Risk factors for deep vein thrombosis of lower extremities in Sudanese women

Ibrahim, Nadir A.; Hassan, Fathelrahman Mahdi; Elgari, Mahmoud Mohamed; Abdalla, Sanaa Eltahir

doi:10.2147/vhrm.s170556

Cited by 9 publications

(9 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding laboratory studies, all our patients presented high hemoglobin levels, a factor linked to increased blood viscosity and, probably, to subsequent thrombotic events [33][34][35]. In addition, our results show that there are different hemostatic alterations in every individual analyzed, presenting out-of-range values in one or more parameters.…”

Section: Discussionmentioning

confidence: 72%

Familial cases of Mexican patients with Legg-Calvé-Perthes disease

Rodríguez-Olivas¹,

Zamora

Rosales-Cruz³

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundLegg-Calvé-Perthes Disease (LCPD) is described as an avascular necrosis of the femoral head. Although its etiology is still not fully understood, evidences suggest heritable thrombotic disorders and other factors may be implicated in its onset and progress. Our objective is to describe, in three enrolled families, the genetic, biochemical and environmental factors that may be associated with the etiology and development of LCPD. MethodsTherefore, we set out to evaluate the following alterations of collagen genes: MTHFR rs1801133, CBS rs115742905, and PT rs1799963 and their relationship with LCPD. Thrombophilia associated markers (FI, FII, FV, FVII, FVIII, FIX, FX, FXI, FXII, FvW, PC, PS, AT, and homocysteine) were evaluated using coagulometry methods. Results: Seven LCPD patients and 14 healthy volunteers were enrolled. Concentrations in hemoglobin (p ≤ 0.0001), fibrinogen (P ≤ 0.0001), homocysteine (p = 0.0414), factor IX activity percentage (p ≤ 0.0001), and protein S (p = 0.0478) showed statistically significant differences. None of the evaluated polymorphisms showed statistically significant differences. However, all patients presented the mutated MTHFR C677T polymorphism in a homozygous (T/T) or heterozygous manner (C/T).ConclusionsOur results show environmental elements from every family and hemostatic disorders may be involved in suffering and developing LCPD. Also, heritable factors could contribute to the onset of the disease. Clearly, environmental, genetic, and prothrombotic factors are involved in this pathology.

show abstract

Section: Discussionmentioning

confidence: 72%

Familial cases of Mexican patients with Legg-Calvé-Perthes disease

Rodríguez-Olivas¹,

Zamora

Rosales-Cruz³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Lower extremity deep vein thrombosis (DVT) is a common peripheral vascular disease, which can lead to venous valve insufficiency and concurrent pulmonary embolism (1). The incidence of thrombosis in the left lower extremity is much higher than that in its right counterpart (2), and primary iliac-femoral vein thrombosis is particularly common. DVT of the lower extremity can also extend to the inferior vena cava, or even block the renal vein to cause renal failure and threaten life (3).…”

Section: Introductionmentioning

confidence: 99%

Expression and role of interleukin‑1β and associated biomarkers in deep vein thrombosis

et al. 2021

View full text Add to dashboard Cite

Lower extremity deep vein thrombosis (DVT) is a common peripheral vascular disease, in which inflammation plays an important role. The aim of the present study was to investigate the expression and role of inflammatory factors in DVT. A rat model of venous thrombosis of the lower extremities was established through venous ligation surgery. The rats were examined at 2, 8, 24, 48 and 72 h after the induction of inferior venous stenosis and compared with control and sham surgery groups. The serum levels of interleukin-1β (IL-1β), tissue factor (TF) and xanthine oxidase (XOD) were measured using ELISAs. The morphology of the DVT tissue was observed by hematoxylin and eosin staining. Circulating endothelial cells (CECs) in peripheral blood were counted by flow cytometry. Reverse transcription-quantitative PCR and western blotting were used to detect mRNA and protein expression, respectively. The serum levels of IL-1β, TF and XOD exhibited no significant differences between the control and sham surgery groups. However, those in the rat model of DVT presented an upward trend from 2 to 24 h and peaked at 24 h, with a significant difference from the respective levels in the control and sham surgery groups. The histopathological analysis revealed the presence of red and mixed thrombi in the rats 2-48 h following the induction of inferior venous stenosis group with inflammatory cell infiltration in the vascular wall. Thrombus formation was evident after 72 h. While significant difference was observed in the number of CECs in the peripheral blood between the control and sham surgery groups, the number of peripheral blood CECs in the rats with inferior venous stenosis group increased from 8 to 72 h, with significant differences among these groups. The mRNA levels of IL-1β, TF, XOD and NF-κB in the tissues peaked at 24 h, with significant differences compared with those in the control and sham surgery groups. In addition, the protein expression level of NF-κB increased from 2 to 72 h. In conclusion, these results suggest that the high expression of IL-1β, TF, XOD and NF-κB may promote thrombus formation.

show abstract

“…1 , 2 The lower extremities are the most common sites for DVT, and the incidence of DVT in lower extremities is about 8-10 times that of the upper extremities. 3 Approximately 500,000 patients with lower extremity venous thrombosis occur in the United States each year, among whom 10% of patients develop extremely fatal pulmonary embolism, 4 20-50% of patients develop postthrombotic syndrome, 5 and 5-10% of patients develop severe post-thrombotic syndrome. 6 In China, there are about 12 million cases every year, much higher than that in the United States.…”

Section: Introductionmentioning

confidence: 99%

“…The prevalence of DVT in the lower extremities of the Kazakhs, Xinjiang, China is about 31.0%, which is much higher than that in other regions of China and other countries in the world. 3 However, the underlying mechanisms for the high prevalence of DVT in the Kazakhs are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Role and Mechanism of mir-5189-3p in Deep Vein Thrombosis of Lower Extremities

Lü

Fang

2021

Annals of Vascular Surgery

View full text Add to dashboard Cite

Background: This study is to investigate the role and mechanism of mir-5189-3p in deep vein thrombosis (DVT) in lower extremity. Methods: The blood samples were collected from Kazakh patients with DVT in lower extremity and were subjected to microRNA sequencing. Bioinformatics were used to identify mir-5189-3p and its target genes. Dual luciferase reporter assay was used to determine the regulatory effect of mir-5189-3p on JAG1. SD rats were randomly divided into normal control, DVT model, hsa-miR-5189-3p mimics and hsa-miR-5189-3p negative control groups. HE staining was used to observe the pathological changes. TUNEL method was used to observe apoptosis. Western blot was used to detect Bax and Bcl-2 protein expression. Real-time quantitative PCR was used to detect JAG1, Notch1 and Hes1 mRNA. Results:The target of Has-miR-5189-3p was JAG1. Co-transfection of miR-5189-3p mimics and pmirGLO/JAG1 wild-type plasmid induced significantly decreased luciferase activity. In hsa-miR-5189-3p mimics and hsa-miR-5189-3p negative control groups, there were more nucleated cells in the thrombus tissues, and the organization degree obviously increased. Signs of blood flow recanalization were observed. The apoptosis of hsa-miR-5189-3p mimics and hsa-miR-5189-3p negative control groups was lower than that in DVT model group. Furthermore, mir-5189-3p mimics significantly increased the mRNA levels of JAG1,Notch1 and Hes1 . Additionally, mir-5189-3p mimics significantly increased Bcl-2 while decreased Bax protein.Conclusions: mir-5189-3p could inhibit apoptosis and promote thrombus organization in DVT possibly via Notch signaling pathway. Mir-5189-3p can be used as a potential target for DVT treatment.

show abstract

Risk factors for deep vein thrombosis of lower extremities in Sudanese women

Cited by 9 publications

References 44 publications

Familial cases of Mexican patients with Legg-Calvé-Perthes disease

Familial cases of Mexican patients with Legg-Calvé-Perthes disease

Expression and role of interleukin‑1β and associated biomarkers in deep vein thrombosis

Role and Mechanism of mir-5189-3p in Deep Vein Thrombosis of Lower Extremities

Contact Info

Product

Resources

About