Rhythmic Food Intake Drives Rhythmic Gene Expression More Potently than the Hepatic Circadian Clock in Mice

Greenwell, Ben J.; Trott, Alexandra J.; Beytebiere, Joshua R.; Pao, Shanny; Bosley, Alexander; Beach, Erin; Finegan, Patrick; Hernandez, Christopher; Menet, Jérôme S.

doi:10.2139/ssrn.3325032

Cited by 33 publications

(55 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The imaging sessions of sleeping mice started at 9-10 a.m. (ZT 1-2), the beginning of light phase, and lasted until 3-6 p.m. (ZT 7-10). The mice did not have access to food or water while sleeping under the microscope, however, in natural conditions mice feed almost exclusively during the dark phase, ZT 12-24 [59][60][61] . First signs of drowsiness, such as high delta power in the ECoG signal and eyes closing, were observed 15-45 min after head-fixation, and typically mice spent 90-120 min head-fixed under the microscope before falling asleep.…”

Section: Methodsmentioning

confidence: 99%

Astrocytic Ca2+ signaling is reduced during sleep and is involved in the regulation of slow wave sleep

et al. 2020

View full text Add to dashboard Cite

Astrocytic Ca 2+ signaling has been intensively studied in health and disease but has not been quantified during natural sleep. Here, we employ an activity-based algorithm to assess astrocytic Ca 2+ signals in the neocortex of awake and naturally sleeping mice while monitoring neuronal Ca 2+ activity, brain rhythms and behavior. We show that astrocytic Ca 2+ signals exhibit distinct features across the sleep-wake cycle and are reduced during sleep compared to wakefulness. Moreover, an increase in astrocytic Ca 2+ signaling precedes transitions from slow wave sleep to wakefulness, with a peak upon awakening exceeding the levels during whisking and locomotion. Finally, genetic ablation of an important astrocytic Ca 2+ signaling pathway impairs slow wave sleep and results in an increased number of microarousals, abnormal brain rhythms, and an increased frequency of slow wave sleep state transitions and sleep spindles. Our findings demonstrate an essential role for astrocytic Ca 2+ signaling in regulating slow wave sleep.

show abstract

Section: Methodsmentioning

confidence: 99%

Astrocytic Ca2+ signaling is reduced during sleep and is involved in the regulation of slow wave sleep

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Another study fed mice with high‐fat diet (HFD) during active phase (nighttime) or sleep phase (daytime) and found that mice with sleep phase feeding had increased body weights as well as hepatic concentrations of free fatty acids (FFAs) and triglycerides compared to mice with active phase feeding . Arrhythmic feeding of mice with one‐eighth portion of daily food intake given every 3 hours disrupted rhythmic expression of over 70% of transcriptomes in the liver . Circadian disruption induces leptin resistance in mice, which may lead to obesity or metabolic syndromes, indicating different impacts of food intake in the liver depending on the timing of intake .…”

Section: Functional Roles and Therapeutic Potentials Of Melatonin Andmentioning

confidence: 99%

“…34 Arrhythmic feeding of mice with one-eighth portion of daily food intake given every 3 hours disrupted rhythmic expression of over 70% of transcriptomes in the liver. 35 Circadian disruption induces leptin resistance in mice, which may lead to obesity or metabolic syndromes, indicating different impacts of food intake in the liver depending on the timing of intake. 36 A previous study has demonstrated that Alb-Cre-mediated liver-specific BMAL1 knockout mice have elevated mRNA methylation especially in peroxisome proliferator-activated receptor alpha (PPARα) in the liver.…”

Section: Nonalcoholic Fatty Liver Diseasementioning

confidence: 99%

Melatonin and circadian rhythms in liver diseases: Functional roles and potential therapies

Sato

Meng

Francis

et al. 2020

Journal of Pineal Research

View full text Add to dashboard Cite

Circadian rhythms and clock gene expressions are regulated by the suprachiasmatic nucleus in the hypothalamus, and melatonin is produced in the pineal gland. Although the brain detects the light through retinas and regulates rhythms and melatonin secretion throughout the body, the liver has independent circadian rhythms and expressions as well as melatonin production. Previous studies indicate the association between circadian rhythms with various liver diseases, and disruption of rhythms or clock gene expression may promote liver steatosis, inflammation, or cancer development. It is well known that melatonin has strong antioxidant effects. Alcohol drinking or excess fatty acid accumulation produces reactive oxygen species and oxidative stress in the liver leading to liver injuries. Melatonin administration protects these oxidative stress‐induced liver damage and improves liver conditions. Recent studies have demonstrated that melatonin administration is not limited to antioxidant effects and it has various other effects contributing to the management of liver conditions. Accumulating evidence suggests that restoring circadian rhythms or expressions as well as melatonin supplementation may be promising therapeutic strategies for liver diseases. This review summarizes recent findings for the functional roles and therapeutic potentials of circadian rhythms and melatonin in liver diseases.

show abstract

“…In one study, food intake was provided ad libitum (as much as desired), as arrhythmic (every 3 h) or night‐restricted (only at night). Strikingly, over 70% of rhythmically transcribed genes were oscillatory due to the periodicity of nutrient availability rather than the molecular clock, and the majority of these genes are related to metabolic and nutrient response pathways (Greenwell et al , ). In addition, another group found that autophagy oscillates in multiple tissues to regulate protein degradation.…”

Section: Tfeb/tfe3 Connect the Nutrient Sensitive Clock To The Circadmentioning

confidence: 99%

Autophagy: clocking in for the night shift

Brooks

Dang

2019

The EMBO Journal

View full text Add to dashboard Cite

Daily rhythms of biological activity, such as cycles of sleep–wake and feeding–fasting, are coupled to cell‐autonomous circadian clocks to synchronize organismal food intake with cellular bioenergetics. Food intake during wake stimulates biosynthesis followed by rest periods of autophagy, which degrades damaged macromolecules and recycles them as nutrients to enhance fitness. In this issue of The EMBO Journal, Pastore et al discovered that regulators of autophagy, TFEB and TFE3, are activated during the rest phase and ultimately drive the expression of Rev‐erbα, a component of the core circadian molecular clock, coupling autophagy and the clock.

show abstract

Rhythmic Food Intake Drives Rhythmic Gene Expression More Potently than the Hepatic Circadian Clock in Mice

Cited by 33 publications

References 56 publications

Astrocytic Ca2+ signaling is reduced during sleep and is involved in the regulation of slow wave sleep

Astrocytic Ca2+ signaling is reduced during sleep and is involved in the regulation of slow wave sleep

Melatonin and circadian rhythms in liver diseases: Functional roles and potential therapies

Autophagy: clocking in for the night shift

Contact Info

Product

Resources

About