Ribosomal protein L7a binds RNA through two distinct RNA-binding domains

Russo, Giulia; Cuccurese, Monica; Monti, Gianluca; Russo, Annapina; Amoresano, Angela; Pucci, Piero; Pietropaolo, C

doi:10.1042/bj20040371

Cited by 31 publications

(31 citation statements)

References 31 publications

(61 reference statements)

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“…26 As shown in Figure 1C, the enforced expression of rpL3, in contrast to rpL7a, is associated to the upregulation of p21 gene promoter transactivation in a dose-dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Human rpL3 induces G₁/S arrest or apoptosis by modulating p21^waf1/cip1 levels in a p53-independent manner

Russo

Esposito²,

Catillo³

et al. 2012

Cell Cycle

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104

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“…26 As shown in Figure 1C, the enforced expression of rpL3, in contrast to rpL7a, is associated to the upregulation of p21 gene promoter transactivation in a dose-dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Human rpL3 induces G₁/S arrest or apoptosis by modulating p21^waf1/cip1 levels in a p53-independent manner

Russo

Esposito²,

Catillo³

et al. 2012

Cell Cycle

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104

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“…Brown II, personal communication] Currently, no detailed structural information is available for any eukaryotic L7a protein and its RNA-binding site (or motif) within the large subunit ribosomal RNA has not been defined. However, it does not appear that the human L7a protein can bind to a kink-turn motif (46). Differences in the amino acid sequence of L7a compared with Snu13p in the above-mentioned region (Figure 3B, overlined) are likely to contribute to the different RNA-binding properties displayed by the L7a protein.…”

Section: Resultsmentioning

confidence: 99%

Unusual features of fibrillarin cDNA and gene structure in Euglena gracilis: evolutionary conservation of core proteins and structural predictions for methylation-guide box C/D snoRNPs throughout the domain Eucarya

Russell

Watanabe

Charette

et al. 2005

Nucleic Acids Research

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Box C/D ribonucleoprotein (RNP) particles mediate O2′-methylation of rRNA and other cellular RNA species. In higher eukaryotic taxa, these RNPs are more complex than their archaeal counterparts, containing four core protein components (Snu13p, Nop56p, Nop58p and fibrillarin) compared with three in Archaea. This increase in complexity raises questions about the evolutionary emergence of the eukaryote-specific proteins and structural conservation in these RNPs throughout the eukaryotic domain. In protists, the primarily unicellular organisms comprising the bulk of eukaryotic diversity, the protein composition of box C/D RNPs has not yet been extensively explored. This study describes the complete gene, cDNA and protein sequences of the fibrillarin homolog from the protozoon Euglena gracilis, the first such information to be obtained for a nucleolus-localized protein in this organism. The E.gracilis fibrillarin gene contains a mixture of intron types exhibiting markedly different sizes. In contrast to most other E.gracilis mRNAs characterized to date, the fibrillarin mRNA lacks a spliced leader (SL) sequence. The predicted fibrillarin protein sequence itself is unusual in that it contains a glycine-lysine (GK)-rich domain at its N-terminus rather than the glycine-arginine-rich (GAR) domain found in most other eukaryotic fibrillarins. In an evolutionarily diverse collection of protists that includes E.gracilis, we have also identified putative homologs of the other core protein components of box C/D RNPs, thereby providing evidence that the protein composition seen in the higher eukaryotic complexes was established very early in eukaryotic cell evolution.

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“…Nuclear extracts was performed as previously reported4950, EMSA assay was performed as previously reported51.…”

Section: Methodsmentioning

confidence: 99%

rpL3 promotes the apoptosis of p53 mutated lung cancer cells by down-regulating CBS and NFκB upon 5-FU treatment

Russo

Saide

Cagliani

et al. 2016

Sci Rep

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5-FU is a chemotherapy drug commonly used for the treatment of human cancers; however drug resistance represents a major challenge for its clinical application. In the present study, we reporte that rpL3 induced by 5-FU treatment in Calu-6 cells represses CBS transcription and reduces CBS protein stability leading to a decrease of CBS protein levels. rpL3 also regulates negatively the activation of NFκB by preventing NFκB nuclear translocation through IκB-α up-regulation. Furthermore, we demonstrate that rpL3 significantly enhances the apoptosis of 5-FU treated Calu-6 cells promoting the overexpression of the pro-apoptotic proteins Bax and the inhibition of the anti-apoptotic protein Bcl-2. We finally demonstrate that rpL3 potentiates 5-FU efficacy inhibiting cell migration and invasion. Our results suggest that combination of rpL3 and 5-FU is a promising strategy for chemotherapy of lung cancers lacking functional p53 that are resistant to 5-FU.

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Ribosomal protein L7a binds RNA through two distinct RNA-binding domains

Cited by 31 publications

References 31 publications

Human rpL3 induces G₁/S arrest or apoptosis by modulating p21^waf1/cip1 levels in a p53-independent manner

Human rpL3 induces G₁/S arrest or apoptosis by modulating p21^waf1/cip1 levels in a p53-independent manner

Unusual features of fibrillarin cDNA and gene structure in Euglena gracilis: evolutionary conservation of core proteins and structural predictions for methylation-guide box C/D snoRNPs throughout the domain Eucarya

rpL3 promotes the apoptosis of p53 mutated lung cancer cells by down-regulating CBS and NFκB upon 5-FU treatment

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Ribosomal protein L7a binds RNA through two distinct RNA-binding domains

Cited by 31 publications

References 31 publications

Human rpL3 induces G₁/S arrest or apoptosis by modulating p21waf1/cip1 levels in a p53-independent manner

Human rpL3 induces G₁/S arrest or apoptosis by modulating p21waf1/cip1 levels in a p53-independent manner

Unusual features of fibrillarin cDNA and gene structure in Euglena gracilis: evolutionary conservation of core proteins and structural predictions for methylation-guide box C/D snoRNPs throughout the domain Eucarya

rpL3 promotes the apoptosis of p53 mutated lung cancer cells by down-regulating CBS and NFκB upon 5-FU treatment

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Human rpL3 induces G₁/S arrest or apoptosis by modulating p21^waf1/cip1 levels in a p53-independent manner

Human rpL3 induces G₁/S arrest or apoptosis by modulating p21^waf1/cip1 levels in a p53-independent manner