Ring-opening polymerization of ε-caprolactone initiated by the antitumor agent doxifluridine

Chang, Kuo-Yung; Lee, Yu‐Der

doi:10.1016/j.actbio.2008.11.010

Cited by 23 publications

(9 citation statements)

References 42 publications

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“…To functionalize polymer assemblies using the previously described azide + alkyne click chemistry approach, new PEO‐PCL block copolymers bearing terminal azides on the hydrophilic PEO blocks were required. As the polymerization of ε‐caprolactone is generally initiated from small molecule 48, 49 or macromolecular alcohols, 50–52 these target copolymers could be most readily derived from asymmetrically functionalized PEOs bearing azide and hydroxyl termini (N 3 ‐PEO‐OH). While there are numerous reports describing the asymmetric functionalization of oligo(ethylene glycol)s, 53–55 their higher MW analogues, particularly those lacking charged moieties are more difficult to prepare due to the purification challenges associated with statistical functionalization reactions.…”

Section: Resultsmentioning

confidence: 99%

Regulation of the interferon‐α production induced by RNA‐containing immune complexes in plasmacytoid dendritic cells

Eloranta¹,

Lövgren²,

Finke³

et al. 2009

Arthritis & Rheumatism

118

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Objective. Interferon-␣ (IFN␣) is produced in several autoimmune diseases, including systemic lupus erythematosus (SLE), and may be important in their pathogenesis. We undertook this study to investigate how IFN␣ production induced by RNA-containing immune complexes (ICs) in plasmacytoid dendritic cells (PDCs) is regulated.Methods. Normal PDCs purified from peripheral blood mononuclear cells (PBMCs) were cocultivated with other cell populations isolated from healthy individuals or SLE patients. IFN␣ production was induced by RNA-containing ICs, which consisted of anti-RNP autoantibodies and U1 small nuclear RNP particles, and the effects of prostaglandin E 2 (PGE 2 ), reactive oxygen species (ROS), or the cytokines IFN␣2b, granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-10 (IL-10), or tumor necrosis factor ␣ (TNF␣) were explored.Results. Monocytes inhibited IFN␣ production by PDCs in PBMC cultures, while natural killer (NK) cells were stimulatory. The monocytes had little effect on IFN␣ production by pure PDCs but inhibited its stimulation by NK cells. Monocytes from SLE patients were less inhibitory. Exposure of PBMCs or PDCs to IFN␣2b/GM-CSF increased their IFN␣ production. RNA-containing ICs caused production of ROS, PGE 2 , and TNF␣, especially in monocytes. These mediators and IL-10 suppressed IFN␣ production in PBMC cultures, with ROS and PGE 2 also inhibiting IFN␣ production by purified PDCs. Inhibition by all of these agents, except for ROS, was abolished by IFN␣2b/GM-CSF. The inhibitory effect of monocytes was significantly counteracted by the ROS scavengers serotonin and catalase.Conclusion. IFN␣ production induced by RNAcontaining ICs in PDCs is regulated by a network of interactions between monocytes, NK cells, and PDCs, involving several pro-and antiinflammatory molecules. This should be considered when designing and applying new therapies.

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Section: Resultsmentioning

confidence: 99%

Regulation of the interferon‐α production induced by RNA‐containing immune complexes in plasmacytoid dendritic cells

Eloranta¹,

Lövgren²,

Finke³

et al. 2009

Arthritis & Rheumatism

118

View full text Add to dashboard Cite

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“…Amphiphilic PEG-PCL copolymer was synthesized by ring opening polymerization of ε-CL monomer using macroinitiator of PEG and catalyst of tin (II) 2-ethylhexanoate as reported previously [28, 29]. The prepared polymer was characterized by a Bruker AM 400 proton nuclear magnetic resonance ( 1 H-NMR) (Bruker Co., Billerica, USA) at 400 MHz using deuterated chloroform (CDCl 3 ) as solvent.…”

Section: Methodsmentioning

confidence: 99%

Biodistribution of biodegradable polymeric nano-carriers loaded with busulphan and designed for multimodal imaging

Asem

Ying

et al. 2016

J Nanobiotechnol

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BackgroundMultifunctional nanocarriers for controlled drug delivery, imaging of disease development and follow-up of treatment efficacy are promising novel tools for disease diagnosis and treatment. In the current investigation, we present a multifunctional theranostic nanocarrier system for anticancer drug delivery and molecular imaging. Superparamagnetic iron oxide nanoparticles (SPIONs) as an MRI contrast agent and busulphan as a model for lipophilic antineoplastic drugs were encapsulated into poly (ethylene glycol)-co-poly (caprolactone) (PEG-PCL) micelles via the emulsion-evaporation method, and PEG-PCL was labelled with VivoTag 680XL fluorochrome for in vivo fluorescence imaging.ResultsBusulphan entrapment efficiency was 83% while the drug release showed a sustained pattern over 10 h. SPION loaded-PEG-PCL micelles showed contrast enhancement in T 2*-weighted MRI with high r 2* relaxivity. In vitro cellular uptake of PEG-PCL micelles labeled with fluorescein in J774A cells was found to be time-dependent. The maximum uptake was observed after 24 h of incubation. The biodistribution of PEG-PCL micelles functionalized with VivoTag 680XL was investigated in Balb/c mice over 48 h using in vivo fluorescence imaging. The results of real-time live imaging were then confirmed by ex vivo organ imaging and histological examination. Generally, PEG-PCL micelles were highly distributed into the lungs during the first 4 h post intravenous administration, then redistributed and accumulated in liver and spleen until 48 h post administration. No pathological impairment was found in the major organs studied.ConclusionsThus, with loaded contrast agent and conjugated fluorochrome, PEG-PCL micelles as biodegradable and biocompatible nanocarriers are efficient multimodal imaging agents, offering high drug loading capacity, and sustained drug release. These might offer high treatment efficacy and real-time tracking of the drug delivery system in vivo, which is crucial for designing of an efficient drug delivery system.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-016-0239-0) contains supplementary material, which is available to authorized users.

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“…Based on the generally accepted conclusion, the number-average molecular weights determined by 1 H NMR spectroscopy (M n(NMR) ) were calculated from the average chain length (DP) and average degree of substitution (DS) of the resulting homopolymers [22]. The calculation results as well as SEC-MALLS data are shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of genistein-containing star-shaped homo- and copolyesters by the ring-opening polymerization

Olędzka

2013

Polym. Bull.

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A novel initiator, naturally occurring in soy, has been developed for a direct synthesis of aliphatic star-shaped homo-and copolyesters. The synthesis is based on one of several known isoflavones, genistein, which has been employed as an initiator of homopolymerization of poly(L-Lactide) or co-initiator of copolymerization of e-caprolactone and L-Lactide with Sn(Oct) 2 as a catalyst. The nontoxic chemicals used allow for an inexpensive and safe for human body facile synthesis of biocompatible functionalized polymers suitable for medical and pharmaceutical applications. The obtained polymers were characterized by 1 H, 13 C NMR, FT-IR, SEC-MALLS and MALDI-TOF MS analysis.

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Ring-opening polymerization of ε-caprolactone initiated by the antitumor agent doxifluridine

Cited by 23 publications

References 42 publications

Regulation of the interferon‐α production induced by RNA‐containing immune complexes in plasmacytoid dendritic cells

Regulation of the interferon‐α production induced by RNA‐containing immune complexes in plasmacytoid dendritic cells

Biodistribution of biodegradable polymeric nano-carriers loaded with busulphan and designed for multimodal imaging

Synthesis of genistein-containing star-shaped homo- and copolyesters by the ring-opening polymerization

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