Rice APOPTOSIS INHIBITOR5 Coupled with Two DEAD-Box Adenosine 5′-Triphosphate-Dependent RNA Helicases Regulates Tapetum Degeneration

Li, Xingwang; Gao, Xinqiang; Wei, Yangyang; Deng, Li; Ouyang, Yidan; Chen, Guoxing; Li, Xianghua; Zhang, Qifa; Wu, Changyin

doi:10.1105/tpc.110.082636

Cited by 131 publications

(114 citation statements)

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“…S4a-f). OsCP1, a cysteine protease-encoding gene 40 that is directly regulated by TDR and API5 11,13 , exhibited a slight reduction in expression until stage 10, and an increase in expression after stage 11 ( Supplementary Fig. S4g).…”

Section: Eat1 Acts Downstream From and Interacts With Tdrmentioning

confidence: 99%

“…Several transcriptional regulators have been reported to be associated with tapetal degeneration, including Arabidopsis MYB33/MYB65 4 , DYSFUNCTIONAL TAPETUM1 (DYT1) 5 , DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION1 (TDF1) 6 , ABORTED MICROSPORE (AMS) 7 , MALE STERILITY1 8 , rice GAMYB 9 , UNDEVELOPED TAPETUM1 10 , TAPETUM DEGENERATION RETARDATION (TDR) 11 , PERSISTENT TAPETAL CELL 1 (PTC1) 12 , and APOPTOSIS INHIBITOR5 (API5) 13 . However, mechanisms by which these regulators regulate tapetal PCD remain unclear.…”

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confidence: 99%

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EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

et al. 2013

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Programmed cell death is essential for the development of multicellular organisms, yet pathways of plant programmed cell death and its regulation remain elusive. Here we report that ETERNAL TAPETUM 1, a basic helix-loop-helix transcription factor conserved in land plants, positively regulates programmed cell death in tapetal cells in rice anthers. eat1 exhibits delayed tapetal cell death and aborted pollen formation. ETERNAL TAPETUM 1 directly regulates the expression of OsAP25 and OsAP37, which encode aspartic proteases that induce programmed cell death in both yeast and plants. Expression and genetic analyses revealed that ETERNAL TAPETUM 1 acts downstream of TAPETUM DEGENERATION RETARDATION, another positive regulator of tapetal programmed cell death, and that ETERNAL TAPETUM 1 can also interact with the TAPETUM DEGENERATION RETARDATION protein. This study demonstrates that ETERNAL TAPETUM 1 promotes aspartic proteases triggering plant programmed cell death, and reveals a dynamic regulatory cascade in male reproductive development in rice.

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Section: Eat1 Acts Downstream From and Interacts With Tdrmentioning

confidence: 99%

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confidence: 99%

EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

et al. 2013

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“…Therefore, the mutation in osasmR5864m reduces the degree of its matching to these nine candidate targets. A number of anther development-related genes have been cloned in rice, including Gibberellins MYB gene (GAMYB) [54,55], Undeveloped Tapetum1 (UDT1) [56], Tapetum Degeneration Retardation gene (TDR) [57], CYP704B2 [58], OsC6 [59], MADS3 [60], Carbon Starved Anther (CSA) [61], APOPTOSIS (API5) [62] and MEIOSIS ARRESTED AT LEPTOTENE1 (MEL1) [63]. These genes were not recognized as the potential targets of osa-smR5864w as searched by the CSRDB tool.…”

Section: Wwwcell-researchcom | Cell Research Hai Zhou Et Al 655mentioning

confidence: 99%

Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

et al. 2012

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Photoperiod-and thermo-sensitive genic male sterility (PGMS and TGMS) are the core components for hybrid breeding in crops. Hybrid rice based on the two-line system using PGMS and TGMS lines has been successfully developed and applied widely in agriculture. However, the molecular mechanism underlying the control of PGMS and TGMS remains obscure. In this study, we mapped and cloned a major locus, p/tms12-1 (photo-or thermo-sensitive genic male sterility locus on chromosome 12), which confers PGMS in the japonica rice line Nongken 58S (NK58S) and TGMS in the indica rice line Peiai 64S (PA64S, derived from NK58S). A 2.4-kb DNA fragment containing the wild-type allele P/TMS12-1 was able to restore the pollen fertility of NK58S and PA64S plants in genetic complementation. P/TMS12-1 encodes a unique noncoding RNA, which produces a 21-nucleotide small RNA that we named osa-smR5864w. A substitution of C-to-G in p/tms12-1, the only polymorphism relative to P/TMS12-1, is present in the mutant small RNA, namely osa-smR5864m. Furthermore, overexpression of a 375-bp sequence of P/TMS12-1 in transgenic NK58S and PA64S plants also produced osa-smR5864w and restored pollen fertility. The small RNA was expressed preferentially in young panicles, but its expression was not markedly affected by different day lengths or temperatures. Our results reveal that the point mutation in p/tms12-1, which probably leads to a loss-of-function for osa-smR5864m, constitutes a common cause for PGMS and TGMS in the japonica and indica lines, respectively. Our findings thus suggest that this noncoding small RNA gene is an important regulator of male development controlled by cross-talk between the genetic networks and environmental conditions. Keywords: rice; PGMS; TGMS; noncoding RNA; small RNA Cell Research (2012) IntroductionAsian cultivated rice (Oryza sativa L.) is one of the world's most important crops, meeting the stable food demand of more than half of the global population. The development of hybrid rice is a major approach for increasing the yield potential of rice, since hybrid rice varieties have about 20% or more yield advantage over improved inbred varieties [1]. Hybrid rice technologies are mainly based on the three-line and two-line systems. The three-line system uses cytoplasmic male sterility lines, maintainer lines and restorer lines [2,3]. The twoline hybrid rice is based on the discovery and application of environmentally sensitive genic male sterile (EGMS) lines, which serve as both the male sterile lines and maintainer lines under different environmental conditions [4]. Thus, the two-line hybrid rice system is an important innovation for the better exploitation of hybrid vigor (heterosis). Compared with the three-line system, the advantages of the two-line system include a wider range Rice PGMS and TGMS caused by a point mutation in a noncoding RNA 650 of germplasm resources used as breeding parents, better grain quality and higher yields, and benefiting from simpler procedures for breeding and hybrid seed production [5...

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“…API5 (apoptosis inhibitor 5), also known as AAC-11 protein (anti-apoptosis clone 11), FIF (fibroblast growth factor 2-interacting factor), and MIG8 (cell migration-inducing gene 8), is a relatively poorly studied apoptosis-inhibiting nuclear protein that does not contain a baculovirus IAP repeat domain. The API5 gene has been found in animals, protists, and plants (13). The expression of API5 prevents apoptosis following growth factor deprivation, and this protein is up-regulated in various cancer cells (14 -19).…”

Section: Apoptosis Inhibitor 5 (Api5) Is An Anti-apoptotic Protein Thmentioning

confidence: 99%

Helical Repeat Structure of Apoptosis Inhibitor 5 Reveals Protein-Protein Interaction Modules

Han

Kim

Lee

et al. 2012

Journal of Biological Chemistry

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Background: Up-regulated in various cancers, API5 prevents apoptosis under growth factor deprivation. Results: We have determined the crystal structure of API5 with the HEAT and ARM repeat and show that Lys-251 acetylation is important for its function. Conclusion: API5 likely serves as a scaffold for multiprotein complex with its cellular function regulated by lysine acetylation. Significance: Structural basis of API5 function is important in targeting anti-apoptosis.

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Rice APOPTOSIS INHIBITOR5 Coupled with Two DEAD-Box Adenosine 5′-Triphosphate-Dependent RNA Helicases Regulates Tapetum Degeneration

Cited by 131 publications

References 58 publications

EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

Helical Repeat Structure of Apoptosis Inhibitor 5 Reveals Protein-Protein Interaction Modules

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