Rice OsWRKY50 Mediates ABA-Dependent Seed Germination and Seedling Growth, and ABA-Independent Salt Stress Tolerance

Huang, Shuangzhan; Hu, Lanjuan; Zhang, Shihan; Zhou, Mingxing; Jiang, Wenzhu; Wu, Tao; Du, Xinglin

doi:10.3390/ijms22168625

Cited by 39 publications

(31 citation statements)

References 34 publications

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“…Gene structure analysis revealed natural mutations in HcWRKY genes, for instance, some WRKYGQK domains were altered to WRKYGKK, WRKYGEK and WRKYGQE ( Supplementary Figure 2 ). This result coincided with the structural characteristics of WRKY genes of Arabidopsis , rice and wheat ( Rushton et al, 2010 ; Jiang et al, 2012 ; Niu et al, 2012 ; Huang et al, 2021 ). The variations in WRKY gene architecture align with its functional diversity ( Bakshi and Oelmuller, 2014 ; Zhu, 2016 ; Wani et al, 2021 ), and/or the acquisition of a novel function during the evolutionary progress.…”

Section: Discussionsupporting

confidence: 77%

Genomic insights of the WRKY genes in kenaf (Hibiscus cannabinus L.) reveal that HcWRKY44 improves the plant’s tolerance to the salinity stress

et al. 2022

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The WRKY transcription factors (TFs) are among the most diverse TF families of plants. They are implicated in various processes related to plant growth and stress response. Kenaf (Hibiscus cannabinus L.), an important fiber crop, has many applications, including the phytoremediation of saline-alkaline soil. However, the roles of WRKY TFs in kenaf are rarely studied. In the present study, 46 kenaf WRKY genes were genome-widely identified and characterized by gene structure, phylogeny and expression pattern analysis. Furthermore, the HcWRKY44 gene was functionally characterized in Arabidopsis under salinity and drought stresses. HcWRKY44 is a nuclear-localized protein that is positively induced by salinity and drought, with roots showing maximum accumulation of its transcripts. Under NaCl and abscisic acid (ABA) stress conditions, plants overexpressing HcWRKY44 had higher germination rates, better root growth and increased survival than control plants; however, it did not improve the ability to withstand drought stress. Moreover, ABA signaling genes (ABI1, ABI2, and ABI5), ABA-responsive genes (ABF4, RD29B, COR15A, COR47, and RD22), stress-related genes (STZ, P5CS, and KIN1), and ionic homeostasis-related genes (SOS1, AHA1, AHA2, and HKT1) were positively induced in HcWRKY44 transgenic plants under NaCl treatment. These results suggest that HcWRKY44 improved plant’s tolerance to salt stress but not osmotic stress through an ABA-mediated pathway. In summary, this study provides provided comprehensive information about HcWRKY genes and revealed that HcWRKY44 is involved in salinity tolerance and ABA signaling.

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Section: Discussionsupporting

confidence: 77%

Genomic insights of the WRKY genes in kenaf (Hibiscus cannabinus L.) reveal that HcWRKY44 improves the plant’s tolerance to the salinity stress

et al. 2022

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“…The ectopic expressions of TaWRKY75 -A and ZmWRKY79 in Arabidopsis improve the survival rate under drought stress by regulating ABA biosynthesis ( Gulzar et al, 2021 ; Ye et al, 2021 ). OsWRKY50 enhances salt stress tolerance via an ABA-independent pathway ( Huang et al, 2021 ). In order to explore the CsWRKY Group III gene family, we elucidated the function of CsWRKY18 in drought stress through regulating ABA biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Wei

Jin²,

Gao³

et al. 2022

Front. Plant Sci.

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Transcription factors (TFs) of the WRKY family play pivotal roles in defense responses and secondary metabolism of plants. Although WRKY TFs are well documented in numerous plant species, no study has performed a genome-wide investigation of the WRKY gene family in Cymbidium sinense. In the present work, we found 64 C. sinense WRKY (CsWRKY) TFs, and they were further divided into eight subgroups. Chromosomal distribution of CsWRKYs revealed that the majority of these genes were localized on 16 chromosomes, especially on Chromosome 2. Syntenic analysis implied that 13 (20.31%) genes were derived from segmental duplication events, and 17 orthologous gene pairs were identified between Arabidopsis thaliana WRKY (AtWRKY) and CsWRKY genes. Moreover, 55 of the 64 CsWRKYs were detectable in different plant tissues in response to exposure to plant hormones. Among them, Group III members were strongly induced in response to various hormone treatments, indicating their potential essential roles in hormone signaling. We subsequently analyzed the function of CsWRKY18 in Group III. The CsWRKY18 was localized in the nucleus. The constitutive expression of CsWRKY18 in Arabidopsis led to enhanced sensitivity to ABA-mediated seed germination and root growth and elevated plant tolerance to abiotic stress within the ABA-dependent pathway. Overall, our study represented the first genome-wide characterization and functional analysis of WRKY TFs in C. sinense, which could provide useful clues about the evolution and functional description of CsWRKY genes.

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“…For ABA treatment, seeds of both WT and transgenic DH were surface-sterilized by using 4% sodium hypochlorite for 15 min followed by 4–5 washes with autoclaved water. The seeds were allowed to dry inside the laminar air flow and were then plated onto media containing 5 µM ABA and 7 µM ABA [ 38 , 39 ]. The phenotype was observed after 4 and 7 days, respectively.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of HVA1 Enhances Drought and Heat Stress Tolerance in Triticum aestivum Doubled Haploid Plants

Samtani

Sharma

Khurana

2022

Cells

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Plant responses to multiple environmental stresses include various signaling pathways that allow plant acclimation and survival. Amongst different stresses, drought and heat stress severely affect growth and productivity of wheat. HVA1, a member of the group 3 LEA protein, has been well known to provide protection against drought stress. However, its mechanism of action and its role in other stresses such as heat remain unexplored. In this study, doubled haploid (DH) wheat plants overexpressing the HVA1 gene were analyzed and found to be both drought-and heat stress-tolerant. The transcriptome analysis revealed the upregulation of transcription factors such as DREB and HsfA6 under drought and heat stress, respectively, which contribute toward the tolerance mechanism. Particularly under heat stress conditions, the transgenic plants had a lower oxidative load and showed enhanced yield. The overexpression lines were found to be ABA-sensitive, therefore suggesting the role of HsfA6 in providing heat tolerance via the ABA-mediated pathway. Thus, apart from its known involvement in drought stress, this study highlights the potential role of HVA1 in the heat stress signaling pathway. This can further facilitate the engineering of multiple stress tolerance in crop plants, such as wheat.

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Rice OsWRKY50 Mediates ABA-Dependent Seed Germination and Seedling Growth, and ABA-Independent Salt Stress Tolerance

Cited by 39 publications

References 34 publications

Genomic insights of the WRKY genes in kenaf (Hibiscus cannabinus L.) reveal that HcWRKY44 improves the plant’s tolerance to the salinity stress

Genomic insights of the WRKY genes in kenaf (Hibiscus cannabinus L.) reveal that HcWRKY44 improves the plant’s tolerance to the salinity stress

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Overexpression of HVA1 Enhances Drought and Heat Stress Tolerance in Triticum aestivum Doubled Haploid Plants

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