Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells

Horie, Tomoaki; Sugawara, Mitsuo; Okada, Tomoyuki; Taira, Koichiro; Kaothien-Nakayama, Pulla; Katsuhara, Maki; Shinmyo, Atsuhiko; Nakayama, Hideki

doi:10.1016/j.jbiosc.2010.10.014

Cited by 116 publications

(88 citation statements)

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“…In leaf blades, Based on the fluorescence signal of GFP fused to the C terminus of OsHAK5, we detected OsHAK5 expression in the plasma membrane in BY2 cells and in rice protoplasts (Supplemental Fig. S1), consistent with its localization in onion (Allium cepa) epithelial cells (Horie et al, 2011).…”

Section: The Effect Of K Supply On Oshak5 Expression In Ricesupporting

confidence: 58%

“…It has been reported that the transcripts of OsHAK5 were faint and not significantly affected by prolonged (8-11 d) K starvation in rice roots (Okada et al, 2008), while its transcripts in shoots were enhanced by more than 5-fold (Horie et al, 2011). To resolve the effect of short-term K deprivation on OsHAK5 expression, we detected the change of its transcripts by quantitative reverse transcription (qRT)-PCR between 1 and 72 h after removal of K from culture solution.…”

Section: The Effect Of K Supply On Oshak5 Expression In Ricementioning

confidence: 98%

“…OsHAK5 was reported to complement the growth defects of both the K transport E. coli mutant and the high-affinity K uptake-deficient yeast mutant (Horie et al, 2011). We examined the functional complementation of OsHAK5 in a K uptake-deficient yeast strain, R5421, in a series of K concentrations and found that OsHAK5 improved the yeast growth at the [K] range of 0.05 to 10 mM (Supplemental Fig.…”

Section: The Effect Of Oshak5 Expression Level On Direct K Acquisitiomentioning

confidence: 99%

“…Transporters of cluster I, such as AtHAK5, HvHAK1, OsHAK1, and OsHAK5, are localized in the plasma membrane (Kim et al, 1998;Bañuelos et al, 2002;Gierth et al, 2005) and exhibit high-affinity K uptake in the yeast Saccharomyces cerevisiae (Santa-María et al, 1997;Fu and Luan, 1998;Rubio et al, 2000) and in Escherichia coli (Horie et al, 2011). Transporters of cluster II, like AtKUP4 (TINY ROOT HAIRS1, TRH1), HvHAK2, OsHAK2, OsHAK7, and OsHAK10, could not complement the K uptakedeficient yeast (Saccharomyces cerevisiae) but were able to mediate K fluxes in a bacterial mutant; they might be tonoplast transporters (Senn et al, 2001;Bañuelos et al, 2002;Rodríguez-Navarro and Rubio, 2006).…”

mentioning

confidence: 99%

“…The cluster I transporters of HvHAK1 mediate Na influx (Santa-María et al, 1997), while AtHAK5 expression is inhibited by Na NievesCordones et al, 2010). Expression of OsHAK5 in tobacco (Nicotiana tabacum) BY2 cells enhanced the salt tolerance of these cells by accumulating more K without affecting their Na content (Horie et al, 2011).…”

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confidence: 99%

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The Role of a Potassium Transporter OsHAK5 in Potassium Acquisition and Transport from Roots to Shoots in Rice at Low Potassium Supply Levels

Yang

Song²,

Hu³

et al. 2014

Plant Physiology

290

325

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In plants, K transporter (KT)/high affinity K transporter (HAK)/K uptake permease (KUP) is the largest potassium (K) transporter family; however, few of the members have had their physiological functions characterized in planta. Here, we studied OsHAK5 of the KT/HAK/KUP family in rice (Oryza sativa). We determined its cellular and tissue localization and analyzed its functions in rice using both OsHAK5 knockout mutants and overexpression lines in three genetic backgrounds. A β-glucuronidase reporter driven by the OsHAK5 native promoter indicated OsHAK5 expression in various tissue organs from root to seed, abundantly in root epidermis and stele, the vascular tissues, and mesophyll cells. Net K influx rate in roots and K transport from roots to aerial parts were severely impaired by OsHAK5 knockout but increased by OsHAK5 overexpression in 0.1 and 0.3 mm K external solution. The contribution of OsHAK5 to K mobilization within the rice plant was confirmed further by the change of K concentration in the xylem sap and K distribution in the transgenic lines when K was removed completely from the external solution. Overexpression of OsHAK5 increased the K-sodium concentration ratio in the shoots and salt stress tolerance (shoot growth), while knockout of OsHAK5 decreased the K-sodium concentration ratio in the shoots, resulting in sensitivity to salt stress. Taken together, these results demonstrate that OsHAK5 plays a major role in K acquisition by roots faced with low external K and in K upward transport from roots to shoots in K-deficient rice plants.

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Section: The Effect Of K Supply On Oshak5 Expression In Ricesupporting

confidence: 58%

Section: The Effect Of K Supply On Oshak5 Expression In Ricementioning

confidence: 98%

Section: The Effect Of Oshak5 Expression Level On Direct K Acquisitiomentioning

confidence: 99%