RIPs Interact with OsFH5 and Control the Rice Morphology by Affecting Stability of OsFH5

Abstract

E3 ubiquitin ligases have been more widely reported to mediate the degradation of target proteins. In our research, one SIAH type E3 ligase was identified, named RIP1, and there are five genes were found to be highly homologous to it, named RIP2 to RIP6 dividedly. Y2H and BiFC were carried out to prove the interaction between RIPs and OsFH5, moreover, homo- and hetero- oligomers can be formed among RIPs and they all can affect the localization of OsFH5. Degradation experiments showed that RIP1 is able to slow down the degradation rate of OsFH5, and also RIP5, RIP6. There was no obvious phenotype in both RIP1 overexpression and mutant transgenic lines, whereas in rips multi-knock out lines showed up similar defects to osfh5, such as, dwarf height; less smooth and rounder seeds; leaves width became wilder. Otherwise, rips also exhibit some special phenotypes, for instance, the angles between leaves and stems were appreciable enlarged; spiral crimped roots. In addition, microfilaments organization in rips appeared disordered than wild type. All above illustrate that RIPs influence morphology by interacting with OsFH5 and affecting its subcellular localization, stability or maybe involved in other pathways in rice.

Genome-Wide Identification and Expression Profiling of the FORMIN Gene Family Implies Their Potential Functions in Abiotic Stress Tolerance in Rice (Oryza sativa)

“…Additionally, OsFH5 was coupled to actin depolymerizing factor 9. And studies had showed that OsFH5 participates in the regulation of rice morphogenesis by modulating the expansion of diffusely growing cells (Chang et al 2021). Thus the possibility that OsFH5 may play a key role in the cell growth and development of rice via depolymerization of the cytoskeleton could not be excluded.…”