Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray

McGowan, Sarah; Nuñéz-García, Javier; Steinbach, Falko; Rocca, Anna; Blake, Damer P.; Dastjerdi, Akbar

doi:10.1016/j.jviromet.2014.07.012

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…A stock for inoculations was grown in primary porcine peripheral blood monocytes and viral titres were determined as the amount of virus causing haemadsorption in 50% of inoculated cultures (HAD 50 /ml). Freedom from other porcine pathogens was confirmed by analysis of viral nucleic acid extracted from stocks on a pan viral microarray 37 .…”

Section: Methodsmentioning

confidence: 99%

Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus

McCleary

Strong

McCarthy

et al. 2020

Sci Rep

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African swine fever virus (ASFV) causes a lethal, haemorrhagic disease in domestic swine that threatens pig production across the globe. Unlike domestic pigs, warthogs, which are wildlife hosts of the virus, do not succumb to the lethal effects of infection. There are three amino acid differences between the sequence of the warthog and domestic pig ReLA protein; a subunit of the nf-κB transcription factor that plays a key role in regulating the immune response to infections. Domestic pigs with all 3 or 2 of the amino acids from the warthog RELA orthologue have been generated by gene editing. To assess if these variations confer resilience to ASF we established an intranasal challenge model with a moderately virulent ASFV. No difference in clinical, virological or pathological parameters were observed in domestic pigs with the 2 amino acid substitution. Domestic pigs with all 3 amino acids found in warthog RELA were not resilient to ASF but a delay in onset of clinical signs and less viral DNA in blood samples and nasal secretions was observed in some animals. Inclusion of these and additional warthog genetic traits into domestic pigs may be one way to assist in combating the devastating impact of ASFV. African swine fever virus (ASFV) is a large DNA virus and sole member of the family Asfarviridae that causes a mostly lethal haemorrhagic disease, African swine fever (ASF), in domestic pigs and Eurasian wild boar. ASFV can genetically be separated into 24 genotypes that cause the same disease, but immunological cross-protection is limited and poorly understood 1. The introduction of ASF into a country results in trade restrictions and pig losses, thus the disease has a high socioeconomic consequence for both commercial and backyard farmers 2. Accordingly, the spread of this disease is a serious concern for the global pig industry. Following the incursion of a genotype II ASFV into the Caucasus in 2007 the virus has spread through Russia, entered the European Union in 2014 and, in 2018, was detected for the first time in China. Since then the Chinese pig population has declined by at least 20% and ASFV has further spread across many countries in South East Asia 3,4. Combating this global threat is hampered by the lack of a vaccine and is particularly difficult in production systems with poor biosecurity which are more vulnerable to virus introduction and contact with wild suids 1. ASFV infects all members of the family Suidae, which as well as domestic pigs (Sus scrofa domesticus), wild boar (Sus scrofa spp) and others also includes bushpigs (Potamochoerus spp.) and warthogs (Phacochoerus spp.), which are considered natural reservoir hosts 5. In domestic pigs and wild boar acute and subacute forms dominate,

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Section: Methodsmentioning

confidence: 99%

Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus

McCleary

Strong

McCarthy

et al. 2020

Sci Rep

Self Cite

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“…The presence of secondary infections in our in vivo animal model was minimised by sourcing animals from a farm free of the two main bacterial co-pathogens in this syndrome, namely Mycoplasma hyopneumoniae and Haemophilus parasuis (Thacker et al, 1999;Gómez-Laguna et al, 2013b). While a co-infection cannot be ruled out on this basis, all tests for secondary pathogens at the post mortems, including Mycoplasma hyopneumoniae and viral agents with the use of a panviral microarray as previously described (McGowan et al, 2014) were negative.…”

Section: Clinicopathological Outcomes After Prrsv-1 Respiratory Infecmentioning

confidence: 99%

Host–pathogen interactions during porcine reproductive and respiratory syndrome virus 1 infection of piglets

Salguero

Frossard

Rebel³

et al. 2015

Virus Research

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Porcine reproductive and respiratory syndrome (PRRS) is a major disease affecting pigs worldwide and resulting in considerable economic losses. While PRRS is a global phenomenon, the causative viruses PRRSV-1 (first detected in Europe) and PRRSV-2 (isolated in North America) are genetically and biologically distinct. In addition, the disease outcome is directly linked to co-infections associated with the porcine respiratory disease complex and the host response is variable between different breeds of pigs. It is therefore warranted when studying the pathogenesis of PRRS to consider each viral genotype separately and apply careful consideration to the disease model studied. We here review the respiratory pig model for PRRSV-1, with a focus on a recent set of studies conducted with carefully selected virus strains and pigs, which may serve as both a baseline and benchmark for future investigation.

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Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray

Cited by 2 publications

References 17 publications

Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus

Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus

Host–pathogen interactions during porcine reproductive and respiratory syndrome virus 1 infection of piglets

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