RIP140 Gene and Protein Expression Levels are Downregulated in Visceral Adipose Tissue in Human Morbid Obesity

Catalán, Victoria; Gómez-Ambrosi, Javier; Lizanzu, Amaia; Rodríguez, Amaia; Silva, Camilo; Rotellar, Fernando; Gil, Marı́a J.; Cienfuegos, Javier A.; Salvador, Javier; Frühbeck, Gema

doi:10.1007/s11695-009-9834-6

Cited by 10 publications

(20 citation statements)

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“…Moreover, the corepressor Rip140, that plays a key role in energy homeostasis by repressing metabolic gene networks [49], was dramatically reduced in iNOS knockout and ob/ob mice. These data are in line with previous studies in humans [50], supporting the notion that downregulation of Rip140 may be a compensatory mechanism in order to favour energy expenditure and fat accumulation reduction in already established obese states.…”

Section: Discussionsupporting

confidence: 92%

Deletion of Inducible Nitric-Oxide Synthase in Leptin-Deficient Mice Improves Brown Adipose Tissue Function

et al. 2010

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BackgroundLeptin and nitric oxide (NO) on their own participate in the control of non-shivering thermogenesis. However, the functional interplay between both factors in this process has not been explored so far. Therefore, the aim of the present study was to analyze the impact of the absence of the inducible NO synthase (iNOS) gene in the regulation of energy balance in ob/ob mice.Methods and FindingsDouble knockout (DBKO) mice simultaneously lacking the ob and iNOS genes were generated, and the expression of molecules involved in the control of brown fat cell function was analyzed by real-time PCR, western-blot and immunohistochemistry. Twelve week-old DBKO mice exhibited reduced body weight (p<0.05), decreased amounts of total fat pads (p<0.05), lower food efficiency rates (p<0.05) and higher rectal temperature (p<0.05) than ob/ob mice. Ablation of iNOS also improved the carbohydrate and lipid metabolism of ob/ob mice. DBKO showed a marked reduction in the size of brown adipocytes compared to ob/ob mutants. In this sense, in comparison to ob/ob mice, DBKO rodents showed an increase in the expression of PR domain containing 16 (Prdm16), a transcriptional regulator of brown adipogenesis. Moreover, iNOS deletion enhanced the expression of mitochondria-related proteins, such as peroxisome proliferator-activated receptor γ coactivator-1 α (Pgc-1α), sirtuin-1 (Sirt-1) and sirtuin-3 (Sirt-3). Accordingly, mitochondrial uncoupling proteins 1 and 3 (Ucp-1 and Ucp-3) were upregulated in brown adipose tissue (BAT) of DBKO mice as compared to ob/ob rodents.ConclusionAblation of iNOS improved the energy balance of ob/ob mice by decreasing food efficiency through an increase in thermogenesis. These effects may be mediated, in part, through the recovery of the BAT phenotype and brown fat cell function improvement.

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Section: Discussionsupporting

confidence: 92%

Deletion of Inducible Nitric-Oxide Synthase in Leptin-Deficient Mice Improves Brown Adipose Tissue Function

et al. 2010

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“…As has previously been reported in visceral WAT [6], abdominal subcutaneous RIP140 mRNA levels were reduced in obesity. Considering the functions ascribed to lack of RIP140 in in vitro studies, i.e.…”

Section: Discussionsupporting

confidence: 78%

“…In mice it appears that the RIP140 is involved in glucose metabolism and energy expenditure of WAT [3-5,16,17]. We have shown that RIP140 mRNA levels in subcutaneous WAT, as has previously been reported for visceral WAT [6], are inversely correlated with measures of adiposity and are enriched in fat cells of adipose tissue. Moreover, RIP140 reduce basal glucose transport and expression of the genes GLUT4 and UCP -1.…”

Section: Discussionsupporting

confidence: 64%

“…According to record GDS596 RIP140 mRNA is widely expressed in different human tissues with particularly high expression levels observed in lung, skeletal muscle, reproductive organs and brain. It has recently been reported that RIP140 mRNA and protein levels are decreased in visceral WAT of morbidly obese as compared to lean humans implying that human RIP140 may, just as its rodent orthologue, regulate adipose tissue metabolism [6]. However, the function and expression of RIP140 mRNA in human subcutaneous WAT, which comprises the main store of body fat, has to our knowledge not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Role of Receptor-Interacting Protein 140 in human fat cells

et al. 2010

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BackgroundMice lacking Receptor-interacting protein 140 (RIP140) have reduced body fat which at least partly is mediated through increased lipid and glucose metabolism in adipose tissue. In humans, RIP140 is lower expressed in visceral white adipose tissue (WAT) of obese versus lean subjects. We investigated the role of RIP140 in human subcutaneous WAT, which is the major fat depot of the body.MethodsMessenger RNA levels of RIP140 were measured in samples of subcutaneous WAT from women with a wide variation in BMI and in different human WAT preparations. RIP140 mRNA was knocked down with siRNA in in vitro differentiated adipocytes and the impact on glucose transport and mRNA levels of target genes determined.ResultsRIP140 mRNA levels in subcutaneous WAT were decreased among obese compared to lean women and increased by weight-loss, but did not associate with mitochondrial DNA copy number. RIP140 expression increased during adipocyte differentiation in vitro and was higher in isolated adipocytes compared to corresponding pieces of WAT. Knock down of RIP140 increased basal glucose transport and mRNA levels of glucose transporter 4 and uncoupling protein-1.ConclusionsHuman RIP140 inhibits glucose uptake and the expression of genes promoting energy expenditure in the same fashion as the murine orthologue. Increased levels of human RIP140 in subcutaneous WAT of lean subjects may contribute to economize on energy stores. By contrast, the function and expression pattern does not support that RIP140 regulate human obesity.

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“…Moreover, NRIP1 is involved in some human disease processes, like morbid obesity (Catalán et al, 2009) and breast cancer (Docquier et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Tetra-primer ARMS-PCR identified a missense mutation of the bovine <i>NRIP1</i> gene associated with growth traits

Liu

Wang

et al. 2015

Arch. Anim. Breed.

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Abstract. Nuclear receptor-interacting protein 1 (NRIP1) specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors to inhibit transcription. Previous work has demonstrated this protein to be a key regulator in modulating transcriptional activity of many transcription factors, some of which are closely related to development and growth. In this study, we have successfully genotyped two newly identified bovine NRIP1 single-nucleotide polymorphisms (SNPs) (c.605A>G and c.1301G>A) using the T-ARMS-PCR method and validated the accuracy by means of PCR-RFLP assay using 1809 individuals of 9 different cattle breeds. The association analyses results indicated that c.605A>G locus was significantly associated with body weight and average daily gain in Nanyang cattle at 18 months (P < 0.05). Thus it can be inferred that T-ARMS-PCR is a rapid, reliable, and cheap method for SNP genotyping and that c.605A>G polymorphism in bovine NRIP1 is associated with growth traits. These findings will be of benefit for the application of DNA markers related to growth traits in marker-assisted selection (MAS), and will improve the promotion of beef cattle.

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RIP140 Gene and Protein Expression Levels are Downregulated in Visceral Adipose Tissue in Human Morbid Obesity

Abstract: The lower gene and protein expression levels of RIP140 in obese subjects may suggest a compensatory mechanism in order to favor energy expenditure and reduce fat accumulation in obesity states.

Cited by 10 publications

References 43 publications

Deletion of Inducible Nitric-Oxide Synthase in Leptin-Deficient Mice Improves Brown Adipose Tissue Function

Deletion of Inducible Nitric-Oxide Synthase in Leptin-Deficient Mice Improves Brown Adipose Tissue Function

Role of Receptor-Interacting Protein 140 in human fat cells

Tetra-primer ARMS-PCR identified a missense mutation of the bovine <i>NRIP1</i> gene associated with growth traits

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RIP140 Gene and Protein Expression Levels are Downregulated in Visceral Adipose Tissue in Human Morbid Obesity

Abstract: The lower gene and protein expression levels of RIP140 in obese subjects may suggest a compensatory mechanism in order to favor energy expenditure and reduce fat accumulation in obesity states.

Cited by 10 publications

References 43 publications

Deletion of Inducible Nitric-Oxide Synthase in Leptin-Deficient Mice Improves Brown Adipose Tissue Function

Deletion of Inducible Nitric-Oxide Synthase in Leptin-Deficient Mice Improves Brown Adipose Tissue Function

Role of Receptor-Interacting Protein 140 in human fat cells

Tetra-primer ARMS-PCR identified a missense mutation of the bovine &lt;i&gt;NRIP1&lt;/i&gt; gene associated with growth traits

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Tetra-primer ARMS-PCR identified a missense mutation of the bovine <i>NRIP1</i> gene associated with growth traits