Ribosomal Protein Rps26 Influences 80S Ribosome Assembly in Saccharomyces cerevisiae

Belyy, Alexander; Levanova, Nadezhda; Tabakova, Irina; Rospert, Sabine; Belyi, Yury

doi:10.1128/msphere.00109-15

Cited by 9 publications

(10 citation statements)

References 58 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RPS9 can interact with nucleolar protein and facilitate ribosome biogenesis 38 . RPS26 interacts with the 5′ untranslated region of mRNA and might play an important role in translation initiation 39 . Notably, the function of RPS6 is linked to mTOR, which exerts signalling effects associated with cell size, cell proliferation, and glucose homeostasis 40 .…”

Section: Discussionmentioning

confidence: 99%

Complementary transcriptomic and proteomic analyses reveal regulatory mechanisms of milk protein production in dairy cows consuming different forages

Dai

Chen

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Forage plays a critical role in the milk production of dairy cows; however, the mechanisms regulating bovine milk synthesis in dairy cows fed high forage rations with different basal forage types are not well-understood. In the study, rice straw (RS, low-quality) and alfalfa hay (AH, high-quality) diets were fed to lactating cows to explore how forage quality affected the molecular mechanisms regulating milk production using RNA-seq transcriptomic method with iTRAQ proteomic technique. A total of 554 transcripts (423 increased and 131 decreased) and 517 proteins (231 up-regulated and 286 down-regulated) were differentially expressed in the mammary glands of the two groups. The correlation analysis demonstrated seven proteins (six up-regulated and one down-regulated) had consistent mRNA expression. Functional analysis of the differentially expressed transcripts/proteins suggested that enhanced capacity for energy and fatty acid metabolism, increased protein degradation, reduced protein synthesis, decreased amino acid metabolism and depressed cell growth were related to RS consumption. The results indicated cows consuming RS diets may have had depressed milk protein synthesis because these animals had decreased capacity for protein synthesis, enhanced proteolysis, inefficient energy generation and reduced cell growth. Additional work evaluating RS- and AH-based rations may help better isolate molecular adaptations to low nutrient availability during lactation.

show abstract

Section: Discussionmentioning

confidence: 99%

Complementary transcriptomic and proteomic analyses reveal regulatory mechanisms of milk protein production in dairy cows consuming different forages

Dai

Chen

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The rise of these transcripts appears to be necessary to supply the demands for making functional ribosome precursors, such as SSU and LSU, which consist of rRNA and its binding partners, ribosomal proteins. After transportation to the cytoplasm with the aid of nucleoporins, such as Nup2 [43] and Pom152 [44], the SSU and LSU join together along with tRNAs to make a translation-competent supramolecular complex that manufactures proteins de novo to replace nonfunctional proteins that might have been damaged by exposure to ROS [45,46]. In addition to increasing the rate of ribosomal production, we also noticed genes involved in tRNA maturation to be significantly increased to provide additional amino acid products required in translation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profile Alteration with Cadmium Selenide/Zinc Sulfide Quantum Dots in Saccharomyces cerevisiae

Horstmann

Kim²,

Campbell

et al. 2019

Biomolecules

View full text Add to dashboard Cite

Quantum Dots (QDs) are becoming more prevalent in products used in our daily lives, such as TVs and laptops, due to their unique and tunable optical properties. The possibility of using QDs as fluorescent probes in applications, such as medical imaging, has been a topic of interest for some time, but their potential toxicity and long-term effects on the environment are not well understood. In the present study, we investigated the effects of yellow CdSe/ZnS-QDs on Saccharomyces cerevisiae. We utilized growth assays, RNA-seq, reactive oxygen species (ROS) detection assays, and cell wall stability experiments to investigate the potential toxic effects of CdSe/ZnS-QDs. We found CdSe/ZnS-QDs had no negative effects on cell viability; however, cell wall-compromised cells showed more sensitivity in the presence of 10 µg/mL CdSe/ZnS-QDs compared to non-treated cells. In CdSe/ZnS-treated and non-treated cells, no significant change in superoxide was detected, but according to our transcriptomic analysis, thousands of genes in CdSe/ZnS-treated cells became differentially expressed. Four significantly differentiated genes found, including FAF1, SDA1, DAN1, and TIR1, were validated by consistent results with RT-qPCR assays. Our transcriptome analysis led us to conclude that exposure of CdSe/ZnS-QDs on yeast significantly affected genes implicated in multiple cellular processes.

show abstract

“…RPS26 and SUOX were both identified as significant mediators in multiple tissues in the GTEx dataset; however, within the same tissue, the trans-associations mediated through SUOX were not identical to those through RPS26, indicating distinct effects of SUOX and RPS26 on distant genes 36 . RPS26 encodes a ribosomal subunit protein which, apart from its role in ribosome assembly and translation 37,38 , is involved in various other cellular processes, including nonsense-mediated mRNA decay 39 and p53 transcriptional activity 40 . It is likely that the broad trans effects of RPS26 are related to its ribosomal functions.…”

Section: Discussionmentioning

confidence: 99%

Neonatal genetics of gene expression reveal the origins of autoimmune and allergic disease risk

Huang

Tang

Teo

et al. 2019

Preprint

View full text Add to dashboard Cite

Chronic immune-mediated diseases of adulthood often originate in early childhood. To investigate genetic associations between neonatal immunity and disease, we collected cord blood samples from a birth cohort and mapped expression quantitative trait loci (eQTLs) in resting monocytes and CD4 + T cells as well as in response to lipopolysaccharide (LPS) or phytohemagglutinin (PHA) stimulation, respectively. Cis-eQTLs were largely specific to cell type or stimulation, and response eQTLs were identified for 31% of genes with cis-eQTLs (eGenes) in monocytes and 52% of eGenes in CD4 + T cells.We identified trans-eQTLs and mapped cis regulatory factors which act as mediators of trans effects.There was extensive colocalisation of causal variants for cell type-and stimulation-specific neonatal cis-eQTLs and those of autoimmune and allergic diseases, in particular CTSH (Cathepsin H) which showed widespread colocalisation across diseases. Mendelian randomisation showed causal neonatal gene transcription effects on disease risk for BTN3A2, HLA-C and many other genes. Our study elucidates the genetics of gene expression in neonatal conditions and cell types as well as the aetiological origins of autoimmune and allergic diseases.

show abstract

Ribosomal Protein Rps26 Influences 80S Ribosome Assembly in Saccharomyces cerevisiae

Cited by 9 publications

References 58 publications

Complementary transcriptomic and proteomic analyses reveal regulatory mechanisms of milk protein production in dairy cows consuming different forages

Complementary transcriptomic and proteomic analyses reveal regulatory mechanisms of milk protein production in dairy cows consuming different forages

Transcriptome Profile Alteration with Cadmium Selenide/Zinc Sulfide Quantum Dots in Saccharomyces cerevisiae

Neonatal genetics of gene expression reveal the origins of autoimmune and allergic disease risk

Contact Info

Product

Resources

About