Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior

Abstract

Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functions. Whether local or rinsing administration of vitamin C alters gingival fibroblast wound healing behavior remains unclear. The aim of this study was to investigate the rinsing effect of vitamin C on gingival fibroblast behavior utilizing an in vitro wound healing model. Primary human gingival fibroblasts isolated from gingival tissue were rinsed with medium containing various concentrations of vitamin C. The rinsing effect of vitamin C on in vitro wound healing was assessed using a scratch test assay. Cell migration, cell viability, and extracellular matrix gene expression were analyzed by transwell migration assay, MTT assay, and real-time RT-PCR, respectively. We found that rinsing with 10 or 20 µg/ml vitamin C significantly increased fibroblast migration (p≤0.05). However, no significant effect was found in the cell viability or in vitro wound healing assays. In contrast, rinsing with 50 µg/ml vitamin C significantly delayed wound closure (p≤0.05). Real-time PCR demonstrated that 50 µg/ml vitamin C significantly increased fibroblast expression of COL1, FN, IL-6, and bFGF. The data demonstrate that rinsing with vitamin C (10/20 µg/ml) accelerates fibroblast migration. However, 50 µg/ml of vitamin C increases the expression of COL1, FN, IL-6, and bFGF, which are related to fibroblast wound healing activity. Prescribing vitamin C with the appropriate duration and drug administration method should be determined to maximize its benefit.

Vitamin C stimulates RNA expression of human gingival fibroblasts proliferation and adhesion in cigarette smokers: An in vitro study

“…Even with induced vitamin C, the gene was not significantly upregulated compared to control hGFs, regardless of the smoking condition. In contrast to the results obtained by Chaitrakoonthong et al, rinsing hGFs with vit C led to a significant increase in COL1 gene expression ( Chaitrakoonthong et al, 2020 ). Because their methodology does not coincide with ours, the comparison is inaccurate.…”

Taking 200 mg Vitamin C Three Times per Day Improved Extraction Socket Wound Healing Parameters: A Randomized Clinical Trial

“…The definitive vitamin C regimen is currently unresolved, and there are no specific guidelines for prescribing vitamin C for wound healing [ 18 ]. Our previous in vitro study revealed that a lower dose of vitamin C promoted fibroblast migration and in vitro wound closure [ 12 ]. Moreover, the absorption of oral vitamin C was 100% at a dose of 200 mg, the absorption at doses of 500 mg and 1,250 mg were <75% and <50%, respectively [ 13 ].…”

“…Two studies reported that 500 mg vitamin C taken orally 3 or 4 times/day (1,500 mg/d and 2,000 mg/d) promoted extraction wound healing [ 10 , 11 ]. In contrast, our in vitro study found that rinsing with a low dose (20 μ g/ml) of vitamin C induced better fibroblast migration, proliferation, and gingival wound closure compared with a higher dose (50 μ g/ml) of vitamin C. Moreover, the higher dose of vitamin C reduced cell viability in oral fibroblast culture [ 12 ]. The absorption of oral vitamin C was highest at a dose of 200 mg [ 13 ].…”

Ultrasound-Assisted Synthesis, Antioxidant Activity and Computational Study of 1,3,4-Oxadiazol-2-amines

“…2 Vitamin C is required for the proper functioning of some enzymes and immune system, tissue regeneration and the enzymatic production. [3][4][5] Oil-soluble vitamin E includes four tocopherols as well as four tocotrienols. It is found in cereals, vegetable oils, meat, poultry, fruits and eggs, and its deficiency damages the nervous system.…”