Ring-Shaped Assembly of Microtubules Shows Preferential Counterclockwise Motion

Microtubule (MT) and kinesin are rail and motor proteins that are involved in various moving events of eukaryotic cells in natural systems. In vitro, the sliding motion of microtubules (rail) can be reproduced on a kinesin (motor protein)-coated surface coupled with adenosine triphosphate (ATP) hydrolysis, which is called a "motility assay". Based on this technique, a method was recently established to form MT assemblies by an active self-organization (AcSA) process, in which MTs are crosslinked during a sliding motion on a kinesin-coated surface. Streptavidin (ST) was employed as glue to crosslink biotin-labeled MTs. Various shapes, sizes, and motilities were formed with the AcSA MT assemblies, depending on the initial conditions. In this paper, we briefly review our recent work on the formation of MT assemblies on a kinesin-coated surface.pg. 2

show abstract

“…2-fold higher flexural rigidity [ref. 40,41]. Using GMPCPP-MTs, the MT bundles were successfully formed.…”

Section: Integration Of the Gliding Mtsmentioning

confidence: 99%

Formation of motile assembly of microtubules driven by kinesins

Kawamura

Kakugo

Shikinaka

et al. 2011

Smart Mater. Struct.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, as shown in Fig. 8, an MT with a left-or right-handed supertwist shows axial rotation when moving over processive motor protein 18 . This axial rotation is assumed to be superimposed on the linear motion, generating a torque on the MT that leads to bending of the front segment of MT.…”

Section: Resultsmentioning

confidence: 54%

“…As shown in this figure, any obvious change in the distribution of the inner diameters of the rings moving CCW and CW cannot be found. Previously, it was shown that on a kinesin-coated surface, the direction of the rotational motion of ring-shaped MT assemblies could be tuned from CCW to CW by using structure-controlled MTs 16,18 .…”

Section: Resultsmentioning

confidence: 99%

Formation of ring-shaped microtubule assemblies through active self-organization on dynein

Ito

Kabir

Inoue

et al. 2013

Polym J

Self Cite

View full text Add to dashboard Cite

Abstract:Microtubule (MT)-kinesin, a biomolecular motor system, is a promising candidate for construction of artificial biomachines for a variety of nanotechnology applications. An active self-organization (AcSO) method involving a specific streptavidin (St)-biotin (Bt) interaction has been developed to assemble MTs into a highly ordered structure by exploiting their motility on a kinesin-coated surface. Dynein is another biomolecular motor that moves along the MTs in the opposite direction from kinesin. Dynein has not yet been used to demonstrate the AcSO of MTs. In this study, we report the first successful demonstration of the AcSO of MTs on a dynein-coated surface to produce ring-shaped MT assemblies similar to those of kinesin. We found that ring-shaped MT assemblies obtained on dynein showed equal clockwise and counterclockwise rotational motion. This work will enrich the building blocks for designing future oriented motor protein-based artificial devices.Introduction:

show abstract

“…interaction to prevent the sliding of the MTs 31 . In this case also MTs were assembled into rings, but unlike the rings produced at the air-buffer interface, MTs in those rings had no chance of sliding past each other due to strong binding between St and Bt.…”

mentioning

confidence: 99%

Mechanical oscillation of dynamic microtubule rings

et al. 2016

Self Cite

View full text Add to dashboard Cite

Mechanical oscillation is a ubiquitous phenomenon observed in living systems, which emerges from a wide range of well-organized self-assembled structures, and plays important roles in many biological processes. Although considerable efforts have been devoted to demonstrate the mechanical oscillation of organized structures produced through self-assembly in vitro, it has rarely been documented. Here we report the mechanical oscillation of ring-shaped structures, composed of multiple microtubule (MT) filaments, obtained through energy dissipative self-assembly of MT filaments at an air-buffer interface.The MT rings exhibit autonomous oscillation manifested through periodic changes in the size and shape. We propose the oscillation of the MT rings is attributed to a mechanical feedback arising from accumulated stress induced by the driving force of the motor protein system. This work might offer new insights in our current understanding on the mechanical feedback driven oscillation of organized structures and its effect on the dynamic processes in living systems.3

show abstract

Ring-Shaped Assembly of Microtubules Shows Preferential Counterclockwise Motion

Cited by 75 publications

References 22 publications

Formation of motile assembly of microtubules driven by kinesins

Formation of motile assembly of microtubules driven by kinesins

Formation of ring-shaped microtubule assemblies through active self-organization on dynein

Mechanical oscillation of dynamic microtubule rings

Contact Info

Product

Resources

About