Ribonucleotide and ribonucleoside determination by ambient pressure ion mobility spectrometry

Abstract

Reduced mobilities, resolving powers and detection limits for 12 ribonucleotides and 4 ribonucleosides were measured by ambient pressure electrospray ionization ion mobility spectrometry (ESI-IMS). With the instrument used in this study it was possible to separate some of these compounds within the mixtures. In addition, the detection limits reported for the ribonucleotides and ribonucleosides ranged from 15 to 300 picomoles whereas resolving power ranged from 41 to 56 suggesting that ambient pressure ESI-IMS may be used for their rapid and sensitive separation and detection. This short report demonstrates that it was possible to use IMS for the separation of nucleotides and nucleosides in less than one second. The application holds great promise for nucleotide analysis in the area of separating DNA fragments in genome sequencing and also for forensics DNA typing examinations used for the identification of blood stains in crime scenes and paternity testing.

Analysis of Psychoactive Cathinones and Tryptamines by Electrospray Ionization Atmospheric Pressure Ion Mobility Time-of-Flight Mass Spectrometry

“…The analyte detection limit (dl) was determined from the spectra. 56,57 By assuming a linear response, the minimum concentration closer to the detection limit whose signal was greater than the instrument noise was electrosprayed into the instrument. The detection limits at 3σ were estimated using statistics as follows:…”

“…The analyte detection limit (dl) was determined from the spectra. , By assuming a linear response, the minimum concentration closer to the detection limit whose signal was greater than the instrument noise was electrosprayed into the instrument. The detection limits at 3σ were estimated using statistics as follows: analyte dl = t α , n RSD x false( amount electrosprayed false) 100 % where t α, n is the t -table value for n – 1 degrees of freedom, α is the confidence interval, n is the number of replicate measurements minus 1, and RSD x is the relative standard deviation…”

Computational fluid dynamics-Monte Carlo method for calculation of the ion trajectories and applications in ion mobility spectrometry

“…IMS is a well-known method to analyze chemical substance using gas-phase mobility in a weak electric field [14][15][16], and is more and more used in the detection of explosives, drugs, chemical warfare agents, biological and medical samples [17][18][19][20][21][22][23][24][25]. Unlike the mass spectrometry [26,27], which operates in vacuum, IMS detects chemical compounds at atmospheric pressure.…”

Novel ion drift tube for high-performance ion mobility spectrometers based on a composite material

“…Ion mobility spectrometers (IMS) are compact analytical instruments, mainly for the rapid detection of volatile and semivolatile substances at trace levels in the range of ppt V (partsper-trillion by volume) within a few seconds [1,2]. Equipped with electrospray ionization, IMS are also used for measuring larger molecules in liquids [3][4][5]. Drift tube IMS separate ions in the gas phase under the influence of a homogeneous electric field.…”