Rice transcriptome analysis to identify possible herbicide quinclorac detoxification genes

Abstract

Quinclorac is a highly selective auxin-type herbicide and is widely used in the effective control of barnyard grass in paddy rice fields, improving the world's rice yield. The herbicide mode of action of quinclorac has been proposed, and hormone interactions affecting quinclorac signaling has been identified. Because of widespread use, quinclorac may be transported outside rice fields with the drainage waters, leading to soil and water pollution and other environmental health problems. In this study, we used 57K Affymetrix rice whole-genome array to identify quinclorac signaling response genes to study the molecular mechanisms of action and detoxification of quinclorac in rice plants. Overall, 637 probe sets were identified with differential expression levels under either 6 or 24 h of quinclorac treatment. Auxin-related genes such as GH3 and OsIAAs responded to quinclorac treatment. Gene Ontology analysis showed that genes of detoxification-related family genes were significantly enriched, including cytochrome P450, GST, UGT, and ABC and drug transporter genes. Moreover, real-time RT-PCR analysis showed that top candidate genes of P450 families such as CYP81, CYP709C, and CYP72A were universally induced by different herbicides. Some Arabidopsis genes of the same P450 family were up-regulated under quinclorac treatment. We conducted rice whole-genome GeneChip analysis and the first global identification of quinclorac response genes. This work may provide potential markers for detoxification of quinclorac and biomonitors of environmental chemical pollution.

Fenoxaprop‐P‐ethyl resistance conferred by cytochrome P450s and target site mutation in Alopecurus japonicus

“…reported that a P450 gene, CYP72A31 , conferred tolerance to ALS‐inhibiting herbicides in rice and Arabidopsis. CYP72A was also found to be associated with pyroxsulam resistance in Lolium species, and quinclorac tolerance in rice . The CYP72A subfamily is conserved in angiosperms, contributing to secondary metabolism .…”

Expression Profile of Defense Genes in Rice Lines Pyramided with Resistance Genes Against Bacterial Blight, Fungal Blast and Insect Gall Midge

“…Among the four genes implicated in plant-insect interactions, Cytochrome P450 family, transposon protein (LOC_Os10g37160) is a member of the large family of genes. Plant genes of this family are also reported to be involved in herbicide tolerance (Xu et al 2015 ), chemical defense and hormone biosynthesis. In our earlier study another copy of Cytochrome P450 gene (Os03g0658800) was found upregulated five fold in rice variety Suraksha with Gm11 gene at both 24 and 120 hai with GM biotype1 (Rawat et al 2013 ).…”

“…LOC_Os04g27430 and LOC_Os08g07100 are reported to be induced by BPH feeding and influence antixenosis in rice Rathu Heenati (Kamolsukyunyong et al 2013 ). The specific gene under study, LOC_Os08g07080 was found induced following fall armyworm feeding on leaves of Nipponbare japonica rice (Yuan et al 2008 ) and also reported to be involved in detoxification of auxin-type herbicide quinchlorac (Xu et al 2015 ).…”

Recurrent Selection by Herbicide Sublethal Dose and Drought Stress Results in Rapid Reduction of Herbicide Sensitivity in Junglerice

“…Increases in UGT expression after florpyrauxifen-benzyl, glyphosate, and quinclorac treatments indicate the involvement of this gene in the adaptation response to these herbicides. In rice, the glucosyltransferase gene was reported to be involved in the glucose conjugation of phenolics and possible quinclorac detoxification [107,108]. A glucosyltransferase was identified and enabled as a potential transcriptional marker for quick diagnosis of metabolic resistance to mesosulfuron in Alopecurus aequalis [18].…”