Rice Blast in India and Strategies to Develop Durably Resistant Cultivars

Variar, M.; Cruz, C. M. Vera; Carrillo, María Gricelda Vázquez; Bhatt, J. C.; Sangar, R. B. S.

doi:10.1007/978-1-4020-9500-9_35

Cited by 28 publications

(13 citation statements)

References 13 publications

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“…The field survival reaction of the gene pyramided NILs was proved very effective against blast disease at UBN at Hazaribagh and Malan, located respectively at eastern and north-western India. Among the genes pyramided, efficacy of gene Pi2 in the eastern and northern parts of the country has already been reported [20,68,69]. Multiple lineages consisting of multiple pathotypes in each of the lineages have been reported [70].…”

Section: Discussionmentioning

confidence: 99%

Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding

et al. 2016

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Marker assisted backcross breeding was employed to incorporate the blast resistance genes, Pi2 and Pi54 and bacterial blight (BB) resistance genes xa13 and Xa21 into the genetic background of Pusa Basmati 1121 (PB1121) and Pusa Basmati 6. Foreground selection for target gene(s) was followed by arduous phenotypic and background selection which fast-tracked the recovery of recurrent parent genome (RPG) to an extent of 95.8% in one of the near-isogenic lines (NILs) namely, Pusa 1728-23-33-31-56, which also showed high degree of resemblance to recurrent parent, PB6 in phenotype. The phenotypic selection prior to background selection provided an additional opportunity for identifying the novel recombinants viz., Pusa 1884-9-12-14 and Pusa 1884-3-9-175, superior to parental lines in terms of early maturity, higher yield and improved quality parameters. There was no significant difference between the RPG recovery estimated based on SSR or SNP markers, however, the panel of SNPs markers was considered as the better choice for background selection as it provided better genome coverage and included SNPs in the genic regions. Multi-location evaluation of NILs depicted their stable and high mean performance in comparison to the respective recurrent parents. The Pi2+Pi54 carrying NILs were effective in combating a pan-India panel of Magnaporthe oryzae isolates with high level of field resistance in northern, eastern and southern parts of India. Alongside, the PB1121-NILs and PB6-NILs carrying BB resistance genes xa13+Xa21 were resistant against Xanthomonas oryzae pv. oryzae races of north-western, southern and eastern parts of the country. Three of NILs developed in this study, have been promoted to final stage of testing during the Kharif 2015 in the Indian National Basmati Trial.

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Section: Discussionmentioning

confidence: 99%

Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding

et al. 2016

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“…Among the biotic stresses, blast disease is the mostdestructive disease in rice [3]. Still now, developing blastresistant varieties is the most cost-effective method to improve rice blast disease resistance in rice [4].…”

Section: Introductionmentioning

confidence: 99%

Recurrent parent genome recovery analysis in a marker-assisted backcrossing program of rice (Oryza sativa L.)

Miah

Rafii

Ismail

et al. 2014

Comptes Rendus. Biologies

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“…In this study, polymorphism of the Pi9 allele was investigated in seven rice landraces. Earlier study about the prevalence of high pathotypic diversity of the M. oryzae population of Eastern India and very rare compatibility of Pi9 gene in field evaluations with isolates from this region result in considering Pi9 gene further in rice landraces (Variar et al, 2009). Our earlier results of virulence analysis of 72 M. oryzae isolates against 26 differential variety revealed that matching virulence to all monogenic differentials carrying different resistant genes were present in the pathogen population, although the resistant check Tetep was resistant to all of them.…”

Section: Discussionmentioning

confidence: 99%

“…The genome structure at Pi9 locus is highly conserved but the LRR region showed high sequence variation giving rise to positive selection for Pi9 genes among the rice germplasm (Zhou et al, 2007; Dai et al, 2010; Liu et al, 2010). Previous data toward the prevalence of high pathotypic diversity of the M. oryzae population from Eastern India and very rare compatibility of Pi9 gene in field evaluations with isolates from this region provoked this study (Variar et al, 2009). Amongst the multi-genes near the waxy gene locus on chromosome 6, Pi9 was extra efficient than Pi2 ( Piz-t ) in the preceding investigation (Ballini et al, 2008; Imam et al, 2014b).…”

Section: Introductionmentioning

confidence: 99%

Allele Mining and Selective Patterns of Pi9 Gene in a Set of Rice Landraces from India

et al. 2016

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Allelic variants of the broad-spectrum blast resistance gene, Pi9 (nucleotide binding site-leucine-rich repeat region) have been analyzed in Indian rice landraces. They were selected from the list of 338 rice landraces phenotyped in the rice blast nursery at central Rainfed Upland Rice Research Station, Hazaribag. Six of them were further selected on the basis of their resistance and susceptible pattern for virulence analysis and selective pattern study of Pi9 gene. The sequence analysis and phylogenetic study illustrated that such sequences are vastly homologous and clustered into two groups. All the blast resistance Pi9 alleles were grouped into one cluster, whereas Pi9 alleles of susceptible landraces formed another cluster even though these landraces have a low level of DNA polymorphisms. A total number of 136 polymorphic sites comprising of transitions, transversions, and insertion and deletions (InDels) were identified in the 2.9 kb sequence of Pi9 alleles. Lower variation in the form of mutations (77) (Transition + Transversion), and InDels (59) were observed in the Pi9 alleles isolated from rice landraces studied. The results showed that the Pi9 alleles of the selected rice landraces were less variable, suggesting that the rice landraces would have been exposed to less number of pathotypes across the country. The positive Tajima’s D (0.33580), P > 0.10 (not significant) was observed among the seven rice landraces, which suggests the balancing selection of Pi9 alleles. The value of synonymous substitution (-0.43337) was less than the non-synonymous substitution (0.78808). The greater non-synonymous substitution than the synonymous means that the coding region, mainly the leucine-rich repeat domain was under diversified selection. In this study, the Pi9 gene has been subjected to balancing selection with low nucleotide diversity which is different from the earlier reports, this may be because of the closeness of the rice landraces, cultivated in the same region, and under low pathotype pressure.

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Rice Blast in India and Strategies to Develop Durably Resistant Cultivars

Cited by 28 publications

References 13 publications

Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding

Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding

Recurrent parent genome recovery analysis in a marker-assisted backcrossing program of rice (Oryza sativa L.)

Allele Mining and Selective Patterns of Pi9 Gene in a Set of Rice Landraces from India

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