Rif1 Regulates the Fate of DNA Entanglements during Mitosis

Zaaijer, Sophie; Shaikh, Nadeem; Nageshan, Rishi Kumar; Cooper, Julia Promisel

doi:10.1016/j.celrep.2016.05.077

Cited by 34 publications

(66 citation statements)

References 45 publications

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“…Thus, localization of Rif1 should be important for regulation of replication timing. Interestingly, Rif1‐EGFP exhibits a cloud‐like tailed localization around a defined focus of Taz1‐mCherry in G1/S cells (Appendix Fig S5), as reported previously (Zaaijer et al , ). It is possible that PP1 is enriched around the telomeres forming a phosphatase dominant “PP1‐zone” (Fig ).…”

Section: Discussionsupporting

confidence: 86%

Shelterin promotes tethering of late replication origins to telomeres for replication‐timing control

et al. 2018

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DNA replication initiates at many discrete loci on eukaryotic chromosomes, and individual replication origins are regulated under a spatiotemporal program. However, the underlying mechanisms of this regulation remain largely unknown. In the fission yeast , the telomere-binding protein Taz1, ortholog of human TRF1/TRF2, regulates a subset of late replication origins by binding to the telomere-like sequence near the origins. Here, we showed using a/LacI-GFP system that Taz1-dependent late origins were predominantly localized at the nuclear periphery throughout interphase, and were localized adjacent to the telomeres in the G1/S phase. The peripheral localization that depended on the nuclear membrane protein Bqt4 was not necessary for telomeric association and replication-timing control of the replication origins. Interestingly, the shelterin components Rap1 and Poz1 were required for replication-timing control and telomeric association of Taz1-dependent late origins, and this requirement was bypassed by a minishelterin Tpz1-Taz1 fusion protein. Our results suggest that Taz1 suppresses replication initiation through shelterin-mediated telomeric association of the origins at the onset of S phase.

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Section: Discussionsupporting

confidence: 86%

Shelterin promotes tethering of late replication origins to telomeres for replication‐timing control

et al. 2018

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“…It is also consistent with the finding that RIF1 associates with Lamin B1 (Foti et al, 2016). A role for RIF1 in safeguarding the integrity replication factories may also explain how RIF1 controls the activation of dormant origins in response to replicative stress (Hiraga, Ly et al, 2017), and prevents the formation of anaphase bridges (Hengeveld, de Boer et al, 2015, Zaaijer, Shaikh et al, 2016. RIF1 depletion has a strong impact on replication timing (Cornacchia et al, 2012, Foti et al, 2016, Yamazaki et al, 2012, possibly by regulating DDK kinase activation through an interaction with the PP1 phosphatase (Dave et al, 2014, Hiraga et al, 2014, Mattarocci et al, 2014.…”

Section: Discussionsupporting

confidence: 85%

The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress

Ribeyre

Lebdy

Patouillard

et al. 2019

Preprint

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Eukaryotic genomes are duplicated from thousands of replication origins that fire sequentially forming a defined spatiotemporal pattern of replication clusters. The importance of the organization of replisomes into functional clusters, also called replication factories, is still poorly understood. Here we identified the multifunctional protein RIF1 as a structural component of replication factories. RIF1 depletion did not impair the velocity of replication forks, neither in basic conditions nor in presence of a molecule that interferes with replication fork progression but increased the frequency of DNA lesions induced in S phase. Isolation of replication-associated proteins from RIF1-depleted cells revealed a major defect in the clustering of replication factors on nascent DNA, without any noticeable impact on DNA synthesis or replisome stability. We found that the changes in replication patterns commonly observed upon RIF1 depletion are induced by DNA replication stress. The data suggest that RIF1 encases replication factories to ensure the organization of replication clusters against chromatin rearrangements induced by DNA replication stress.

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“…taz1Δ mutant cells accumulate DNA bridges coated with RPA that cannot be resolved at lower temperatures (Zaaijer et al, 2016). We therefore exploited time lapse microscopy in strains expressing Rad11-GFP to establish if rrp2Δ prevented the formation of taz1Δdependent anaphase bridges, or if such bridges were formed in taz1Δrrp2Δ cells, but more efficiently processed.…”

Section: Rrp1 and Rrp2 Influence Centromere Structure By Modulation Omentioning

confidence: 99%

S. pombeDNA translocases Rrp1 and Rrp2 have distinct roles at centromeres and telomeres that ensure genome stability

Barg-Wojas

Schirmeisen

Muraszko

et al. 2019

Preprint

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STATEMENTSchizosaccharomyces pombe DNA translocases Rrp1 and Rrp2 modulate centromere and telomere maintenance pathways and dysregulation of their activity leads to genome instability. ABSTRACTHomologous recombination (HR) is a DNA repair mechanism that ensures, together with heterochromatin machinery, the proper replication, structure and function of telomeres and centromeres that is essential for the maintenance of genome integrity. Schizosaccharomyces pombe Rrp1 and Rrp2 participate in HR and are orthologues of Saccharomyces cerevisiae Uls1, a SWI2/SNF2 DNA translocase and SUMO-Targeted Ubiquitin Ligase. We show that Rrp1 or Rrp2 upregulation leads to chromosome instability and growth defects. These phenotypes depend on putative DNA translocase activities of Rrp1 and Rrp2. Either Rrp1 or Rrp2 overproduction results in a reduction in global histone levels, suggesting that Rrp1 and Rrp2 may modulate nucleosome dynamics. In addition we show that Rrp2, but not Rrp1, acts at telomeres. We propose that this role depends on the previously described interaction between Rrp2 and Top2. We conclude that Rrp1 and Rrp2 have important roles for centromere and telomere function and maintenance, contributing to the preservation of genome stability during vegetative cell growth. PLoS Genet. 8, e1002985. Choi, E. S., Cheon, Y., Kang, K. and Lee, D. (2017). The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3. Nat. Commun. 8, 529. . (2009). The role of novel genes rrp1(+) and rrp2(+) in the repair of DNA damage in Schizosaccharomyces pombe. DNA Repair (Amst). 8, 627-636. Involvement of Schizosaccharomyces pombe rrp1+ and rrp2 + in the Srs2-and Swi5/Sfr1-dependent pathway in response to DNA damage and replication inhibition.Allshire, R. C. (1996). Mutations in the fission yeast silencing factors clr4+ and rik1+ disrupt the localisation of the chromo domain protein Swi6p and impair centromere function.

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Rif1 Regulates the Fate of DNA Entanglements during Mitosis

Cited by 34 publications

References 45 publications

Shelterin promotes tethering of late replication origins to telomeres for replication‐timing control

Shelterin promotes tethering of late replication origins to telomeres for replication‐timing control

The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress

S. pombeDNA translocases Rrp1 and Rrp2 have distinct roles at centromeres and telomeres that ensure genome stability

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