Rickettsia-like-organisms and phytoplasmas associated with diseases in Australian strawberries

Streten, Claire; Waite, G. K.; Herrington, M. E.; Hutton, D. G.; Persley, D. M.; Gibb, Karen S.

doi:10.1071/ap05007

Cited by 13 publications

(13 citation statements)

References 20 publications

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“…RLO-associated disease was only identified in two non-crop plant hosts in the area surrounding runner production farms where this organism is the most common agent associated with SLY disease (Streten et al 2005b). This suggests that the vector has a limited host range or only a limited number of plants are susceptible to the RLO (Lee et al 1998;Lee et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Ca. P. australiense PYL variant strain has previously been identified as being associated with SLY diseased at Caboolture and it was thought that this strain represented an isolated population within Australia (Streten et al 2005b). The detection of Ca.…”

Section: Discussionmentioning

confidence: 99%

“…P. australiense AGY and PYL variant strains were detected in different G. physocarpus plants collected at Nambour and these plants were growing within 100 m of SLY diseased plants that tested positive for Ca. P. australiense AGY strain (Streten et al 2005b). Ca.…”

Section: Discussionmentioning

confidence: 99%

“…Deoxyribonucleic acid samples were also tested using the PCR primers that amplify the flavoprotein subunit of succinate dehydrogenase (sdhA) gene (PBTF1 and PBTR1) of the RLO associated with lethal yellows (Streten et al 2005b) and PBT disease (Davis et al 1998). PCR reactions were prepared according to Davis et al (1998).…”

Section: Screening For Phytoplasmas and Rickettsia-like-organismsmentioning

confidence: 99%

“…Little is known about this RLO, as until recently there was no diagnostic test. The development of a PCR diagnostic test, which amplifies the flavoprotein subunit succinate dehydrogenase (sdhA) gene of the SLY RLO (Streten et al 2005b) and the papaya bunchy top (PBT) RLO, has facilitated the identification of other hosts and possible vectors for this organism. The only known vector of an RLO is the leafhopper, Empoasca papayae, which transmits the PBT RLO (Davis et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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Plant hosts of the phytoplasmas and rickettsia-like-organisms associated with strawberry lethal yellows and green petal diseases

Streten

Herrington²,

Hutton³

et al. 2005

Austral. Plant Pathol.

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Abstract. Candidatus Phytoplasma australiense (Ca. P. australiense) is associated with the plant diseases strawberry lethal yellows (SLY), strawberry green petal (SGP), papaya dieback (PDB), Australian grapevine yellows (AGY) and Phormium yellow leaf (PYL; New Zealand). Strawberry lethal yellows disease is also associated with a rickettsia-like-organism (RLO) or infrequently with the tomato big bud (TBB) phytoplasma, the latter being associated with a wide range of plant diseases throughout Australia. In contrast, the RLO has been identified only in association with SLY disease, and Ca. P. australiense has been detected only in a limited number of plant host species. The aim of this study was to identify plant hosts that are possible reservoirs of Ca. P. australiense and the SLY RLO. Thirty-one plant species from south-east Queensland were observed with disease between 2001 and 2003 and, of these, 18 species tested positive using phytoplasma-specific primers. The RLO was detected in diseased Jacksonia scoparia and Modiola caroliniana samples collected at Stanthorpe. The TBB phytoplasma was detected in 16 different plant species and Ca. P. australiense Australian grapevine yellows strain was detected in six species. The TBB phytoplasma was detected in plants collected at Nambour, Stanthorpe, Warwick and Brisbane. Ca. P. australiense was detected in plants collected at Nambour, Stanthorpe, Gatton and Allora. All four phytoplasmas were detected in diseased Gomphocarpus physocarpus plants collected at Toowoomba, Allora, Nambour and Gatton. These results indicated that the vector(s) of Ca. P. australiense are distributed throughout south-east Queensland and the diversity of phytoplasmas detected in G. physocarpus suggests it is a feeding source for phytoplasma insect vectors or it has a broad susceptibility to a range of phytoplasmas.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Screening For Phytoplasmas and Rickettsia-like-organismsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plant hosts of the phytoplasmas and rickettsia-like-organisms associated with strawberry lethal yellows and green petal diseases

Streten

Herrington²,

Hutton³

et al. 2005

Austral. Plant Pathol.

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Molecular Techniques for Detection of Microbial Pathogens

Narayanasamy

Molecular Biology in Plant Pathogenesis and Disease Management

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Detection, identification and differentiation of microbial plant pathogens -oomycetes, fungi, bacteria, phytoplasmas, viruses and viroids -infecting various crops constitute the basic step for the development of effective crop disease management systems. The conventional cultural methods involving isolation and studying the morphological characteristics using microscope are labor intensive and cumbersome, yielding sometimes, inconclusive results. The molecular techniques, on the other hand, are able to provide precise, reliable and reproducible results rapidly, facilitating early disease management decisions. Biochemical, immunological and nucleic acid-based assays have been preferred, because of the distinct advantages over the conventional methods. The molecular techniques have been very useful in the identification of obligate pathogens causing diseases such as downy mildews, powdery mildews and rusts and also fungi that grow very slowly in the culture media, taking several weeks to produce spore forms that can be used for identification. The availability of antisera, primers and commercial kits has led to widespread application of the molecular techniques for on-site detection during field surveys for assessing the distribution of existing pathogens, occurrence of new/introduced pathogens or strains and for preventing introduction of new pathogens through seeds or planting materials. Furthermore, molecular techniques have been demonstrated to be useful in breeding programs to identify sources of resistance to disease(s). Several protocols for molecular techniques, useful for researchers and students are presented in the Appendix.

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