Rifampicin treatment of canine pyoderma due to multidrug‐resistant meticillin‐resistant staphylococci: a retrospective study of 32 cases

Lucia, Michela De; Bardagí, Mar; Fabbri, Elisabetta; Ferreira, Diana; Ferrer, L.; Scarampella, Fabia; Zanna, Giordana; Fondati, Alessandra

doi:10.1111/vde.12404

Cited by 14 publications

(16 citation statements)

References 18 publications

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“…Lactating cows from the large and medium organic herds had ARG conferring resistance to rifampins in fecal samples which were not detected in other animal groups but were detected at much lower concentration in conventional herds. Rifampin is used to treat certain types of infections in horses [37] and is occasionally used in dogs and cats [38] but is not prescribed for use in livestock. It has been reported that ARG conferring resistance to rifampin, particularly rpoB, contribute to 35% of the abundance of annotated ARG across diverse environmental samples such as aquaculture sediment, sludge, biofilm, and river water environments [39].…”

Section: Discussionmentioning

confidence: 99%

The distribution of microbiomes and resistomes across farm environments in conventional and organic dairy herds in Pennsylvania

Pitta

Indugu

Toth

et al. 2020

Environmental Microbiome

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Background Antimicrobial resistance is a serious concern. Although the widespread use of antimicrobials in livestock has exacerbated the emergence and dissemination of antimicrobial resistance genes (ARG) in farm environments, little is known about whether antimicrobial use affects distribution of ARG in livestock systems. This study compared the distribution of microbiomes and resistomes (collections of ARG) across different farm sectors in dairy herds that differed in their use of antimicrobials. Feces from heifers, non-lactating, and lactating cows, manure storage, and soil from three conventional (antimicrobials used to treat cows) and three organic (no antimicrobials used for at least four years) farms in Pennsylvania were sampled. Samples were extracted for genomic DNA, processed, sequenced on the Illumina NextSeq platform, and analyzed for microbial community and resistome profiles using established procedures. Results Microbial communities and resistome profiles clustered by sample type across all farms. Overall, abundance and diversity of ARG in feces was significantly higher in conventional herds compared to organic herds. The ARG conferring resistance to betalactams, macrolide-lincosamide-streptogramin (MLS), and tetracyclines were significantly higher in fecal samples of dairy cows from conventional herds compared to organic herds. Regardless of farm type, all manure storage samples had greater diversity (albeit low abundance) of ARG conferring resistance to aminoglycosides, tetracyclines, MLS, multidrug resistance, and phenicol. All soil samples had lower abundance of ARG compared to feces, manure, and lagoon samples and were comprised of ARG conferring resistance to aminoglycosides, glycopeptides, and multi-drug resistance. The distribution of ARG is likely driven by the composition of microbiota in the respective sample types. Conclusions Antimicrobial use on farms significantly influenced specific groups of ARG in feces but not in manure storage or soil samples.

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Section: Discussionmentioning

confidence: 99%

The distribution of microbiomes and resistomes across farm environments in conventional and organic dairy herds in Pennsylvania

Pitta

Indugu

Toth

et al. 2020

Environmental Microbiome

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“…Based on the literature, availability and cost, treatment elected in this case was combination therapy with clarithromycin and rifampin. Dosing of rifampin was based on recent articles providing dose ranges of 2.9–16 mg/kg/day and 10 mg/kg/day, with significant elevations of ALT potentially occurring at three to four weeks following start of therapy 21 22. In this case no significant elevations of ALT occurred; however, increases in ALP were noted and are thought to be secondary to liver enzyme induction 21…”

Section: Discussionmentioning

confidence: 99%

Cutaneous manifestation of Mycobacterium avium complex infection in an Australian shepherd dog

Ramos

Woodward

Wakamatsu

et al. 2019

Vet Record Case Reports

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A three-year-old male neutered Australian shepherd dog presented with a one-week history of diffuse ulcerative cutaneous nodules. On physical examination the dog had generalised lymphadenomegaly with multiple well-circumscribed subcutaneous to dermal nodules with central haemorrhagic ulceration affecting the face, dorsum, dorsal cervical area and abdomen. Histopathological findings revealed severe suppurative and pyogranulomatous dermatitis and panniculitis with acid-fast pleomorphic bacterium present. Mycobacterial culture of tissue was negative. Repeated PCR assays on formalin-fixed tissue followed by nucleic acid sequencing confirmed the presence of Mycobacterium avium complex. Treatment was initiated with clarithromycin (10.9 mg/kg orally every 12 hours) and rifampin (13.2 mg/kg orally every 24 hours) and resulted in clinical resolution of the lesions within two weeks. Treatment was continued for six months and then discontinued. Seventeen months following treatment discontinuation, the dog was clinically and systemically well with no relapse noted.

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“…The synergy with vancomycin was also demonstrated on human patients against MRSA septicemia in burns (61), in a knee prosthetic infection model (62) and in the treatment of nosocomial MRSA induced pneumonia (63). In addition to these examples, several other studies using different animal models and/or other types of infection have also shown good synergistic effects of rifampicin with other antibiotics such as linezolid (64), b-lactams or glycopeptides (65), and some topical antimicrobials (66).Yet, other results provided no overall benefit of the adjunctive effect of rifampicin over standard antibiotic therapy against S. aureus; neither in vitro (67,68). In addition, the use of rifampicin adjunctive therapy for the treatment of SSTI is not recommended by the Infectious Diseases Society of America (69).…”

Section: Discussionmentioning

confidence: 91%

A Strong Synergy Between the Thiopeptide Bacteriocin Micrococcin P1 and Rifampicin Against MRSA in a Murine Skin Infection Model

et al. 2021

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Antibiotic-resistant bacterial pathogens have become a serious threat worldwide. One of these pathogens is methicillin-resistant Staphylococcus aureus (MRSA), a major cause of skin and soft tissue infections. In this study we identified a strain of Staphylococcus equorum producing a substance with high antimicrobial activity against many Gram-positive bacteria, including MRSA. By mass spectrometry and whole genome sequencing the antimicrobial substance was identified as the thiopeptide bacteriocin micrococcin P1 (MP1). Based on its properties we developed a one-step purification protocol resulting in high yield (15 mg/L) and high purity (98%) of MP1. For shorter incubation times (5-7 h) MP1 was very potent against MRSA but the inhibitory effect was overshadowed by resistance development during longer incubation time (24h or more). To overcome this problem a synergy study was performed with a number of commercially available antibiotics. Among the antibiotics tested, the combination of MP1 and rifampicin gave the best synergistic effect, with MIC values 25 and 60 times lower than for the individual drugs, respectively. To assess the therapeutic potential of the MP1-rifampicin combination, we used a murine skin infection model based on the use of the multidrug-resistant luciferase-tagged MRSA strain Xen31. As expected, neither of the single antimicrobials (MP1 or rifampicin) could eradicate Xen31 from the wounds. By contrary, the MP1-rifampicin combination was efficient not only to eradicate but also to prevent the recurrence of Xen31 infection. Furthermore, compared to fucidin cream, which is commonly used in skin infection treatments, MP1-rifampicin combination was superior in terms of preventing resistance development. Our results show that combining MP1, and probably other thiopeptides, with antibiotics can be a promising strategy to treat SSTIs caused by MRSA and likely many other Gram-positive bacteria.

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Rifampicin treatment of canine pyoderma due to multidrug‐resistant meticillin‐resistant staphylococci: a retrospective study of 32 cases

Cited by 14 publications

References 18 publications

The distribution of microbiomes and resistomes across farm environments in conventional and organic dairy herds in Pennsylvania

The distribution of microbiomes and resistomes across farm environments in conventional and organic dairy herds in Pennsylvania

Cutaneous manifestation of Mycobacterium avium complex infection in an Australian shepherd dog

A Strong Synergy Between the Thiopeptide Bacteriocin Micrococcin P1 and Rifampicin Against MRSA in a Murine Skin Infection Model

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