Rice Transcription Factor OsWRKY55 Is Involved in the Drought Response and Regulation of Plant Growth

Huang, Kai; Wu, Tao; Ma, Ziming; Zhao, Li; Chen, Haoyuan; Zhang, Mingxing; Bian, Mingdi; Bai, Huijiao; Jiang, Wenzhu; Du, Xinglin

doi:10.3390/ijms22094337

Cited by 32 publications

(15 citation statements)

References 49 publications

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“…In Chickpea ( Cicer arietinum L.), 15 genes were down-regulated in roots and 10 genes were down-regulated in stems under abiotic stress [ 59 ]. The OsWRKY55 negatively regulates drought tolerance in transgenic rice lines [ 60 ], The transcription level of HvWRKY42 increased in Barley ( Hordeum vulgare L.) under drought stress, which indicated that HvWRKY42 was regulating the drought adaptability of barley [ 61 ]. The HvWRKY3 showed 13 fold high expression and 4 genes showed 2-fold high expression under drought stress [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress

Zhu

et al. 2022

BMC Plant Biol

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Background Liriodendron chinense (Lchi) is a tree species within the Magnoliaceae family and is considered a basal angiosperm. The too low or high temperature or soil drought will restrict its growth as the adverse environmental conditions, thus improving L. chinense abiotic tolerance was the key issues to study. WRKYs are a major family of plant transcription factors known to often be involved in biotic and abiotic stress responses. So far, it is still largely unknown if and how the LchiWRKY gene family is tied to regulating L. chinense stress responses. Therefore, studying the involvement of the WRKY gene family in abiotic stress regulation in L. chinense could be very informative in showing how this tree deals with such stressful conditions. Results In this research, we performed a genome-wide analysis of the Liriodendron chinense (Lchi) WRKY gene family, studying their classification relationships, gene structure, chromosomal locations, gene duplication, cis-element, and response to abiotic stress. The 44 members of the LchiWRKY gene family contain a significant amount of sequence diversity, with their lengths ranging from 525 bp to 40,981 bp. Using classification analysis, we divided the 44 LchiWRKY genes into three phylogenetic groups (I, II, II), with group II then being further divided into five subgroups (IIa, IIb, IIc, IId, IIe). Comparative phylogenetic analysis including the WRKY families from 17 plant species suggested that LchiWRKYs are closely related to the Magnolia Cinnamomum kanehirae WRKY family, and has fewer family members than higher plants. We found the LchiWRKYs to be evenly distributed across 15 chromosomes, with their duplication events suggesting that tandem duplication may have played a major role in LchiWRKY gene expansion model. A Ka/Ks analysis indicated that they mainly underwent purifying selection and distributed in the group IId. Motif analysis showed that LchiWRKYs contained 20 motifs, and different phylogenetic groups contained conserved motif. Gene ontology (GO) analysis showed that LchiWRKYs were mainly enriched in two categories, i.e., biological process and molecular function. Two group IIc members (LchiWRKY10 and LchiWRKY37) contain unique WRKY element sequence variants (WRKYGKK and WRKYGKS). Gene structure analysis showed that most LchiWRKYs possess 3 exons and two different types of introns: the R- and V-type which are both contained within the WRKY domain (WD). Additional promoter cis-element analysis indicated that 12 cis-elements that play different functions in environmental adaptability occur across all LchiWRKY groups. Heat, cold, and drought stress mainly induced the expression of group II and I LchiWRKYs, some of which had undergone gene duplication during evolution, and more than half of which had three exons. LchiWRKY33 mainly responded to cold stress and LchiWRKY25 mainly responded to heat stress, and LchiWRKY18 mainly responded to drought stress, which was almost 4-fold highly expressed, while 5 LchiWRKYs (LchiWRKY5, LchiWRKY23, LchiWRKY14, LchiWRKY27, and LchiWRKY36) responded equally three stresses with more than 6-fold expression. Subcellular localization analysis showed that all LchiWRKYs were localized in the nucleus, and subcellular localization experiments of LchiWRKY18 and 36 also showed that these two transcription factors were expressed in the nucleus. Conclusions This study shows that in Liriodendron chinense, several WRKY genes like LchiWRKY33, LchiWRKY25, and LchiWRKY18, respond to cold or heat or drought stress, suggesting that they may indeed play a role in regulating the tree’s response to such conditions. This information will prove a pivotal role in directing further studies on the function of the LchiWRKY gene family in abiotic stress response and provides a theoretical basis for popularizing afforestation in different regions of China.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress

Zhu

et al. 2022

BMC Plant Biol

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“…Six OsWRKYs (OsWRKY5, OsWRKY11, OsWRKY30, OsWRKY45, OsWRKY47, and OsWRKY55) are known regulators of drought tolerance in rice [ 15 , 16 , 17 , 18 , 19 , 20 ]. To examine the relationships among these TFs, we constructed a phylogenetic tree.…”

Section: Resultsmentioning

confidence: 99%

“…Rice ( Oryza sativa L.), which is one of the most important monocot crops, has 103 WRKY TFs, including 28 Group III members [ 13 , 14 ]. Among rice WRKY TFs, OsWRKY5, OsWRKY45, and OsWRKY55 decrease drought tolerance, while OsWRKY11, OsWRKY30, and OsWRKY47 increase it [ 15 , 16 , 17 , 18 , 19 , 20 ]. OsWRKY45, OsWRKY47, and OsWRKY55 are WRKY Group III TFs.…”

Section: Introductionmentioning

confidence: 99%

OsWRKY114 Negatively Regulates Drought Tolerance by Restricting Stomatal Closure in Rice

Song

Son

Lee

et al. 2022

Plants

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The WRKY family of transcription factors plays a pivotal role in plant responses to biotic and abiotic stress. The WRKY Group III transcription factor OsWRKY114 is a positive regulator of innate immunity against Xanthomonas oryzae pv. oryzae; however, its role in abiotic stress responses is largely unknown. In this study, we showed that the abundant OsWRKY114 transcripts present in transgenic rice plants are reduced under drought conditions. The overexpression of OsWRKY114 significantly increased drought sensitivity in rice, which resulted in a lower survival rate after drought stress. Moreover, we showed that stomatal closure, which is a strategy to save water under drought, is restricted in OsWRKY114-overexpressing plants compared with wild-type plants. The expression levels of PYR/PYL/RCAR genes, such as OsPYL2 and OsPYL10 that confer drought tolerance through stomatal closure, were also markedly lower in the OsWRKY114-overexpressing plants. Taken together, these results suggest that OsWRKY114 negatively regulates plant tolerance of drought stress via inhibition of stomatal closure, which would otherwise prevent water loss in rice.

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“…Moreover, MAPK can interact with other transcription factors in response to adversity. OsWRKY55 interacts with four MAPKs that could be induced by drought: OsMPK7 , OsMPK9 , OsMPK20-1 , and OsMPK20-4 [ 14 ]. MdMYB1 can be phosphorylated by MdMPK4 to enhance light-induced anthocyanin accumulation in apples [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of MAPK gene family members in Fagopyrum tataricum and their expression during development and stress responses

et al. 2022

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Background Mitogen-activated protein kinases (MAPKs) plays essential roles in the development, hormone regulation and abiotic stress response of plants. Nevertheless, a comprehensive study on MAPK family members has thus far not been performed in Tartary buckwheat. Results Here, we identified 16 FtMAPKs in the Fagopyrum tataricum genome. Phylogenetic analysis showed that the FtMAPK family members could be classified into Groups A, B, C and D, in which A, B and C members contain a Thr-Glu-Tyr (TEY) signature motif and Group D members contain a Thr-Asp-Tyr (TDY) signature motif. Promoter cis-acting elements showed that most ProFtMAPks contain light response elements, hormone response elements and abiotic stress response elements, and several ProFtMAPks have MYB-binding sites, which may be involved in the regulation of flavonoid biosynthesis-related enzyme gene expression. Synteny analysis indicated that FtMAPKs have a variety of biological functions. Protein interaction prediction suggested that MAPKs can interact with proteins involved in development and stress resistance. Correlation analysis further confirmed that most of the FtMAPK genes and transcription factors involved in the stress response have the same expression pattern. The transient transformation of FtMAPK1 significantly increased the antioxidant enzymes activity in Tartary buckwheat leaves. In addition, we also found that FtMAPK1 can respond to salt stress by up-regulating the transcription abundance of downstream genes. Conclusions A total of 16 MAPKs were identified in Tartary buckwheat, and the members of the MAPK family containing the TDY motif were found to have expanded. The same subfamily members have relatively conserved gene structures and similar protein motifs. Tissue-specific expression indicated that the expression of all FtMAPK genes varied widely in the roots, stems, leaves and flowers. Most FtMAPKs can regulate the expression of other transcription factors and participate in the abiotic stress response. Our findings comprehensively revealed the FtMAPK gene family and laid a theoretical foundation for the functional characterization of FtMAPKs.

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Rice Transcription Factor OsWRKY55 Is Involved in the Drought Response and Regulation of Plant Growth

Cited by 32 publications

References 49 publications

Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress

Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress

OsWRKY114 Negatively Regulates Drought Tolerance by Restricting Stomatal Closure in Rice

Genome-wide identification of MAPK gene family members in Fagopyrum tataricum and their expression during development and stress responses

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