Riboflavin detection by α-Fe₂O₃/MWCNT/AuNPs-based composite and a study of the interaction of riboflavin with DNA

Abstract

The electrochemical behavior of riboflavin (RF) at a glassy carbon electrode modified with α-Fe2O3/MWCNT/AuNPs was investigated by cyclic voltammetry (CV) and square wave voltammetry (SWV).

Label-Free and Ultrasensitive Electrochemical DNA Biosensor Based on Urchinlike Carbon Nanotube-Gold Nanoparticle Nanoclusters

“…The detection limit was estimated to be 5.2 fM, according to the 3σ/a criterion (where a is the slope of the liner regression, and σ is the standard deviation of the blank analysis), which is much lower than those reported in the literature (AuNP-decorated CNTs-assisted DNA sensing) (Table ). − A comparison experiment was also performed (Figure S4), which demonstrated the detection limit of the proposed biosensor increased 25 times with and without CNT involvement (Figure S5). This high sensitivity would most likely be related to the structural feature of the 3D radial CNT-AuNP nanocomposites.…”

Mechanistic Insights into the Voltammetric Determination of Riboflavin at Poly (Serine) Modified Graphite and Carbon Nanotube Composite Paste Electrode

“…In the body, RF is mostly found to be the basic factor of two coenzymes that is, Flavin Adenine Dinucleotide (FAD) and Flavin Mono Nucleotide (FMN). [1,2] They have a predominant role for the mitochondrial energy production carried out by electron transport chain, oxidation of fatty acids, metabolism of niacin, folate and pyridoxine, catabolism of dopamine and serotonin, activation of glutathione reductase and thioredoxin reductase. [3][4][5] Therefore, its need is important for human health as it is not synthesized within the body, so RF is ingested by ingestion of food like meat, fatty fish, egg, particular fruits and vegetables mainly dark green vegetables.…”

DNA Damage and Apoptosis Induction in Cancer Cells by Chemically Engineered Thiolated Riboflavin Gold Nanoassembly

“…The therapeutic applications of nanoparticles in regulating live cell functions have become the most challenging aspects of nanobiotechnology , and nanomedicine. , The gold nanoparticles ( AuNPs ) have received considerable attention in the biological and biomedical communities due to their potential use in diagnostic and therapeutic applications. ,− The AuNPs are usually conjugated with drugs to enhance the efficiency of targeted delivery, thus holding great promise in tumor therapy and drug delivery due to their ability to serve as biocompatible scaffolds for intracellular targeting. , Moreover, their nanoscale size allows them to enter cells and to target the different organelles, i.e., endosome, nucleus, and mitochondria. − The riboflavin ( Rf ) is widely distributed in human tissues in free or conjugated forms and plays an important role in formation of flavoprotein enzyme. , The Rf that consists of an isoalloxazin moiety and a ribityl chain possesses mixed features of hydrophobic and hydrophilic functionalities, , which generates its affinity toward different biological macromolecules including proteins and DNA . Moreover, the Rf receptor protein constitutes one type of tumor biomarkers due to their overexpression in malignant cells of human breast and prostate cancers. , …”

“…14,15 The Rf that consists of an isoalloxazin moiety and a ribityl chain possesses mixed features of hydrophobic and hydrophilic functionalities, 16,17 which generates its affinity toward different biological macromolecules including proteins and DNA. 18 Moreover, the Rf receptor protein constitutes one type of tumor biomarkers due to their overexpression in malignant cells of human breast and prostate cancers. 19,20 Taking advantage of the above properties of Rf and AuNPs, we have attempted to design and synthesize one of their modified conjugates, RfS@AuNPs, which could facilitate significant photophysical changes due to DNA intercalation in vitro.…”