Right ventricular metabolic adaptations to high-intensity interval and moderate-intensity continuous training in healthy middle-aged men

Heiskanen, Marja A.; Leskinen, Tuija; Heinonen, Ilkka; Löyttyniemi, Eliisa; Eskelinen, Jari-Joonas; Virtanen, Kirsi A.; Hannukainen, Jarna C.; Kalliokoski, Kari K.

doi:10.1152/ajpheart.00399.2016

Cited by 20 publications

(22 citation statements)

References 45 publications

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“…Regardless of the development of new tracers, [ 18 F]FTHA is still widely used. At Turku PET Centre, we have used [ 18 F]FTHA in numerous studies of fatty acid metabolism in, for example, the myocardium, adipose tissue function, and obesity …”

Section: Introductionmentioning

confidence: 99%

Automated production of [¹⁸F]FTHA according to GMP

Savisto

Viljanen

Kokkomäki

et al. 2018

Labelled Comp Radiopharmac

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14-(R,S)-[ F]fluoro-6-thia-heptadecanoic acid is a tracer for fatty acid imaging by positron emission tomography. High demand for this tracer required us to replace semiautomatic synthesis with a fully automated procedure. An automated synthesis device was constructed in-house for multistep nucleophilic F-fluorination and a control system was developed. The synthesis device was combined with a sterile filtration unit and both were qualified. 14-(R,S)-[ F]fluoro-6-thia-heptadecanoic acid was produced according to good manufacturing practice guidelines set by the European Union. The synthesis includes an initial nucleophilic labelling reaction, deprotection, preparative HPLC separation, purification of the final product, and formulation for injection. The duration and temperature of the reaction and hydrolysis were optimized, and the radiochemical stability of the formulated product was determined. The rotary evaporator used to evaporate the solvent after HPLC purification was replaced with solid phase extraction purification. We also replaced the human serum albumin used in the earlier procedure with a phosphate buffer-ascorbic acid mixture in the final formulation solution. From 2011 to 2016, we performed 219 synthesis procedures, 94% of which were successful. The radiochemical yield of 14-(R,S)-[ F]fluoro-6-thia-heptadecanoic acid, decay-corrected to the end of bombardment, was 13% ± 6.3%. The total amount of formulated end product was 1.7 ± 0.8 GBq at end of synthesis.

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Section: Introductionmentioning

confidence: 99%

Automated production of [¹⁸F]FTHA according to GMP

Savisto

Viljanen

Kokkomäki

et al. 2018

Labelled Comp Radiopharmac

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“…We have previously published several reports from this study (9,10,16,17,24,25,37). The main parameters of the present study, epi- and pericardial fat masses and MTC, have not been published before neither in healthy nor in DGT subjects.…”

Section: Methodsmentioning

confidence: 91%

Exercise Training Reduces Intrathoracic Fat Regardless of Defective Glucose Tolerance

Honkala

Motiani

Eskelinen

et al. 2017

Medicine &Amp; Science in Sports &Amp; Exercise

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PurposeEpicardial (EAT) and pericardial (PAT) fat masses and myocardial triglyceride content (MTC) are enlarged in obesity and insulin resistance. We studied whether the high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) similarly decrease ectopic fat in and around the heart and whether the decrease is similar in healthy subjects and subjects with defective glucose tolerance (DGT).MethodsA total of 28 healthy men (body mass index = 20.7–30.0 kg·m−2, age = 40–55 yr) and 16 men with DGT (body mass index = 23.8–33.5 kg·m−2, age = 43–53 yr) were randomized into HIIT and MICT interventions for 2 wk. EAT and PAT were determined by computed tomography and MTC by 1H-MRS.ResultsAt baseline, DGT subjects had impaired aerobic capacity and insulin sensitivity and higher levels of whole body fat, visceral fat, PAT, and EAT (P < 0.05, all) compared with healthy subjects. In the whole group, HIIT increased aerobic capacity (HIIT = 6%, MICT = 0.3%; time × training P = 0.007) and tended to improve insulin sensitivity (HIIT = 24%, MICT = 8%) as well as reduce MTC (HIIT = −42%, MICT = +23%) (time × training P = 0.06, both) more efficiently compared with MICT, and without differences in the training response between the healthy and the DGT subjects. However, both training modes decreased EAT (−5%) and PAT (−6%) fat (time P < 0.05) and not differently between the healthy and the DGT subjects.ConclusionWhole body fat, visceral fat, PAT, and EAT masses are enlarged in DGT. Both HIIT and MICT effectively reduce EAT and PAT in healthy and DGT subjects, whereas HIIT seems to be superior as regards improving aerobic capacity, whole-body insulin sensitivity, and MTC.

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“…The training interventions consisted of six exercise sessions within two weeks 28 , 31 – 34 . Each session was performed in supervised laboratory conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Full details of the protocols used to determine the outcome measures of this study have been described elsewhere 29 , 31 , 32 and are briefly summarized here. The number of completed and uncompleted experiments for the outcome measures are detailed in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

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Sprint interval training decreases left-ventricular glucose uptake compared to moderate-intensity continuous training in subjects with type 2 diabetes or prediabetes

Heiskanen

Sjöros

Heinonen

et al. 2017

Sci Rep

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Type 2 diabetes mellitus (T2DM) is associated with reduced myocardial glucose uptake (GU) and increased free fatty acid uptake (FFAU). Sprint interval training (SIT) improves physical exercise capacity and metabolic biomarkers, but effects of SIT on cardiac function and energy substrate metabolism in diabetic subjects are unknown. We tested the hypothesis that SIT is more effective than moderate-intensity continuous training (MICT) on adaptations in left and right ventricle (LV and RV) glucose and fatty acid metabolism in diabetic subjects. Twenty-six untrained men and women with T2DM or prediabetes were randomized into two-week-long SIT (n = 13) and MICT (n = 13) interventions. Insulin-stimulated myocardial GU and fasted state FFAU were measured by positron emission tomography and changes in LV and RV structure and function by cardiac magnetic resonance. In contrast to our hypothesis, SIT significantly decreased GU compared to MICT in LV. FFAU of both ventricles remained unchanged by training. RV end-diastolic volume (EDV) and RV mass increased only after MICT, whereas LV EDV, LV mass, and RV and LV end-systolic volumes increased similarly after both training modes. As SIT decreases myocardial insulin-stimulated GU compared to MICT which may already be reduced in T2DM, SIT may be metabolically less beneficial than MICT for a diabetic heart.

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Right ventricular metabolic adaptations to high-intensity interval and moderate-intensity continuous training in healthy middle-aged men

Cited by 20 publications

References 45 publications

Automated production of [¹⁸F]FTHA according to GMP

Automated production of [¹⁸F]FTHA according to GMP

Exercise Training Reduces Intrathoracic Fat Regardless of Defective Glucose Tolerance

Sprint interval training decreases left-ventricular glucose uptake compared to moderate-intensity continuous training in subjects with type 2 diabetes or prediabetes

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Right ventricular metabolic adaptations to high-intensity interval and moderate-intensity continuous training in healthy middle-aged men

Cited by 20 publications

References 45 publications

Automated production of [18F]FTHA according to GMP

Automated production of [18F]FTHA according to GMP

Exercise Training Reduces Intrathoracic Fat Regardless of Defective Glucose Tolerance

Sprint interval training decreases left-ventricular glucose uptake compared to moderate-intensity continuous training in subjects with type 2 diabetes or prediabetes

Contact Info

Product

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Automated production of [¹⁸F]FTHA according to GMP

Automated production of [¹⁸F]FTHA according to GMP