Ribonucleotide reductase: Implications of thiol S-nitrosylation and tyrosine nitration for different subunits

Chakraborty, Surupa; Mukherjee, Prerona; Sengupta, Rajib

doi:10.1016/j.niox.2022.07.002

Cited by 4 publications

(3 citation statements)

References 163 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In light of the best additive being DTBP, we also evaluated two other types of solvents (DMF and 1,4-dioxane), unfortunately, these two solvents led to slight erosion in yield (entries [11][12]. Subsequently, the temperature effects were studied, the yields of 46% and 50% were obtained when the reactions were carried out at 50 and 90 C (Table 1, entries [13][14]. In order to improve the yields, changing the amount of DTBP from 0.4 equiv.…”

Section: Resultsmentioning

confidence: 99%

“…On account of the great importance for this kind of compounds, there are four major methods developed for synthesis of nitro‐containing compounds, such as the traditional Friedel‐Crafts nitration of aryl compounds with Conc. H 2 SO 4 /HNO 3 [11], oxidation of amino groups [12], nitration reactions of enynes with nitro source through radical pathway [13], as well as enzyme catalyzed reactions [14]. In 2017, Sun and co‐workers reported the nitration reaction of imides with NO 2 gas for the synthesis of nitro‐containing isoquinoline‐1,3‐diones (Scheme 1) [15].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis of nitro‐containing isoquinoline‐1,3‐diones with tert‐butyl nitrite

Ma,

Yu,

You

et al. 2023

Journal of Heterocyclic Chem

View full text Add to dashboard Cite

An efficient synthetic method for the nitro‐containing isoquinoline‐1,3‐diones has been achieved with tert‐butyl nitrite. This reaction features metal‐free, safety, simple operation and mild reaction conditions, which represents the first example of using stable and safe tert‐butyl nitrite as the nitro precursor for the synthesis of nitro‐containing isoquinoline‐1,3‐diones. Furthermore, both the control experiments and density functional theory calculations (DFT) have confirmed that the reaction was occurred through the radical reactions. In addition, the representative compounds 3a and 3i displayed potential good inhibitory activities for the plant height of rape and the compound 3a displayed potential promotion activity of the root length for barnyard grass.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis of nitro‐containing isoquinoline‐1,3‐diones with tert‐butyl nitrite

Ma,

Yu,

You

et al. 2023

Journal of Heterocyclic Chem

View full text Add to dashboard Cite

show abstract

“…RNR is a multi-subunit enzyme responsible for catalyzing the rate-limiting step in the synthesis of DNA ( Chakraborty et al, 2022 ). We found that iron deficiency suppressed the activity of RNR in testis.…”

Section: Discussionmentioning

confidence: 99%

Sevoflurane inhibited reproductive function in male mice by reducing oxidative phosphorylation through inducing iron deficiency

Zhang

Zuo

Zhang

et al. 2023

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Sevoflurane (Sev) is one of the commonly used inhalation anesthetic chemicals in clinics. It has great impact on spermatogenesis and fertilization in male animals. The underlying mechanism remains largely unexplored. Based on our previous research, we hypothesized that Sev induced iron metabolism disturbance in the testis and epididymis and inhibited the spermatogenesis. In this study, two-month-old C57BL/6 male mice were treated with 3% Sev for 6 h, and their fertility (including sperm concentration, sperm mobility, and the number of offspring) was evaluated. Mice testis, epididymis, and sperm were harvested and subjected to Western blot analysis and immunofluorescence analysis. Iron levels were reflected by the gene expression of iron metabolism-related proteins (including ferritin, TfR1, and FpN1) and ICP-MS and Perl’s iron staining. Electron transport and oxidative phosphorylation levels were measured by Oxygraph-2k and ATP contents. The activity of ribonucleotide reductase was evaluated by assay kit. DNA synthesis status in testis and/or epididymis was marked with BrdU. Cell proliferation was evaluated by double immunofluorescence staining of specific protein marker expression. Our results revealed that the mice exposed to Sev showed damaged testicular and epididymis structure and significantly reduced the sperm concentration, sperm motility, and fertility. Sev decreases the iron levels through down-regulating the expression of H-ferritin, L-ferritin, and FpN1, and up-regulating the expression of TfR1 in the testis and epididymis. Iron levels also significantly reduced in germ cells which decrease the number of germ cells, including sperm, Sertoli cells, and primary spermatocyte. Iron deficiency not only decreases electron transport, oxidative phosphorylation level, and ATP production but also suppresses the activity of ribonucleotide reductase and the expression of Ki67, DDX4, GATA1, and SCP3, indicating that Sev affects the spermatogenesis and development. Meanwhile, Sev impaired the blood-testis barrier by decreasing the ZO1 expression in the testis and epididymis. The damage effect induced by Sev can be significantly ameliorated by iron supplementation. In conclusion, our study illustrates a new mechanism by which Sev inhibits spermatogenesis and fertility through an oxidative phosphorylation pathway due to iron deficiency of epididymis and testis or sperm. Furthermore, the damaging effects could be ameliorated by iron supplementation.

show abstract