Ribonuclease activity of Petunia inflata S proteins is essential for rejection of self-pollen.

Huang, Shihshieh; Lee, Hyun Sook; Karunanandaa, Balasulojini; Kao, Teh Hui

doi:10.1105/tpc.6.7.1021

Cited by 242 publications

(74 citation statements)

References 26 publications

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“…That S C2 -haplotype is defective in the pollen function of S 17 -haplotype was further confirmed by the characterization of S C2 -RNase and S 17 -RNase and subsequent cloning and sequencing of their cDNAs. The sequences of these two S-RNases are completely identical, and both have normal RNase activity, which is required for rejection of self-pollen during the SI response (Huang et al, 1994).…”

Section: Discussionmentioning

confidence: 92%

Breakdown of Self-Incompatibility in a Natural Population ofPetunia axillaris Caused by Loss of Pollen Function

et al. 2003

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Although Petunia axillaris subsp. axillaris is described as a self-incompatible taxon, some of the natural populations we have identified in Uruguay are composed of both self-incompatible and self-compatible plants. Here, we studied the selfincompatibility (SI) behavior of 50 plants derived from such a mixed population, designated U83, and examined the cause of the breakdown of SI. Thirteen plants were found to be self-incompatible, and the other 37 were found to be selfcompatible. A total of 14 S-haplotypes were represented in these 50 plants, including two that we had previously identified from another mixed population, designated U1. All the 37 self-compatible plants carried either an S C1 -or an S C2 -haplotype. S C1 S C1 and S C2 S C2 homozygotes were generated by self-pollination of two of the self-compatible plants, and they were reciprocally crossed with 40 self-incompatible S-homozygotes (S 1 S 1 through S 40 S 40 ) generated from plants identified from three mixed populations, including U83. The S C1 S C1 homozygote was reciprocally compatible with all the genotypes examined. The S C2 S C2 homozygote accepted pollen from all but the S 17 S 17 homozygote (identified from the U1 population), but the S 17 S 17 homozygote accepted pollen from the S C2 S C2 homozygote. cDNAs encoding S C2 -and S 17 -RNases were cloned and sequenced, and their nucleotide sequences were completely identical. Analysis of bud-selfed progeny of heterozygotes carrying S C1 or S C2 showed that the SI behavior of S C1 and S C2 was identical to that of S C1 and S C2 homozygotes, respectively. All these results taken together suggested that the S C2 -haplotype was a mutant form of the S 17 -haplotype, with the defect lying in the pollen function. The possible nature of the mutation is discussed.Naturally occurring or induced (e.g. by radiation) self-compatible mutants of self-incompatible species in the Solanaceae have provided useful materials for studying the mechanism of the S-RNase-mediated self-incompatibility (SI) system. For example, molecular genetic studies of a self-compatible line of Lycopersicon peruvianum have suggested that the RNase activity of S-RNases is an integral part of the SI mechanism because mutations in the S-RNase gene rendering its protein product without the RNase activity cause the breakdown of SI (Kowyama et al., 1994a;Royo et al., 1994). Moreover, cytological and molecular genetic studies of x-ray irradiationinduced self-compatible mutants have uncovered a phenomenon, called competitive interaction, where duplication of the S-locus region renders pollen grains carrying two different pollen S-alleles unable to function in SI (de Nettancourt, 1977;Golz et al., 2000). Studies of self-compatible mutants have also led to the identification of loci unlinked to the S-locus that are required for the function of the S-RNase gene or the yet unidentified pollen S-gene. For example, mutations in the HT gene, which encodes a small Asn-rich protein (McClure et al., 1999), are partially responsible for the breakdown o...

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Section: Discussionmentioning

confidence: 92%

Breakdown of Self-Incompatibility in a Natural Population ofPetunia axillaris Caused by Loss of Pollen Function

et al. 2003

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“…En Solanaceae el locus S codifica para una glicoproteína del estilo (SLG: Style Locus Glycoprotein), la cual es secretada al espacio extracelular del mismo (Cheung et al, 2000). Se propone que estas proteínas actúan como agente citotóxico S-específico degradando los tubos polínicos incompatibles (Huang et al, 1994). Teniendo en cuenta que el modelo de auto-incompatibilidad gametofítica propone que la incompatibilidad se expresa a nivel del estilo (Pandey, 1969;Nettancourt, 2005), se podría asumir que procesos semejantes pudieran regir la incompatibilidad entre diferentes géneros, como en los casos presentados en este trabajo (Liedl y Anderson, 1994).…”

Section: Introductionunclassified

MEJORAMIENTO DE PLANTAS NATIVAS SUDAMERICANAS: ALTAS TEMPERATURAS COMO HERRAMIENTA PARA SUPERAR BARRERAS PRECIGÓTICAS EN EL COMPLEJO Calibrachoa-Nierembergia

Milicia

Coviella

Soto

et al. 2016

Chil. j. agric. anim. sci.

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RESUMENEl objetivo del presente trabajo fue evaluar la utilización de altas temperaturas como metodología para superar las barreras de incompatibilidad precigótica en cruzamientos interespecíficos entre especies del género Calibrachoa y Nierembergia. Se realizaron los cruzamientos en las combinaciones N. scoparia x C. ovalifolia, N. scopaira x C. caesia, N. linariaefolia x C. thymifolia y N. linariaefolia x C. ovalifolia, utilizando diferentes genotipos. Las plantas con los botones florales preparados para polinizar fueron colocadas en estufa a 50ºC durante 5 minutos para N. scoparia y 2,5 minutos para N. linariaefolia. Cumplido el tiempo se retiraron de la estufa y se polinizaron con polen fresco de Calibrachoa spp. Se evaluó el porcentaje de cruzamientos con llegada de tubos polínicos al estilo y al ovario. La aplicación de altas temperaturas a gineceos de N. scoparia se presentó como una metodología exitosa en la combinación N. scoparia x C. caesia, debido a que en todos los casos la cantidad de tubos polínicos en ovario fue mayor al testigo. En el caso de N. linariaefolia los resultados muestran que si bien hay una mejora con este método, no hubo un incremento de tubos polínicos en ovarios. Los resultados de este trabajo permitieron generar nuevos conocimientos sobre las relaciones intergenéricas, las barreras de incompatibilidad y aspectos vinculados con la biología floral, que sentarán las bases para la obtención de híbridos.Palabras clave: Solanaceae, hibridación intergenérica, tubo polínico, incompatibilidad ABSTRACTThe aim of this study was to evaluate the use of high temperatures to overcome prezygotic interspecific incompatibility barriers in crosses between species of Calibrachoa and Nierembergia. The following combinations were evaluated: N. scoparia x C. ovalifolia, N. scoparia x C. caesia, N. linariaefolia x C. thymifolia and N. linariaefolia x C. ovalifolia, using different genotypes. The plants with prepared buds were placed in a stove at 50°C for 5 minutes for N. scoparia and 2.5 minutes for N. linariaefolia. Subsequently, the plants are removed from the oven and pollinated with fresh pollen. The percentage of crosses of pollen tubes to the style and ovary was evaluated. Application

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“…Haploid pollen carrying an S-haplotype identical to one of two S-haplotypes carried by the diploid pistil is recognized as self-pollen and is rejected. The S-RNase gene controls female specificity at the S-locus, and RNase activity is essential for the SI response (Huang et al, 1994). It is generally accepted that SRNases act to degrade RNA of self-pollen tubes resulting in the inhibition of fertilization, but many details of the mechanism are unknown.…”

Section: F-box Proteins and Self-incompatibilitymentioning

confidence: 99%

F-Box Proteins Take Center Stage

Eckardt¹

2004

Plant Cell

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Ribonuclease activity of Petunia inflata S proteins is essential for rejection of self-pollen.

Cited by 242 publications

References 26 publications

Breakdown of Self-Incompatibility in a Natural Population ofPetunia axillaris Caused by Loss of Pollen Function

Breakdown of Self-Incompatibility in a Natural Population ofPetunia axillaris Caused by Loss of Pollen Function

MEJORAMIENTO DE PLANTAS NATIVAS SUDAMERICANAS: ALTAS TEMPERATURAS COMO HERRAMIENTA PARA SUPERAR BARRERAS PRECIGÓTICAS EN EL COMPLEJO Calibrachoa-Nierembergia

F-Box Proteins Take Center Stage

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