Ribose operon repressor (RbsR) contributes to the adhesion of Aeromonas hydrophila to Anguilla japonica mucus

Abstract

The characterization of adhesion between pathogenic bacteria and the host is critical. Pathogenic Aeromonas hydrophila was shown to adhere in vitro to the mucus of Anguilla japonica. To further investigate the adhesion mechanisms of A. hydrophila, a mini‐Tn10 transposon mutagenesis system was used to generate an insertion mutant library by cell conjugation. Seven mutants that were impaired in adhesion to mucus were selected out of 332 individual colonies, and mutant M196 was the most severely impaired strain. National Center for Biotechnology Information (NCBI) blast analysis showed that mutant M196 was inserted by mini‐Tn10 with an ORF of approximately 1,005 bp (GenBank accession numbers KP280172). This ORF is predicted to encode a protein consist of 334 amino acid, which displays the highest identity (98%) with the RbsR of A. hydrophila ATCC 7966. Random inactivation of rbsR gene affected the pleiotropic phenotypes of A. hydrophila. The adhesion ability and the survival level of the rbsR gene mutant (M196) were attenuated compared with the wild‐type and rbsR complementary type. The findings of this study indicated that RbsR plays roles in the bacterial adhesion and intracellular survival of A. hydrophila.

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

“…Aeromonas hydrophila belongs to the Aeromonadaceae family (Lin et al, ) that is widely distributed in aquatic environment (Daskalov, ; Li et al, ; Xiong et al, ). It is regarded as one of the important pathogens in freshwater cultured animals (Abdolnabi, Ina‐Salwany, Daud, Mariana, & Abdelhadi, ; Baumgartner, Ford, & Hanson, ; Zhang et al, ) and has caused serious economic losses to the global aquaculture industry (Fang, Ge, & Sin, ; Hu et al, ; Ni, Wang, Liu, & Lu, ; Pridgeon et al, ; Zhang, Yan, et al, ).…”

The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses

“…Adhesion of the pathogen to the host surface constitutes a critical step in infection initiation (Chen et al., ; Shoemaker, LaFrentz, Peatman, & Beck, ), and multiple pathogenic bacteria show strong adhesion abilities (Lin et al., ; Papadopoulou, Dalsgaard, Lindén, & Wiklund, ; Qin, Lin, Chen, Xu, & Yan, ). Bacterial adhesion is regulated by different genes (Guo et al., ; Huang, Qin, et al., ; Jiang et al., ; Xu, Huang, Su, & Yan, ).…”

secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus

“…Host mucus is abundantly found on the surface of the skin, gills, and gut lining; therefore, it is the first site of interaction between the pathogen and its host (Yan, Zhao, Wang, Zou, & Chen, 2010). Several genes have been demonstrated to be involved into the bacterial adhesion to fish mucus (Qin, Yan, Su, Li, & Zou, 2013;Qin et al, 2014;Qin, Lin, Chen, Xu, & Yan, 2016;Huang, Hu, et al, 2016;Huang et al, 2017;Lin et al, 2017). Previous researches on other bacteria have also shown that some secretion systems are necessary for adhesion, mainly through regulating the secretion of adhesin.…”