Rice inositol polyphosphate kinase gene (OsIPK2), a putative new player of gibberellic acid signaling, involves in modulation of shoot elongation and fertility

Chen, Yao; Wei, Zhaoyun; Yang, Qiaofeng; Sang, Sihong; Wang, Peng

doi:10.1007/s11240-017-1298-0

Cited by 5 publications

(3 citation statements)

References 51 publications

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“…Yeast Ipk2 (IP3K/Arg82) is involved in cellular mRNA export from the nucleus with Ipk1 and controls arginine-dependent gene expression 63 . Plant AtIPK2 and OsIPK2 play a role in gibberellin and auxin signaling pathway, respectively 64 , 65 . In addition, PI-PLCs were also reported to function in signal transduction.…”

Section: Discussionmentioning

confidence: 99%

A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation

Xu,

Cui,

et al. 2024

Nat Commun

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Inositol hexaphosphate (InsP6) is the major storage form of phosphorus in seeds. Reducing seed InsP6 content is a breeding objective in agriculture, as InsP6 negatively impacts animal nutrition and the environment. Nevertheless, how InsP6 accumulation is regulated remains largely unknown. Here, we identify a clade of receptor-like cytoplasmic kinases (RLCKs), named Inositol Polyphosphate-related Cytoplasmic Kinases 1-6 (IPCK1-IPCK6), deeply involved in InsP6 accumulation. The InsP6 concentration is dramatically reduced in seeds of ipck quadruple (T-4m/C-4m) and quintuple (C-5m) mutants, accompanied with the obviously increase of phosphate (Pi) concentration. The plasma membrane-localized IPCKs recruit IPK1 involved in InsP6 synthesis, and facilitate its binding and activity via phosphorylation of GRF 14-3-3 proteins. IPCKs also recruit IPK2s and PI-PLCs required for InsP4/InsP5 and InsP3 biosynthesis respectively, to form a potential IPCK-GRF-PLC-IPK2-IPK1 complex. Our findings therefore uncover a regulatory mechanism of InsP6 accumulation governed by IPCKs, shedding light on the mechanisms of InsP biosynthesis in eukaryotes.

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Section: Discussionmentioning

confidence: 99%

A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation

Xu,

Cui,

et al. 2024

Nat Commun

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“…However, their physiological functions have not been reported. Recently, it was reported that inositol polyphosphate kinase directly interacts with OsIAA11 to regulate lateral root formation and was involved in gibberellic acid signaling modulation to affect shoot elongation and fertility [ 76 , 77 ]. In this study, different from previously reported genes, the early senescence phenotype was caused by a mutation in the ES2 / OsIPK2 gene for inositol polyphosphate kinase.…”

Section: Discussionmentioning

confidence: 99%

Rice EARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence

et al. 2020

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Background Early leaf senescence influences yield and yield quality by affecting plant growth and development. A series of leaf senescence-associated molecular mechanisms have been reported in rice. However, the complex genetic regulatory networks that control leaf senescence need to be elucidated. Results In this study, an early senescence 2 ( es2 ) mutant was obtained from ethyl methanesulfonate mutagenesis (EMS)-induced mutational library for the Japonica rice cultivar Wuyugeng 7 (WYG7). Leaves of es2 showed early senescence at the seedling stage and became severe at the tillering stage. The contents of reactive oxygen species (ROS) significantly increased, while chlorophyll content, photosynthetic rate, catalase (CAT) activity significantly decreased in the es2 mutant. Moreover, genes which related to senescence, ROS and chlorophyll degradation were up-regulated, while those associated with photosynthesis and chlorophyll synthesis were down-regulated in es2 mutant compared to WYG7. The ES2 gene, which encodes an inositol polyphosphate kinase (OsIPK2), was fine mapped to a 116.73-kb region on chromosome 2. DNA sequencing of ES2 in the mutant revealed a missense mutation, ES2 was localized to nucleus and plasma membrane of cells, and expressed in various tissues of rice. Complementation test and overexpression experiment confirmed that ES2 completely restored the normal phenotype, with chlorophyll contents and photosynthetic rate increased comparable with the wild type. These results reveal the new role of OsIPK2 in regulating leaf senescence in rice and therefore will provide additional genetic evidence on the molecular mechanisms controlling early leaf senescence. Conclusions The ES2 gene, encoding an inositol polyphosphate kinase localized in the nucleus and plasma membrane of cells, is essential for leaf senescence in rice. Further study of ES2 will facilitate the dissection of the genetic mechanisms underlying early leaf senescence and plant growth.

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“…The effects of InsPs as signalling molecules are based on the specificity of their interactions [63]. PP-InsPs are signalling molecules in auxin, jasmonate, gibberellin and phosphate signalling pathways, where varying degrees of phosphorylation have been associated with divergent downstream functions [57,60,64,65]. One prerequisite for PP-InsPs signalling is their low abundance with cellular InsP 7 concentrations of 1-5 mM and InsP 8 concentrations ∼10-20% of those of InsP 7 [66].…”

Section: Signalling Of Plant Phosphorus Status Must Be Specific and D...mentioning

confidence: 99%

Dynamic interactions between SPX proteins, the ubiquitination machinery, and signalling molecules for stress adaptation at a whole-plant level

Collins,

Shou,

Mao

et al. 2024

Biochemical Journal

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The plant macronutrient phosphorus is a scarce resource and plant-available phosphate is limiting in most soil types. Generally, a gene regulatory module called the phosphate starvation response (PSR) enables efficient phosphate acquisition by roots and translocation to other organs. Plants growing on moderate to nutrient-rich soils need to co-ordinate availability of different nutrients and repress the highly efficient PSR to adjust phosphate acquisition to the availability of other macro- and micronutrients, and in particular nitrogen. PSR repression is mediated by a small family of single SYG1/Pho81/XPR1 (SPX) domain proteins. The SPX domain binds higher order inositol pyrophosphates that signal cellular phosphorus status and modulate SPX protein interaction with PHOSPHATE STARVATION RESPONSE1 (PHR1), the central transcriptional regulator of PSR. Sequestration by SPX repressors restricts PHR1 access to PSR gene promoters. Here we focus on SPX4 that primarily acts in shoots and sequesters many transcription factors other than PHR1 in the cytosol to control processes beyond the classical PSR, such as nitrate, auxin, and jasmonic acid signalling. Unlike SPX1 and SPX2, SPX4 is subject to proteasomal degradation not only by singular E3 ligases, but also by SCF–CRL complexes. Emerging models for these different layers of control and their consequences for plant acclimation to the environment will be discussed.

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Rice inositol polyphosphate kinase gene (OsIPK2), a putative new player of gibberellic acid signaling, involves in modulation of shoot elongation and fertility

Cited by 5 publications

References 51 publications

A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation

A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation

Rice EARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence

Dynamic interactions between SPX proteins, the ubiquitination machinery, and signalling molecules for stress adaptation at a whole-plant level

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