⁺Oscillatory Rhythm in Cultured Cardiac Myocytes