Rice lipid transfer protein (LTP) genes belong to a complex multigene family and are differentially regulated

Vignols, Florence; Wigger, Maria; García‐Garrido, José Manuel; Grellet, Françoise; Kader, Jean‐Claude; Delseny, Michel

doi:10.1016/s0378-1119(97)00137-6

Cited by 66 publications

(53 citation statements)

References 23 publications

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“…Three (AU164106, AU092028 and AU090552) of the seven genes were very similar to known genes with unclear function. AU164106 was very similar to the rice lipid transfer protein b21 gene (Ltp b21) (BLASTN E-value = e-103) whose expression was reported in the mesocotyl and root (Vignols et al 1997). On the other hand, AU092028 and AU090552 were very similar to the gene for wheat peptidylprolyl isomerase FKBP77 (EC 5.2.1.8) (BLASTX E-value = 7e-35) displaying a heat shock-induced expression (Kurek et al 1999), and the gene for rice chitinase IIb (EC 3.2.1.14) (BLASTX E-value = 5e-72) identified in rice husks as a candidate for dormancy-related protein (Nakazaki and Ikehashi 1998), respectively.…”

Section: Unclassified Genesmentioning

confidence: 54%

Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths

et al. 2005

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In rice (Oryza sativa L.) plants, the leaf sheaths of the upper leaves accumulate a large amount of starch before heading, and the accumulated starch is converted to sucrose and translocated to the panicles after heading. To analyze the regulation of sink-source transition, we carried out large-scale monitoring of gene expression by microarray analysis using 8987 rice expressed sequence tags (ESTs), and identified 102 developmentally regulated genes in the leaf sheaths of the first leaves below the flag leaves during the heading period. Identified genes included multiple genes related to starch biosynthesis, cell division and expansion, and photosynthesis. All of them showed early stage-preferential expression, probably reflecting the decrease of starch biosynthesis, end of elongation and decrease of photosynthesis in the leaf sheaths during the heading period, respectively. The expression patterns of the genes for starch biosynthesis enzymes and α-tubulin suggest that the leaf sheaths displayed both accumulating-and consuming-sink functions at the very early stage of the heading period. Northern blot analysis of the genes for starch degradation and sucrose biosynthesis enzymes (α-amylase3D and sucrose phosphate synthase) revealed that the induction of the genes did not occur when the starch amount began to decrease, suggesting that the mechanisms of starch degradation and sucrose re-synthesis in the leaf sheaths during the heading period were different from those in germinating seeds. Furthermore, we identified 18 developmentally regulated genes whose functions in the leaf sheaths are unknown. They included seven genes those were preferentially expressed in the bottom part of the leaf sheaths, implying that they were involved in functions related to the starch metabolism. The results obtained showed the effectiveness of the microarray technique to analyze complex and uncharacterized phenomena.

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Section: Unclassified Genesmentioning

confidence: 54%

Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths

et al. 2005

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“…At least one member of the NgLTP gene family is upregulated with drought stress, increased cuticular wax deposition results from periodic dehydration stress, and we observed increased accumulation of NgLTP transcripts in plants with greater deposition of cuticular wax. Individual members of the LTP families are up-and/or down-regulated under dehydration stress in wild tomato (Lycopersicon pennellii), rice, and pepper (Capsicum annuum; Vignols et al, 1997;Treviñ o and O'Connell, 1998;Jung et al, 2003). Due to our inability to resolve the expression patterns of the five known NgLTP genes at this time, neither down-regulation nor functional redundancy within the NgLTP gene family under dehydration stress has been examined, though it is known that NgLTP genes are differentially regulated due to other environmental factors.…”

Section: Discussionmentioning

confidence: 99%

Increased Accumulation of Cuticular Wax and Expression of Lipid Transfer Protein in Response to Periodic Drying Events in Leaves of Tree Tobacco

2005

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Cuticular wax deposition and composition affects drought tolerance and yield in plants. We examined the relationship between wax and dehydration stress by characterizing the leaf cuticular wax of tree tobacco (Nicotiana glauca L. Graham) grown under periodic dehydration stress. Total leaf cuticular wax load increased after each of three periods of dehydration stress using a CH 2 Cl 2 extraction process. Overall, total wax load increased 1.5-to 2.5-fold, but composition of the wax was not altered. Homologous series of wax components were classified into organic groups; n-hentriacontane was the largest component (.75%) with alcohols and fatty acids representing ,10% of the entire wax load. An increase in density, but no change in the three-dimensional shape, of leaf wax crystals was evident under low-kV scanning electron microscopy after each drying event. Leaves excised from plants subjected to multiple drying events were more resistant to water loss compared to leaves excised from well-watered plants, indicating that there is a negative relationship between total wax load and epidermal conductance. Lipid transfer proteins (LTPs) are thought to be involved in the transfer of lipids through the extracellular matrix for the formation of cuticular wax. Using northern analysis, a 6-fold increase of tree tobacco LTP gene transcripts was observed after three drying events, providing further evidence that LTP is involved in cuticle deposition. The simplicity of wax composition and the dramatic wax bloom displayed by tree tobacco make this an excellent species in which to study the relationship between leaf wax deposition and drought tolerance.

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“…Зо-крема, нами було показано, що під дією СК і ЯК підвищується рівень експресії гена дефензину сосни (PsDef1) [3]. З літератури відомо, що ЯК індукує експресію LTP у арабідопсису, рису, перцю, пшениці, капусти, льону, а СК, у свою чергу, мала здат-ність індукувати експресію LTP у рису, арабідопсису, тютюну та льону [4,12,13,25,26,28]. Протилежний ефект дії цих гормонів на рівень експресії ліпідтрансферного протеїну ми спостерігали після обприскування ними проростків сосни.…”

Section: Rpl44unclassified

Patterns of expression of lipid-transfer protein gene in organs of Scots pine (Pinus sylvestris L.)

2012

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Визначено профіль експресії гена ліпідтрансферного протеїну PsLTP1 (Pinus sylvestris lipid transfer protein 1) у вегетативних та генеративних органах сосни зви-чайної (Pinus sylvestris L.) методом полімеразно-ланцюгової реакції зі зворотною транскрипцією (ЗТ-ПЛР). Встановлено, що експресія гена PsLTP1 є тканиноспеци-фічною та змінюється протягом онтогенетичного розвитку. Досліджено вплив фіто-гормонів на рівень експресії PsLTP1 у проростках сосни звичайної.Ключові слова: сосна звичайна, ліпідтрансферний протеїн PsLTP1, експресія. ВСТУПУ ході еволюції рослинні організми розвинули багаторівневу систему захисту від потенційних патогенів. Одним із компонентів цієї системи є велика група сполук із антимікробними властивостями: фітоалексини, фітоантисипіни, протеїни, пов'я-зані з патогенезом (PR-протеїни) і антимікробні пептиди (АМП). За просторовою структурою та характерним розташуванням залишків цистеїну в молекулі АМП рослин поділяються на декілька родин: дефензини, тіоніни, ліпідтрансферні про-теїни (LTP), циклотиди, снейкіни, гевеїни та кнотини [5,10].Однією із груп АМП рослин, для якої властивий широкий спектр біологічної активності, є ліпідтрансферні протеїни. Молекула LTP рослин характеризується невеликим розміром (7-9 кДа), позитивним зарядом і специфічним мотивом із восьми залишків цистеїну. Третинну структуру LTP формують чотири α-спіральні ланцюги, які стабілізовані дисульфідними зв'язками, та довгий С-кінцевий сегмент. Спіральні ділянки з'єднані між собою рухливими петлями і утворюють гідрофобну кишеню, яка забезпечує взаємодію LTP з жирними кислотами та фосфоліпідами. Ліпідтрансферні протеїни рослин, на відміну від гомологічних їм протеїнів тварин, володіють низькою специфічністю до ліпідів, за що отримали назву "неспецифічні ліпідтрансферні протеїни -нсLTP" [2,13,17].Встановлено, що LTP регулюють внутрішньоклітинний міжмембранний тран-спорт ліпідів, а також залучені до процесів ембріогенезу, росту і розвитку рослин, Biol. Stud. 2012: 6(2);

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Rice lipid transfer protein (LTP) genes belong to a complex multigene family and are differentially regulated

Cited by 66 publications

References 23 publications

Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths

Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths

Increased Accumulation of Cuticular Wax and Expression of Lipid Transfer Protein in Response to Periodic Drying Events in Leaves of Tree Tobacco

Patterns of expression of lipid-transfer protein gene in organs of Scots pine (Pinus sylvestris L.)

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